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. 1991 Jan;72(1):10–14.

MHC class II-restricted T-cell hybridomas recognizing the nucleocapsid protein of avian coronavirus IBV.

A M Boots 1, M J Van Lierop 1, J G Kusters 1, P J Van Kooten 1, B A Van der Zeijst 1, E J Hensen 1
PMCID: PMC1384328  PMID: 1847691

Abstract

Mice were immunized with purified infectious bronchitis virus (IBV), strain M41. Spleen cells, expanded in vitro by stimulation with M41, were immortalized by fusion to obtain T-cell hybridomas, and two major histocompatability complex (MHC) class II (I-E)-restricted T-cell hybridomas were selected with specificity for IBV. Both hybridomas selectively recognized the internal nucleocapsid protein. The responses to 12 different strains of IBV varied markedly. This demonstrates antigenic variation of the nucleocapsid protein in addition to the known variation of the surface glycoprotein S.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boursnell M. E., Binns M. M., Foulds I. J., Brown T. D. Sequences of the nucleocapsid genes from two strains of avian infectious bronchitis virus. J Gen Virol. 1985 Mar;66(Pt 3):573–580. doi: 10.1099/0022-1317-66-3-573. [DOI] [PubMed] [Google Scholar]
  2. Cavanagh D. Coronavirus IBV glycopolypeptides: size of their polypeptide moieties and nature of their oligosaccharides. J Gen Virol. 1983 May;64(Pt 5):1187–1191. doi: 10.1099/0022-1317-64-5-1187. [DOI] [PubMed] [Google Scholar]
  3. Cavanagh D. Coronavirus IBV: further evidence that the surface projections are associated with two glycopolypeptides. J Gen Virol. 1983 Aug;64(Pt 8):1787–1791. doi: 10.1099/0022-1317-64-8-1787. [DOI] [PubMed] [Google Scholar]
  4. Curling E., Slade C., Hutchinson I., Morris P. Isolation of rat/rat T cell hybrids using W/Fu C58(NT)D as fusion partner. J Immunol Methods. 1988 Apr 6;108(1-2):171–178. doi: 10.1016/0022-1759(88)90416-4. [DOI] [PubMed] [Google Scholar]
  5. Davelaar F. G., Kouwenhoven B., Burger A. G. Occurrence and significance of infectious bronchitis virus variant strains in egg and broiler production in the Netherlands. Vet Q. 1984 Jul;6(3):114–120. doi: 10.1080/01652176.1984.9693924. [DOI] [PubMed] [Google Scholar]
  6. Dietzschold B., Wang H. H., Rupprecht C. E., Celis E., Tollis M., Ertl H., Heber-Katz E., Koprowski H. Induction of protective immunity against rabies by immunization with rabies virus ribonucleoprotein. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9165–9169. doi: 10.1073/pnas.84.24.9165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Germain R. N., Ashwell J. D., Lechler R. I., Margulies D. H., Nickerson K. M., Suzuki G., Tou J. Y. "Exon-shuffling" maps control of antibody- and T-cell-recognition sites to the NH2-terminal domain of the class II major histocompatibility polypeptide A beta. Proc Natl Acad Sci U S A. 1985 May;82(9):2940–2944. doi: 10.1073/pnas.82.9.2940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Germain R. N., Quill H. Unexpected expression of a unique mixed-isotype class II MHC molecule by transfected L-cells. Nature. 1986 Mar 6;320(6057):72–75. doi: 10.1038/320072a0. [DOI] [PubMed] [Google Scholar]
  9. Gillis S., Ferm M. M., Ou W., Smith K. A. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed] [Google Scholar]
  10. Jacobs L., Spaan W. J., Horzinek M. C., van der Zeijst B. A. Synthesis of subgenomic mRNA's of mouse hepatitis virus is initiated independently: evidence from UV transcription mapping. J Virol. 1981 Aug;39(2):401–406. doi: 10.1128/jvi.39.2.401-406.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kast W. M., de Waal L. P., Melief C. J. Thymus dictates major histocompatibility complex (MHC) specificity and immune response gene phenotype of class II MHC-restricted T cells but not of class I MHC-restricted T cells. J Exp Med. 1984 Dec 1;160(6):1752–1766. doi: 10.1084/jem.160.6.1752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kusters J. G., Jager E. J., Lenstra J. A., Koch G., Posthumus W. P., Meloen R. H., van der Zeijst B. A. Analysis of an immunodominant region of infectious bronchitis virus. J Immunol. 1989 Oct 15;143(8):2692–2698. [PubMed] [Google Scholar]
  13. Kusters J. G., Niesters H. G., Bleumink-Pluym N. M., Davelaar F. G., Horzinek M. C., Van der Zeijst B. A. Molecular epidemiology of infectious bronchitis virus in The Netherlands. J Gen Virol. 1987 Feb;68(Pt 2):343–352. doi: 10.1099/0022-1317-68-2-343. [DOI] [PubMed] [Google Scholar]
  14. Kusters J. G., Niesters H. G., Lenstra J. A., Horzinek M. C., van der Zeijst B. A. Phylogeny of antigenic variants of avian coronavirus IBV. Virology. 1989 Mar;169(1):217–221. doi: 10.1016/0042-6822(89)90058-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lamb J. R., Woody J. N., Hartzman R. J., Eckels D. D. In vitro influenza virus-specific antibody production in man: antigen-specific and HLA-restricted induction of helper activity mediated by cloned human T lymphocytes. J Immunol. 1982 Oct;129(4):1465–1470. [PubMed] [Google Scholar]
  16. Lassila O., Vainio O., Matzinger P. Can B cells turn on virgin T cells? Nature. 1988 Jul 21;334(6179):253–255. doi: 10.1038/334253a0. [DOI] [PubMed] [Google Scholar]
  17. Lecomte J., Cainelli-Gebara V., Mercier G., Mansour S., Talbot P. J., Lussier G., Oth D. Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody. Brief report. Arch Virol. 1987;97(1-2):123–130. doi: 10.1007/BF01310740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lenstra J. A., Kusters J. G., Koch G., van der Zeijst B. A. Antigenicity of the peplomer protein of infectious bronchitis virus. Mol Immunol. 1989 Jan;26(1):7–15. doi: 10.1016/0161-5890(89)90014-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Milich D. R., McLachlan A., Thornton G. B., Hughes J. L. Antibody production to the nucleocapsid and envelope of the hepatitis B virus primed by a single synthetic T cell site. Nature. 1987 Oct 8;329(6139):547–549. doi: 10.1038/329547a0. [DOI] [PubMed] [Google Scholar]
  20. Nakanaga K., Yamanouchi K., Fujiwara K. Protective effect of monoclonal antibodies on lethal mouse hepatitis virus infection in mice. J Virol. 1986 Jul;59(1):168–171. doi: 10.1128/jvi.59.1.168-171.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Niesters H. G., Lenstra J. A., Spaan W. J., Zijderveld A. J., Bleumink-Pluym N. M., Hong F., van Scharrenburg G. J., Horzinek M. C., van der Zeijst B. A. The peplomer protein sequence of the M41 strain of coronavirus IBV and its comparison with Beaudette strains. Virus Res. 1986 Aug;5(2-3):253–263. doi: 10.1016/0168-1702(86)90022-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ronchese F., Brown M. A., Germain R. N. Structure-function analysis of the Abm12 beta mutation using site-directed mutagenesis and DNA-mediated gene transfer. J Immunol. 1987 Jul 15;139(2):629–638. [PubMed] [Google Scholar]
  23. Russell S. M., Liew F. Y. T cells primed by influenza virion internal components can cooperate in the antibody response to haemagglutinin. Nature. 1979 Jul 12;280(5718):147–148. doi: 10.1038/280147a0. [DOI] [PubMed] [Google Scholar]
  24. Scherle P. A., Gerhard W. Differential ability of B cells specific for external vs. internal influenza virus proteins to respond to help from influenza virus-specific T-cell clones in vivo. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4446–4450. doi: 10.1073/pnas.85.12.4446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Silva A., MacDonald H. R., Conzelmann A., Corthésy P., Nabholz M. Rat X mouse T-cell hybrids with inducible specific cytolytic activity. Immunol Rev. 1983;76:105–129. doi: 10.1111/j.1600-065x.1983.tb01099.x. [DOI] [PubMed] [Google Scholar]
  26. Stern D. F., Sefton B. M. Coronavirus multiplication: locations of genes for virion proteins on the avian infectious bronchitis virus genome. J Virol. 1984 Apr;50(1):22–29. doi: 10.1128/jvi.50.1.22-29.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sturman L. S., Holmes K. V., Behnke J. Isolation of coronavirus envelope glycoproteins and interaction with the viral nucleocapsid. J Virol. 1980 Jan;33(1):449–462. doi: 10.1128/jvi.33.1.449-462.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Townsend A. R., McMichael A. J., Carter N. P., Huddleston J. A., Brownlee G. G. Cytotoxic T cell recognition of the influenza nucleoprotein and hemagglutinin expressed in transfected mouse L cells. Cell. 1984 Nov;39(1):13–25. doi: 10.1016/0092-8674(84)90187-9. [DOI] [PubMed] [Google Scholar]

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