Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1990 Aug;70(4):504–512.

Molecular mimicry: a herpes virus glycoprotein antigenically related to a cell-surface glycoprotein expressed by macrophages, polymorphonuclear leucocytes, and platelets.

D R Fitzpatrick 1, M Snider 1, L McDougall 1, T Beskorwayne 1, L A Babiuk 1, T J Zamb 1, H Bielefeldt Ohmann 1
PMCID: PMC1384256  PMID: 1697564

Abstract

Bovine herpes virus type 1 (BHV-1) gIII is a major virion glycoprotein with homology to the immunoglobulin superfamily. We have investigated the possibility that gIII is related to host molecules and have identified a gIII-specific monoclonal antibody (mAb) that cross-reacts with normal bovine cells. The cross-reactive entity was expressed mainly on monocyte/macrophages (M phi), polymorphonuclear leucocytes (PMN) and platelets, and was identified as a 43,000-63,000 molecular weight (MW) cell-surface glycoprotein. For M phi, the glycoprotein appears to be a general lineage marker, rather than a maturation or activation marker, and may be a functional receptor, as evidenced by its endocytosis via coated pits and its involvement in proliferation of mononuclear cells in vitro. This novel leucocyte marker was also detected on subsets of human, ovine and canine M phi. Competitive binding assays with sera from cattle immunized with BHV-1 or gIII revealed apparent low responsiveness to the cross-reactive epitope. The results suggest that BHV-1 gIII is antigenically related to a novel host leucocyte receptor and that evasion and/or interference with leucocyte function may be a consequence of this molecular mimicry relationship.

Full text

PDF

Images in this article

506Figure 1
on p.506
507Figure 2
on p.507
509Figure 4
on p.509
510Figure 5
on p.510

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ayers V. K., Riegel C. A., Carman J., Collins J. K. Epitope specificity of the bovine antibody response to the gIII glycoprotein of bovine herpesvirus type 1. Viral Immunol. 1989;2(2):79–88. doi: 10.1089/vim.1989.2.79. [DOI] [PubMed] [Google Scholar]
  2. Babiuk L. A., Lawman M. J., Ohmann H. B. Viral-bacterial synergistic interaction in respiratory disease. Adv Virus Res. 1988;35:219–249. doi: 10.1016/S0065-3527(08)60713-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bielefeldt Ohmann H., Babiuk L. A. Bovine alveolar macrophages: phenotypic and functional properties of subpopulations obtained by Percoll density gradient centrifugation. J Leukoc Biol. 1986 Feb;39(2):167–181. doi: 10.1002/jlb.39.2.167. [DOI] [PubMed] [Google Scholar]
  4. Bielefeldt Ohmann H., Babiuk L. A. Viral-bacterial pneumonia in calves: effect of bovine herpesvirus-1 on immunologic functions. J Infect Dis. 1985 May;151(5):937–947. doi: 10.1093/infdis/151.5.937. [DOI] [PubMed] [Google Scholar]
  5. Bielefeldt-Ohmann H., Sabara M., Lawman M. J., Griebel P., Babiuk L. A. A monoclonal antibody detects macrophage maturation antigen which appears independently of class II antigen expression. Reactivity of monoclonal EBM11 with bovine macrophages. J Immunol. 1988 Apr 1;140(7):2201–2209. [PubMed] [Google Scholar]
  6. Damian R. T. Molecular mimicry: parasite evasion and host defense. Curr Top Microbiol Immunol. 1989;145:101–115. doi: 10.1007/978-3-642-74594-2_9. [DOI] [PubMed] [Google Scholar]
  7. Davis W. C., Marusic S., Lewin H. A., Splitter G. A., Perryman L. E., McGuire T. C., Gorham J. R. The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species. Vet Immunol Immunopathol. 1987 Jul;15(4):337–376. doi: 10.1016/0165-2427(87)90005-5. [DOI] [PubMed] [Google Scholar]
  8. Fitzpatrick D. R., Redmond M. J., Attah-Poku S. K., van Drunen Littel-van den Hurk S., Babiuk L. A., Zamb T. J. Mapping of 10 epitopes on bovine herpesvirus type 1 glycoproteins gI and gIII. Virology. 1990 May;176(1):145–157. doi: 10.1016/0042-6822(90)90239-n. [DOI] [PubMed] [Google Scholar]
  9. Fitzpatrick D. R., Zamb T., Parker M. D., van Drunen Littel-van den Hurk S., Babiuk L. A., Lawman M. J. Expression of bovine herpesvirus 1 glycoproteins gI and gIII in transfected murine cells. J Virol. 1988 Nov;62(11):4239–4248. doi: 10.1128/jvi.62.11.4239-4248.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Forman A. J., Babiuk L. A. Effect of infectious bovine rhinotracheitis virus infection on bovine alveolar macrophage function. Infect Immun. 1982 Mar;35(3):1041–1047. doi: 10.1128/iai.35.3.1041-1047.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fujinami R. S., Nelson J. A., Walker L., Oldstone M. B. Sequence homology and immunologic cross-reactivity of human cytomegalovirus with HLA-DR beta chain: a means for graft rejection and immunosuppression. J Virol. 1988 Jan;62(1):100–105. doi: 10.1128/jvi.62.1.100-105.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goyert S. M., Ferrero E. M., Seremetis S. V., Winchester R. J., Silver J., Mattison A. C. Biochemistry and expression of myelomonocytic antigens. J Immunol. 1986 Dec 15;137(12):3909–3914. [PubMed] [Google Scholar]
  13. Griebel P. J., Ohmann H. B., Lawman M. J., Babiuk L. A. The interaction between bovine herpesvirus type 1 and activated bovine T lymphocytes. J Gen Virol. 1990 Feb;71(Pt 2):369–377. doi: 10.1099/0022-1317-71-2-369. [DOI] [PubMed] [Google Scholar]
  14. Horejsí V., Bazil V. Surface proteins and glycoproteins of human leucocytes. Biochem J. 1988 Jul 1;253(1):1–26. doi: 10.1042/bj2530001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hubbard A. L. Endocytosis. Curr Opin Cell Biol. 1989 Aug;1(4):675–683. doi: 10.1016/0955-0674(89)90033-1. [DOI] [PubMed] [Google Scholar]
  16. Israel B. A., Marshall R. L., Letchworth G. J., 3rd Epitope specificity and protective efficacy of the bovine immune response to bovine herpesvirus-1 glycoprotein vaccines. Vaccine. 1988 Aug;6(4):349–356. doi: 10.1016/0264-410x(88)90182-x. [DOI] [PubMed] [Google Scholar]
  17. Knapp W., Rieber P., Dörken B., Schmidt R. E., Stein H., vd Borne A. E. Towards a better definition of human leucocyte surface molecules. Immunol Today. 1989 Aug;10(8):253–258. doi: 10.1016/0167-5699(89)90135-7. [DOI] [PubMed] [Google Scholar]
  18. McKinney M. M., Parkinson A. A simple, non-chromatographic procedure to purify immunoglobulins from serum and ascites fluid. J Immunol Methods. 1987 Feb 11;96(2):271–278. doi: 10.1016/0022-1759(87)90324-3. [DOI] [PubMed] [Google Scholar]
  19. Misra V., Blumenthal R. M., Babiuk L. A. Proteins Specified by bovine herpesvirus 1 (infectious bovine rhinotracheitis virus). J Virol. 1981 Nov;40(2):367–378. doi: 10.1128/jvi.40.2.367-378.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nemerow G. R., Houghten R. A., Moore M. D., Cooper N. R. Identification of an epitope in the major envelope protein of Epstein-Barr virus that mediates viral binding to the B lymphocyte EBV receptor (CR2). Cell. 1989 Feb 10;56(3):369–377. doi: 10.1016/0092-8674(89)90240-7. [DOI] [PubMed] [Google Scholar]
  21. Ohmann H. B., Campos M., Lawman M. J., Babiuk L. A. Induction of MHC class II antigens on bovine cells of nonlymphoid origin by recombinant bovine interferon-gamma and tumor necrosis factor-alpha. J Interferon Res. 1988 Aug;8(4):451–462. doi: 10.1089/jir.1988.8.451. [DOI] [PubMed] [Google Scholar]
  22. Okazaki K., Honda E., Minetoma T., Kumagai T. Mechanisms of neutralization by monoclonal antibodies to different antigenic sites on the bovine herpesvirus type 1 glycoproteins. Virology. 1986 Apr 15;150(1):260–264. doi: 10.1016/0042-6822(86)90285-0. [DOI] [PubMed] [Google Scholar]
  23. van Drunen Littel-van den Hurk S., Babiuk L. A. Antigenic and immunogenic characteristics of bovine herpesvirus type-1 glycoproteins GVP 3/9 and GVP 6/11a/16, purified by immunoadsorbent chromatography. Virology. 1985 Jul 15;144(1):204–215. doi: 10.1016/0042-6822(85)90318-6. [DOI] [PubMed] [Google Scholar]
  24. van Drunen Littel-van den Hurk S., Babiuk L. A. Synthesis and processing of bovine herpesvirus 1 glycoproteins. J Virol. 1986 Aug;59(2):401–410. doi: 10.1128/jvi.59.2.401-410.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. van Drunen Littel-van den Hurk S., van den Hurk J. V., Gilchrist J. E., Misra V., Babiuk L. A. Interactions of monoclonal antibodies and bovine herpesvirus type 1 (BHV-1) glycoproteins: characterization of their biochemical and immunological properties. Virology. 1984 Jun;135(2):466–479. doi: 10.1016/0042-6822(84)90201-0. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES