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. 1990 Jul;70(3):296–302.

Detection and characterization of circulating and glomerular immune complexes in experimental IgA nephropathy.

J González-Cabrero 1, J Egido 1, A Barat 1, E González 1
PMCID: PMC1384156  PMID: 2379938

Abstract

The different models of experimental IgA nephropathy described so far have provided insight into pathogenesis; however, the evidence for a role of IgA immune complexes (IC) has only been gained in passive systems. In an active model of IgA nephropathy, induced in mice by repeated injections of dextran, some of the mechanisms that could explain the formation of glomerular IgA deposits are studied in this report. Serum total IgA and anti-dextran IgA antibody levels increased significantly over the period of immunization. Only 13-30% of mice had total and/or specific IgA IC, determined by Raji cell and PEG assay in ELISA. Analytical ultracentrifugation showed that IgA IC were of small (7-13 S) or intermediate (13-17 S) size. There was a close correlation between total serum IgA levels and the presence of IC-containing IgA anti-dextran antibodies, with the existence of IgA in the mesangium. The percentage of animals (n = 76) with IgA mesangial deposits increased over the immunization period (88% at 10 weeks). Forty-three per cent of mice had polymeric IgA in the mesangium; by contrast, only 12% had dextran deposits. On the whole, these data suggest that in the dextran-induced IgA nephropathy, the glomerular IgA could be the result of circulating IgA complexes and/or IgA polymers deposition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chen A., Wong S. S., Rifai A. Glomerular immune deposits in experimental IgA nephropathy. A continuum of circulating and in situ formed immune complexes. Am J Pathol. 1988 Jan;130(1):216–222. [PMC free article] [PubMed] [Google Scholar]
  2. Czerkinsky C., Koopman W. J., Jackson S., Collins J. E., Crago S. S., Schrohenloher R. E., Julian B. A., Galla J. H., Mestecky J. Circulating immune complexes and immunoglobulin A rheumatoid factor in patients with mesangial immunoglobulin A nephropathies. J Clin Invest. 1986 Jun;77(6):1931–1938. doi: 10.1172/JCI112522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davin J. C., Dechenne C., Mahieu P. R. Glomérulonéphrite aiguë expérimentale induite par le dépôt glomérulaire de complexes circulants IgA polymérique-concanavaline A. Nephrologie. 1989;10(3):151–155. [PubMed] [Google Scholar]
  4. DeFranco A. L., Ashwell J. D., Schwartz R. H., Paul W. E. Polyclonal stimulation of resting B lymphocytes by antigen-specific T lymphocytes. J Exp Med. 1984 Mar 1;159(3):861–880. doi: 10.1084/jem.159.3.861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Egido J., Julian B. A., Wyatt R. J. Genetic factors in primary IgA nephropathy. Nephrol Dial Transplant. 1987;2(3):134–142. [PubMed] [Google Scholar]
  6. Egido J., Sancho J., Mampaso F., Lopez Trascasa M., Sanchez Crespo M., Blasco R., Hernando L. A possible common pathogenesis of the mesangial IgA glomerulonephritis in patients with Berger's disease and Schönlein-Henoch syndrome. Proc Eur Dial Transplant Assoc. 1980;17:660–666. [PubMed] [Google Scholar]
  7. Egido J., Sancho J., Rivera F., Hernando L. The role of IgA and IgG immune complexes in IgA nephropathy. Nephron. 1984;36(1):52–59. doi: 10.1159/000183115. [DOI] [PubMed] [Google Scholar]
  8. Emancipator S. N., Gallo G. R., Razaboni R., Lamm M. E. Experimental cholestasis promotes the deposition of glomerular IgA immune complexes. Am J Pathol. 1983 Oct;113(1):19–26. [PMC free article] [PubMed] [Google Scholar]
  9. Genin C., Laurent B., Sabatier J. C., Colon S., Berthoux F. C. IgA mesangial deposits in C3H/HeJ mice after oral immunization with ferritin or bovine serum albumin. Clin Exp Immunol. 1986 Feb;63(2):385–394. [PMC free article] [PubMed] [Google Scholar]
  10. Gonzalez E., Egido J., Gonzalez-Cabrero J., Sancho J., Blasco R., Garcia-Hoyo R., Lozano L., Barat A. Immunological studies of experimental IgA nephropathy in mice. Adv Exp Med Biol. 1987;216B:1583–1591. [PubMed] [Google Scholar]
  11. González-Cabrero J., Egido J., Sancho J., Moldenhauer F. Presence of shared idiotypes in serum and immune complexes in patients with IgA nephropathy. Clin Exp Immunol. 1987 Jun;68(3):694–702. [PMC free article] [PubMed] [Google Scholar]
  12. González E., González-Cabrero J., Egido J. Defective hepatic handling of IgA immune aggregates by mice with experimental IgA nephropathy. Immunology. 1989 Jul;67(3):308–313. [PMC free article] [PubMed] [Google Scholar]
  13. Iida H., Izumino K., Matsumoto M., Takata M., Mizumura Y., Sugimoto T. Glomerular deposition of IgA in experimental hepatic cirrhosis. Acta Pathol Jpn. 1985 May;35(3):561–567. doi: 10.1111/j.1440-1827.1985.tb00598.x. [DOI] [PubMed] [Google Scholar]
  14. Imai H., Chen A., Wyatt R. J., Rifai A. Composition of IgA immune complexes precipitated with polyethylene glycol. A model for isolation and analysis of immune complexes. J Immunol Methods. 1987 Nov 5;103(2):239–245. doi: 10.1016/0022-1759(87)90295-x. [DOI] [PubMed] [Google Scholar]
  15. Isaacs K. L., Miller F. Antigen size and charge in immune complex glomerulonephritis. II. Passive induction of immune deposits with dextran-anti-dextran immune complexes. Am J Pathol. 1983 Jun;111(3):298–306. [PMC free article] [PubMed] [Google Scholar]
  16. Isaacs K., Miller F., Lane B. Experimental model for IgA nephropathy. Clin Immunol Immunopathol. 1981 Sep;20(3):419–426. doi: 10.1016/0090-1229(81)90152-5. [DOI] [PubMed] [Google Scholar]
  17. Kauffmann R. H., Herrmann W. A., Meÿer C. J., Daha M. R., Van Es L. A. Circulating IgA-immune complexes in Henoch-Schönlein purpura. A longitudinal study of their relationship to disease activity and vascular deposition of IgA. Am J Med. 1980 Dec;69(6):859–866. doi: 10.1016/s0002-9343(80)80011-8. [DOI] [PubMed] [Google Scholar]
  18. Rifai A., Mannik M. Clearance kinetics and fate of mouse IgA immune complexes prepared with monomeric or dimeric IgA. J Immunol. 1983 Apr;130(4):1826–1832. [PubMed] [Google Scholar]
  19. Rifai A., Mannik M. Clearance of circulating IgA immune complexes is mediated by a specific receptor on Kupffer cells in mice. J Exp Med. 1984 Jul 1;160(1):125–137. doi: 10.1084/jem.160.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rifai A., Millard K. Glomerular deposition of immune complexes prepared with monomeric or polymeric IgA. Clin Exp Immunol. 1985 May;60(2):363–368. [PMC free article] [PubMed] [Google Scholar]
  21. Sancho J., Egido J., González E. A simple method for determining polymeric IgA-containing immune complexes. J Immunol Methods. 1983 Jun 10;60(3):305–317. doi: 10.1016/0022-1759(83)90288-0. [DOI] [PubMed] [Google Scholar]
  22. Sancho J., Egido J., Rivera F., Hernando L. Immune complexes in IgA nephropathy: presence of antibodies against diet antigens and delayed clearance of specific polymeric IgA immune complexes. Clin Exp Immunol. 1983 Oct;54(1):194–202. [PMC free article] [PubMed] [Google Scholar]
  23. Sancho J., Egido J., Sánchez-Crespo M., Blasco R. Detection of monomeric and polymeric IgA containing immune complexes in serum and kidney from patients with alcoholic liver disease. Clin Exp Immunol. 1982 Feb;47(2):327–335. [PMC free article] [PubMed] [Google Scholar]
  24. Spalding D. M., Williamson S. I., Koopman W. J., McGhee J. R. Preferential induction of polyclonal IgA secretion by murine Peyer's patch dendritic cell-T cell mixtures. J Exp Med. 1984 Sep 1;160(3):941–946. doi: 10.1084/jem.160.3.941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tomino Y., Endoh M., Nomoto Y., Sakai H. Specificity of eluted antibody from renal tissues of patients with IgA nephropathy. Am J Kidney Dis. 1982 Mar;1(5):276–280. doi: 10.1016/s0272-6386(82)80025-5. [DOI] [PubMed] [Google Scholar]
  26. Trascasa M. L., Egido J., Sancho J., Hernando L. IgA glomerulonephritis (Berger's disease): evidence of high serum levels of polymeric IgA. Clin Exp Immunol. 1980 Nov;42(2):247–254. [PMC free article] [PubMed] [Google Scholar]
  27. Woodroffe A. J., Border W. A., Theofilopoulos A. N., Götze O., Glassock R. J., Dixon F. J., Wilson C. B. Detection of circulating immune complexes in patients with glomerulonephritis. Kidney Int. 1977 Oct;12(4):268–278. doi: 10.1038/ki.1977.111. [DOI] [PubMed] [Google Scholar]
  28. Woodroffe A. J., Gormly A. A., McKenzie P. E., Wootton A. M., Thompson A. J., Seymour A. E., Clarkson A. R. Immunologic studies in IgA nephropathy. Kidney Int. 1980 Sep;18(3):366–374. doi: 10.1038/ki.1980.147. [DOI] [PubMed] [Google Scholar]

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