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. 1996 Feb;87(2):334–338. doi: 10.1046/j.1365-2567.1996.463529.x

Analysis of immunoglobulin variable region genes of a human IgM anti-myeloperoxidase antibody derived from a patient with vasculitis.

C Longhurst 1, M R Ehrenstein 1, B Leaker 1, F K Stevenson 1, M Spellerberg 1, C Chapman 1, D Latchmen 1, D A Isenberg 1, G Cambridge 1
PMCID: PMC1384293  PMID: 8698399

Abstract

Circulating antibodies to myeloperoxidase (MPO) are associated primarily with pauci-immune glomerulonephritis and systemic vasculitis. Anti-MPO antibodies belong to a group of autoantibodies, anti-neutrophil cytoplasmic antibodies, that may play a pathogenic role in vasculitis. We have generated a human monoclonal anti-MPO antibody (E3-MPO) using peripheral blood lymphocytes from a patient with microscopic polyarteritis. Variable region gene analysis of E3-MPO showed that the VH region had 90% homology with the germ line gene VH4-21. E3-MPO was also shown to carry the 9G4 idiotope, which so far has been associated only with human antibodies that utilize the VH4-21 gene. The 9G4 idiotope was also expressed on anti-MPO antibodies in sera from the donor patient and from 4/7 additional patients with active, untreated vasculitis. The nucleotide sequences of both the variable heavy and light chains of E3-MPO showed evidence of an antigen-driven response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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