Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1990 Aug;70(4):547–550.

A chymotryptic-type serine protease is required for IL-2 production by Jurkat T cells.

P Auberger 1, S Sonthonnax 1, J F Peyron 1, B Mari 1, M Fehlmann 1
PMCID: PMC1384262  PMID: 2394468

Abstract

Interleukin-2 (IL-2) production by activated Jurkat T cells was markedly delayed when these cells were treated with low concentrations of the chymotryptic-type protease inhibitor N-alpha-p-tosyl-L-phenylalanine chloromethylketone (TPCK). This increased lag time observed in the presence of TPCK directly correlates with the interaction of the inhibitor with a unique 42,000 molecular weight (MW) serine protease, which can be labelled with [3H]DFP, and was not due to an intracellular accumulation of a non-mature form of IL-2 nor to a non-specific inhibition of overall protein synthesis. The results presented in this report indicate that a 42,000 MW chymotryptic-like serine protease is required for IL-2 production by activated Jurkat T cells.

Full text

PDF
547

Images in this article

548Figure 2
on p.548

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auberger P., Mary D., Breittmayer J. P., Aussel C., Fehlmann M. Chymotryptic-type protease inhibitors block the increase in Ca2+ and Il-2 production in activated Jurkat T cells. J Immunol. 1989 Feb 15;142(4):1253–1259. [PubMed] [Google Scholar]
  2. Auberger P., Samson M., Le Cam G., Le Cam A. Effects of polyamines on cyclic AMP-mediated stimulation of amino acid transport in isolated rat hepatocytes. J Cell Physiol. 1983 Nov;117(2):204–210. doi: 10.1002/jcp.1041170211. [DOI] [PubMed] [Google Scholar]
  3. Davis L., Lipsky P. E. Signals involved in T cell activation. I. Phorbol esters enhance responsiveness but cannot replace intact accessory cells in the induction of mitogen-stimulated T cell proliferation. J Immunol. 1985 Nov;135(5):2946–2952. [PubMed] [Google Scholar]
  4. Didier M., Aussel C., Pelassy C., Fehlmann M. IL-1 signaling for IL-2 production in T cells involves a rise in phosphatidylserine synthesis. J Immunol. 1988 Nov 1;141(9):3078–3080. [PubMed] [Google Scholar]
  5. Ferrua B., Aussel C., Fehlmann M. Human interleukin 2. Detection at the picomolar level by sandwich enzyme immunoassay. J Immunol Methods. 1987 Mar 12;97(2):215–220. doi: 10.1016/0022-1759(87)90462-5. [DOI] [PubMed] [Google Scholar]
  6. Gutowski J. K., Mukherji B., Cohen S. The role of cytoplasmic intermediates in IL 2-induced T cell growth. J Immunol. 1984 Dec;133(6):3068–3074. [PubMed] [Google Scholar]
  7. Imboden J. B., Weiss A., Stobo J. D. The antigen receptor on a human T cell line initiates activation by increasing cytoplasmic free calcium. J Immunol. 1985 Feb;134(2):663–665. [PubMed] [Google Scholar]
  8. Kishimoto T., Kikutani H., Nishizawa Y., Sakaguchi N., Yamamura Y. Involvement of anti-Ig-activated serine protease in the generation of cytoplasmic factor(s) that are responsible for the transmission of Ig-receptor-mediated signals. J Immunol. 1979 Oct;123(4):1504–1510. [PubMed] [Google Scholar]
  9. Ku G. S., Quigley J. P., Sultzer B. M. The inhibition of the mitogenic stimulation of B lymphocytes by a serine protease inhibitor: commitment to proliferation correlates with an enhanced expression of a cell-associated arginine-specific serine enzyme. J Immunol. 1983 Nov;131(5):2494–2499. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Manger B., Weiss A., Weyand C., Goronzy J., Stobo J. D. T cell activation: differences in the signals required for IL 2 production by nonactivated and activated T cells. J Immunol. 1985 Dec;135(6):3669–3673. [PubMed] [Google Scholar]
  12. Schön E., Jahn S., Kiessig S. T., Demuth H. U., Neubert K., Barth A., Von Baehr R., Ansorge S. The role of dipeptidyl peptidase IV in human T lymphocyte activation. Inhibitors and antibodies against dipeptidyl peptidase IV suppress lymphocyte proliferation and immunoglobulin synthesis in vitro. Eur J Immunol. 1987 Dec;17(12):1821–1826. doi: 10.1002/eji.1830171222. [DOI] [PubMed] [Google Scholar]
  13. Weiss A., Imboden J., Shoback D., Stobo J. Role of T3 surface molecules in human T-cell activation: T3-dependent activation results in an increase in cytoplasmic free calcium. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4169–4173. doi: 10.1073/pnas.81.13.4169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wong R. L., Gutowski J. K., Katz M., Goldfarb R. H., Cohen S. Induction of DNA synthesis in isolated nuclei by cytoplasmic factors: inhibition by protease inhibitors. Proc Natl Acad Sci U S A. 1987 Jan;84(1):241–245. doi: 10.1073/pnas.84.1.241. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES