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. 1991 Jan;72(1):61–64.

Regulation of interleukin-1 and tumour necrosis factor gene expression in myelomonocytic cell lines by 1,25-dihydroxyvitamin D3.

A K Bhalla 1, T Paavonen 1, M M Williams 1, P J Delves 1, P M Lydyard 1
PMCID: PMC1384336  PMID: 1997401

Abstract

1,25-dihydroxyvitamin D3[1,25-(OH)2D3] is capable of regulating cells in the immune system and affects cytokine production by both T lymphocytes and by monocytes. We examined the effects of 1,25-(OH)2D3 on the regulation of interleukin-1 beta (IL-1 beta) and tumour necrosis factor-alpha (TNF-alpha) genes in HL-60 and U937 cells. 1,25-(OH)2D3 alone only induced low level expression of the genes for these cytokines. Phorbol 12-myristate 13-acetate (PMA) strongly induced the transcription of these genes, whilst the addition of 1,25-(OH)2D3 to PMA-stimulated cells caused a further dose-dependent synergistic increase in the mRNA for both cytokines in U937 cells. In PMA-stimulated HL-60 cells, 1,25-(OH)2D3 increased the mRNA for IL-1 beta but not that for TNA-alpha, These differences may be related to the different stage of myeloid differentiation in HL-60 and U937 cells.

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  1. Adams J. S., Sharma O. P., Gacad M. A., Singer F. R. Metabolism of 25-hydroxyvitamin D3 by cultured pulmonary alveolar macrophages in sarcoidosis. J Clin Invest. 1983 Nov;72(5):1856–1860. doi: 10.1172/JCI111147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Amento E. P., Bhalla A. K., Kurnick J. T., Kradin R. L., Clemens T. L., Holick S. A., Holick M. F., Krane S. M. 1 alpha,25-dihydroxyvitamin D3 induces maturation of the human monocyte cell line U937, and, in association with a factor from human T lymphocytes, augments production of the monokine, mononuclear cell factor. J Clin Invest. 1984 Mar;73(3):731–739. doi: 10.1172/JCI111266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bar-Shavit Z., Teitelbaum S. L., Reitsma P., Hall A., Pegg L. E., Trial J., Kahn A. J. Induction of monocytic differentiation and bone resorption by 1,25-dihydroxyvitamin D3. Proc Natl Acad Sci U S A. 1983 Oct;80(19):5907–5911. doi: 10.1073/pnas.80.19.5907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bhalla A. K., Amento E. P., Clemens T. L., Holick M. F., Krane S. M. Specific high-affinity receptors for 1,25-dihydroxyvitamin D3 in human peripheral blood mononuclear cells: presence in monocytes and induction in T lymphocytes following activation. J Clin Endocrinol Metab. 1983 Dec;57(6):1308–1310. doi: 10.1210/jcem-57-6-1308. [DOI] [PubMed] [Google Scholar]
  5. Bhalla A. K., Amento E. P., Krane S. M. Differential effects of 1,25-dihydroxyvitamin D3 on human lymphocytes and monocyte/macrophages: inhibition of interleukin-2 and augmentation of interleukin-1 production. Cell Immunol. 1986 Apr 1;98(2):311–322. doi: 10.1016/0008-8749(86)90291-1. [DOI] [PubMed] [Google Scholar]
  6. Bhalla A. K., Williams M. M., Lal S., Lydyard P. M. 1,25-Dihydroxyvitamin D3, but not retinoic acid, induces the differentiation of U937 cells. Clin Exp Immunol. 1989 May;76(2):274–277. [PMC free article] [PubMed] [Google Scholar]
  7. Breitman T. R., Selonick S. E., Collins S. J. Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid. Proc Natl Acad Sci U S A. 1980 May;77(5):2936–2940. doi: 10.1073/pnas.77.5.2936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  9. Cohen M. S., Mesler D. E., Snipes R. G., Gray T. K. 1,25-Dihydroxyvitamin D3 activates secretion of hydrogen peroxide by human monocytes. J Immunol. 1986 Feb 1;136(3):1049–1053. [PubMed] [Google Scholar]
  10. Fenton M. J., Vermeulen M. W., Clark B. D., Webb A. C., Auron P. E. Human pro-IL-1 beta gene expression in monocytic cells is regulated by two distinct pathways. J Immunol. 1988 Apr 1;140(7):2267–2273. [PubMed] [Google Scholar]
  11. Kioussis D., Wilson F., Daniels C., Leveton C., Taverne J., Playfair J. H. Expression and rescuing of a cloned human tumour necrosis factor gene using an EBV-based shuttle cosmid vector. EMBO J. 1987 Feb;6(2):355–361. doi: 10.1002/j.1460-2075.1987.tb04762.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Koeffler H. P. Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications. Blood. 1983 Oct;62(4):709–721. [PubMed] [Google Scholar]
  13. Lomedico P. T., Kilian P. L., Gubler U., Stern A. S., Chizzonite R. Molecular biology of interleukin-1. Cold Spring Harb Symp Quant Biol. 1986;51(Pt 1):631–639. doi: 10.1101/sqb.1986.051.01.075. [DOI] [PubMed] [Google Scholar]
  14. Olsson I. L., Breitman T. R., Gallo R. C. Priming of human myeloid leukemic cell lines HL-60 and U-937 with retinoic acid for differentiation effects of cyclic adenosine 3':5'-monophosphate-inducing agents and a T-lymphocyte-derived differentiation factor. Cancer Res. 1982 Oct;42(10):3928–3933. [PubMed] [Google Scholar]
  15. Reichel H., Koeffler H. P., Tobler A., Norman A. W. 1 alpha,25-Dihydroxyvitamin D3 inhibits gamma-interferon synthesis by normal human peripheral blood lymphocytes. Proc Natl Acad Sci U S A. 1987 May;84(10):3385–3389. doi: 10.1073/pnas.84.10.3385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rigby W. F., Shen L., Ball E. D., Guyre P. M., Fanger M. W. Differentiation of a human monocytic cell line by 1,25-dihydroxyvitamin D3 (calcitriol): a morphologic, phenotypic, and functional analysis. Blood. 1984 Nov;64(5):1110–1115. [PubMed] [Google Scholar]
  17. Rook G. A., Steele J., Fraher L., Barker S., Karmali R., O'Riordan J., Stanford J. Vitamin D3, gamma interferon, and control of proliferation of Mycobacterium tuberculosis by human monocytes. Immunology. 1986 Jan;57(1):159–163. [PMC free article] [PubMed] [Google Scholar]
  18. Steffen M., Cayre Y., Manogue K. R., Moore M. A. 1,25-Dihydroxyvitamin D3 transcriptionally regulates tumour necrosis factor mRNA during HL-60 cell differentiation. Immunology. 1988 Jan;63(1):43–46. [PMC free article] [PubMed] [Google Scholar]
  19. Tobler A., Miller C. W., Norman A. W., Koeffler H. P. 1,25-Dihydroxyvitamin D3 modulates the expression of a lymphokine (granulocyte-macrophage colony-stimulating factor) posttranscriptionally. J Clin Invest. 1988 Jun;81(6):1819–1823. doi: 10.1172/JCI113525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tsoukas C. D., Provvedini D. M., Manolagas S. C. 1,25-dihydroxyvitamin D3: a novel immunoregulatory hormone. Science. 1984 Jun 29;224(4656):1438–1440. doi: 10.1126/science.6427926. [DOI] [PubMed] [Google Scholar]
  21. Ucla C., Roux-Lombard P., Fey S., Dayer J. M., Mach B. Interferon gamma drastically modifies the regulation of interleukin 1 genes by endotoxin in U937 cells. J Clin Invest. 1990 Jan;85(1):185–191. doi: 10.1172/JCI114411. [DOI] [PMC free article] [PubMed] [Google Scholar]

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