Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1990 Aug;70(4):458–464.

Arachidonic acid metabolism in heat-shock treated human leucocytes.

M Köller 1, W König 1
PMCID: PMC1384249  PMID: 2118477

Abstract

Human neutrophil granulocyte fractions (PMN) and lymphocytes/monocytes/basophils (LMB) were stimulated with A23187 (7.3 microM), opsonized zymosan (1 mg) or FMLP (10(-5) M) after heat-shock treatment. We observed a temperature- (pretreatment over 40 degrees) and time-dependent (incubation periods longer than 20 min) suppression in the generation of LTB4, LTB4 metabolites and isomers, as well as LTC4 and 5-HETE. These effects were not reversed after the addition of exogenous arachidonic acid (AA;50 microM). In contrast, heat-shock treatment alone triggered platelets to generate 12-HETE. After 1 hr at 42 degrees, 135 +/- 24 ng of 12-HETE were generated from 1 x 10(8) cells. The 12-HETE generation was not dependent on extracellular Ca2+. Conversion of 14C-AA (2 nmol) revealed an enhanced metabolism of AA to 12-HETE by platelets after heat-shock treatment without exogenous Ca2+. PMN and LMB labelled with 35S-methionine led to heat-shock protein (HSP; 65,000, 83,000 MW) expression after heat-shock treatment at 42 degrees or in the presence of NDGA (1 x 10(-5) M) at 37 degrees. These results suggest a regulatory interaction between the generation of lipo-oxygenase products, cellular stress responses and the expression of HSP.

Full text

PDF
458

Images in this article

462Figure 4
on p.462
462Figure 5
on p.462

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bigby T. D., Meslier N. Transcellular lipoxygenase metabolism between monocytes and platelets. J Immunol. 1989 Sep 15;143(6):1948–1954. [PubMed] [Google Scholar]
  2. Binart N., Chambraud B., Dumas B., Rowlands D. A., Bigogne C., Levin J. M., Garnier J., Baulieu E. E., Catelli M. G. The cDNA-derived amino acid sequence of chick heat shock protein Mr 90,000 (HSP 90) reveals a "DNA like" structure: potential site of interaction with steroid receptors. Biochem Biophys Res Commun. 1989 Feb 28;159(1):140–147. doi: 10.1016/0006-291x(89)92415-7. [DOI] [PubMed] [Google Scholar]
  3. Blackwell G. J., Flower R. J. Inhibition of phospholipase. Br Med Bull. 1983 Jul;39(3):260–264. doi: 10.1093/oxfordjournals.bmb.a071830. [DOI] [PubMed] [Google Scholar]
  4. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  5. Calderwood S. K., Bornstein B., Farnum E. K., Stevenson M. A. Heat shock stimulates the release of arachidonic acid and the synthesis of prostaglandins and leukotriene B4 in mammalian cells. J Cell Physiol. 1989 Nov;141(2):325–333. doi: 10.1002/jcp.1041410214. [DOI] [PubMed] [Google Scholar]
  6. Craig E. A. The heat shock response. CRC Crit Rev Biochem. 1985;18(3):239–280. doi: 10.3109/10409238509085135. [DOI] [PubMed] [Google Scholar]
  7. Croset M., Lagarde M. Stereospecific inhibition of PGH2-induced platelet aggregation by lipoxygenase products of icosaenoic acids. Biochem Biophys Res Commun. 1983 May 16;112(3):878–883. doi: 10.1016/0006-291x(83)91699-6. [DOI] [PubMed] [Google Scholar]
  8. Demling R. H. Burns. N Engl J Med. 1985 Nov 28;313(22):1389–1398. doi: 10.1056/NEJM198511283132205. [DOI] [PubMed] [Google Scholar]
  9. Denis M., Gustafsson J. A., Wikström A. C. Interaction of the Mr = 90,000 heat shock protein with the steroid-binding domain of the glucocorticoid receptor. J Biol Chem. 1988 Dec 5;263(34):18520–18523. [PubMed] [Google Scholar]
  10. Eid N. S., Kravath R. E., Lanks K. W. Heat-shock protein synthesis by human polymorphonuclear cells. J Exp Med. 1987 May 1;165(5):1448–1452. doi: 10.1084/jem.165.5.1448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ferris D. K., Harel-Bellan A., Morimoto R. I., Welch W. J., Farrar W. L. Mitogen and lymphokine stimulation of heat shock proteins in T lymphocytes. Proc Natl Acad Sci U S A. 1988 Jun;85(11):3850–3854. doi: 10.1073/pnas.85.11.3850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goodwin J. S., Atluru D., Sierakowski S., Lianos E. A. Mechanism of action of glucocorticosteroids. Inhibition of T cell proliferation and interleukin 2 production by hydrocortisone is reversed by leukotriene B4. J Clin Invest. 1986 Apr;77(4):1244–1250. doi: 10.1172/JCI112427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Haire R. N., Peterson M. S., O'Leary J. J. Mitogen activation induces the enhanced synthesis of two heat-shock proteins in human lymphocytes. J Cell Biol. 1988 Mar;106(3):883–891. doi: 10.1083/jcb.106.3.883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Johnson H. M., Russell J. K., Torres B. A. Structural basis for arachidonic acid second messenger signal in gamma-interferon induction. Ann N Y Acad Sci. 1988;524:208–217. doi: 10.1111/j.1749-6632.1988.tb38544.x. [DOI] [PubMed] [Google Scholar]
  15. Köller M., König W., Brom J., Erbs G., Müller F. E. Studies on the mechanisms of granulocyte dysfunctions in severely burned patients--evidence for altered leukotriene generation. J Trauma. 1989 Apr;29(4):435–445. doi: 10.1097/00005373-198904000-00004. [DOI] [PubMed] [Google Scholar]
  16. Köller M., König W., Brom J., Raulf M., Gross-Weege W., Erbs G., Müller F. E. Generation of leukotrienes from human polymorphonuclear granulocytes of severely burned patients. J Trauma. 1988 Jun;28(6):733–740. doi: 10.1097/00005373-198806000-00002. [DOI] [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. Lefebvre P., Sablonniere B., Tbarka N., Formstecher P., Dautrevaux M. Study of the heteromeric structure of the untransformed glucocorticoid receptor using chemical cross-linking and monoclonal antibodies against the 90K heat-shock protein. Biochem Biophys Res Commun. 1989 Mar 15;159(2):677–686. doi: 10.1016/0006-291x(89)90048-x. [DOI] [PubMed] [Google Scholar]
  19. Lefer A. M. Leukotrienes as mediators of ischemia and shock. Biochem Pharmacol. 1986 Jan 15;35(2):123–127. doi: 10.1016/0006-2952(86)90502-2. [DOI] [PubMed] [Google Scholar]
  20. Lindquist S. The heat-shock response. Annu Rev Biochem. 1986;55:1151–1191. doi: 10.1146/annurev.bi.55.070186.005443. [DOI] [PubMed] [Google Scholar]
  21. Marcus A. J., Safier L. B., Ullman H. L., Islam N., Broekman M. J., von Schacky C. Studies on the mechanism of omega-hydroxylation of platelet 12-hydroxyeicosatetraenoic acid (12-HETE) by unstimulated neutrophils. J Clin Invest. 1987 Jan;79(1):179–187. doi: 10.1172/JCI112781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Maridonneau-Parini I., Clerc J., Polla B. S. Heat shock inhibits NADPH oxidase in human neutrophils. Biochem Biophys Res Commun. 1988 Jul 15;154(1):179–186. doi: 10.1016/0006-291x(88)90667-5. [DOI] [PubMed] [Google Scholar]
  23. Ohno K., Fukushima M., Fujiwara M., Narumiya S. Induction of 68,000-dalton heat shock proteins by cyclopentenone prostaglandins. Its association with prostaglandin-induced G1 block in cell cycle progression. J Biol Chem. 1988 Dec 25;263(36):19764–19770. [PubMed] [Google Scholar]
  24. Ottenhoff T. H., Ab B. K., Van Embden J. D., Thole J. E., Kiessling R. The recombinant 65-kD heat shock protein of Mycobacterium bovis Bacillus Calmette-Guerin/M. tuberculosis is a target molecule for CD4+ cytotoxic T lymphocytes that lyse human monocytes. J Exp Med. 1988 Nov 1;168(5):1947–1952. doi: 10.1084/jem.168.5.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Parker C. W. Lipid mediators produced through the lipoxygenase pathway. Annu Rev Immunol. 1987;5:65–84. doi: 10.1146/annurev.iy.05.040187.000433. [DOI] [PubMed] [Google Scholar]
  26. Rola-Pleszczynski M., Bouvrette L., Gingras D., Girard M. Identification of interferon-gamma as the lymphokine that mediates leukotriene B4-induced immunoregulation. J Immunol. 1987 Jul 15;139(2):513–517. [PubMed] [Google Scholar]
  27. Rola-Pleszczynski M., Gagnon L., Chavaillaz P. A. Immune regulation by leukotriene B4. Ann N Y Acad Sci. 1988;524:218–226. doi: 10.1111/j.1749-6632.1988.tb38545.x. [DOI] [PubMed] [Google Scholar]
  28. Russell J. K., Torres B. A., Johnson H. M. Phospholipase A2 treatment of lymphocytes provides helper signal for interferon-gamma induction. Evidence for second messenger role of endogenous arachidonic acid. J Immunol. 1987 Nov 15;139(10):3442–3446. [PubMed] [Google Scholar]
  29. Samuelsson B. Leukotrienes: mediators of immediate hypersensitivity reactions and inflammation. Science. 1983 May 6;220(4597):568–575. doi: 10.1126/science.6301011. [DOI] [PubMed] [Google Scholar]
  30. Santoro M. G., Garaci E., Amici C. Prostaglandins with antiproliferative activity induce the synthesis of a heat shock protein in human cells. Proc Natl Acad Sci U S A. 1989 Nov;86(21):8407–8411. doi: 10.1073/pnas.86.21.8407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Siess W. Molecular mechanisms of platelet activation. Physiol Rev. 1989 Jan;69(1):58–178. doi: 10.1152/physrev.1989.69.1.58. [DOI] [PubMed] [Google Scholar]
  32. Spector A. A., Gordon J. A., Moore S. A. Hydroxyeicosatetraenoic acids (HETEs). Prog Lipid Res. 1988;27(4):271–323. doi: 10.1016/0163-7827(88)90009-4. [DOI] [PubMed] [Google Scholar]
  33. Spector N. L., Freedman A. S., Freeman G., Segil J., Whitman J. F., Welch W. J., Nadler L. M. Activation primes human B lymphocytes to respond to heat shock. J Exp Med. 1989 Nov 1;170(5):1763–1768. doi: 10.1084/jem.170.5.1763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Yamaoka K. A., Claésson H. E., Rosén A. Leukotriene B4 enhances activation, proliferation, and differentiation of human B lymphocytes. J Immunol. 1989 Sep 15;143(6):1996–2000. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES