Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1990 May;70(1):133–135.

The selective augmentation by recombinant human tumour necrosis factor-alpha of neutrophil responses to pathogenic Escherichia coli.

R Steadman 1, M M Petersen 1, D Williams 1, N Matthews 1, J D Williams 1
PMCID: PMC1384094  PMID: 2162324

Abstract

Endotoxin release may amplify the neutrophil (PMN) responses to bacterial infection through the release of monocyte-derived tumour necrosis factor (TNF). The present study was designed to assess the effect of recombinant human TNF-alpha (rhTNF-alpha) on the in vitro response of human PMN to two defined strains of pathogenic Escherichia coli. In the absence of rhTNF-alpha, a P-fimbriate strain caused significant release of the PMN secondary granule marker vitamin B12-binding protein (B12 BP), and a low level of release of leukotriene B4 (LTB4). Type 1-fimbriate strain 504, however, stimulated the release of the primary granule marker myeloperoxidase (MPO) and PMN chemiluminescence (CL), in addition to B12 BP and LTB4 release. Following rhTNF-alpha (10(-9) M) pretreatment, the release of LTB4 by PMN stimulated with the P-fimbriate strain was synergistically augmented, while B12 BP and MPO release were additively increased. In contrast, rhTNF-alpha did not significantly affect any of the responses by the type 1-fimbriate strain. These results suggest selectivity in the priming of PMN by rhTNF-alpha and confirm the independence of PMN responses to phagocytic stimuli.

Full text

PDF
133

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blumenstock E., Jann K. Adhesion of piliated Escherichia coli strains to phagocytes: differences between bacteria with mannose-sensitive pili and those with mannose-resistant pili. Infect Immun. 1982 Jan;35(1):264–269. doi: 10.1128/iai.35.1.264-269.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Harber M. J., Topley N., Asscher A. W. Virulence factors of urinary pathogens. Clin Sci (Lond) 1986 Jun;70(6):531–538. doi: 10.1042/cs0700531. [DOI] [PubMed] [Google Scholar]
  3. Harber M. J., Topley N. Factors affecting the measurement of chemiluminescence in stimulated human polymorphonuclear leucocytes. J Biolumin Chemilumin. 1986 Jun;1(1):15–27. doi: 10.1002/bio.1170010105. [DOI] [PubMed] [Google Scholar]
  4. Movat H. Z., Cybulsky M. I., Colditz I. G., Chan M. K., Dinarello C. A. Acute inflammation in gram-negative infection: endotoxin, interleukin 1, tumor necrosis factor, and neutrophils. Fed Proc. 1987 Jan;46(1):97–104. [PubMed] [Google Scholar]
  5. Rokach J., Hayes E. C., Girard Y., Lombardo D. L., Maycock A. L., Rosenthal A. S., Young R. N., Zamboni R., Zweerink H. J. The development of sensitive and specific radioimmunoassays for leukotrienes. Prostaglandins Leukot Med. 1984 Jan;13(1):21–25. doi: 10.1016/0262-1746(84)90098-2. [DOI] [PubMed] [Google Scholar]
  6. Scheffer J., König W., Hacker J., Goebel W. Bacterial adherence and hemolysin production from Escherichia coli induces histamine and leukotriene release from various cells. Infect Immun. 1985 Oct;50(1):271–278. doi: 10.1128/iai.50.1.271-278.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Slotki I. N., Asscher A. W. Prevention of scarring in experimental pyelonephritis in the rat by early antibiotic therapy. Nephron. 1982;30(3):262–268. doi: 10.1159/000182484. [DOI] [PubMed] [Google Scholar]
  8. Steadman R., Topley N., Jenner D. E., Davies M., Williams J. D. Type 1 fimbriate Escherichia coli stimulates a unique pattern of degranulation by human polymorphonuclear leukocytes. Infect Immun. 1988 Apr;56(4):815–822. doi: 10.1128/iai.56.4.815-822.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Svanborg Edén C., Bjursten L. M., Hull R., Hull S., Magnusson K. E., Moldovano Z., Leffler H. Influence of adhesins on the interaction of Escherichia coli with human phagocytes. Infect Immun. 1984 Jun;44(3):672–680. doi: 10.1128/iai.44.3.672-680.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Topley N., Steadman R., Mackenzie R., Knowlden J. M., Williams J. D. Type 1 fimbriate strains of Escherichia coli initiate renal parenchymal scarring. Kidney Int. 1989 Oct;36(4):609–616. doi: 10.1038/ki.1989.237. [DOI] [PubMed] [Google Scholar]
  11. Williams J. D., Topley N., Alobaidi H. M., Harber M. J. Activation of human polymorphonuclear leucocytes by particulate zymosan is related to both its major carbohydrate components: glucan and mannan. Immunology. 1986 May;58(1):117–124. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES