Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1991 Mar;72(3):368–372.

Establishment of immortalized cell lines from mouse peritoneal macrophages following transformation with SV40 early region DNA deleted at the origin of replication.

U Kreuzburg-Duffy 1, C Macdonald 1
PMCID: PMC1384397  PMID: 1851133

Abstract

Two murine peritoneal macrophage cell lines have been isolated by transforming primary cells with simian virus 40 (SV40) origin-deleted DNA. These lines have been maintained in continuous culture for over 8 months and have been shown to express macrophage-specific properties throughout this time. The cell lines are F4/80 positive; express Fc receptors; will phagocytose immunoglobulin-coated red cells and latex beads; stain with neutral red; and have non-specific esterase and plasminogen activator activities. Lysozyme, collagenase, prostaglandin E2, acid phosphatase and 5'-nucleotidase activities have also been detected and quantified.

Full text

PDF
368

Images in this article

370Figure 1
on p.370
371Figure 2
on p.371

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austyn J. M., Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur J Immunol. 1981 Oct;11(10):805–815. doi: 10.1002/eji.1830111013. [DOI] [PubMed] [Google Scholar]
  2. Blasi E., Mathieson B. J., Varesio L., Cleveland J. L., Borchert P. A., Rapp U. R. Selective immortalization of murine macrophages from fresh bone marrow by a raf/myc recombinant murine retrovirus. Nature. 1985 Dec 19;318(6047):667–670. doi: 10.1038/318667a0. [DOI] [PubMed] [Google Scholar]
  3. Blasi E., Radzioch D., Durum S. K., Varesio L. A murine macrophage cell line, immortalized by v-raf and v-myc oncogenes, exhibits normal macrophage functions. Eur J Immunol. 1987 Oct;17(10):1491–1498. doi: 10.1002/eji.1830171016. [DOI] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. Cahoon B. E., Mills J. Development of characterization of a continuous line of alveolar macrophages from C3H/HEJ mice. J Reticuloendothel Soc. 1981 May;29(5):357–367. [PubMed] [Google Scholar]
  6. Gallimore P. H., Grand R. J., Byrd P. J. Transformation of human embryo retinoblasts with simian virus 40, adenovirus and ras oncogenes. Anticancer Res. 1986 May-Jun;6(3 Pt B):499–508. [PubMed] [Google Scholar]
  7. Gluzman Y., Sambrook J. F., Frisque R. J. Expression of early genes of origin-defective mutants of simian virus 40. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3898–3902. doi: 10.1073/pnas.77.7.3898. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gorman C., Padmanabhan R., Howard B. H. High efficiency DNA-mediated transformation of primate cells. Science. 1983 Aug 5;221(4610):551–553. doi: 10.1126/science.6306768. [DOI] [PubMed] [Google Scholar]
  9. Lombard Y., Bartholeyns J., Chokri M., Illinger D., Hartmann D., Dumont S., Kaufmann S. H., Landmann R., Loor F., Poindron P. Establishment and characterization of long-term cultured cell lines of murine resident macrophages. J Leukoc Biol. 1988 Nov;44(5):391–401. doi: 10.1002/jlb.44.5.391. [DOI] [PubMed] [Google Scholar]
  10. MacDonald C. Development of new cell lines for animal cell biotechnology. Crit Rev Biotechnol. 1990;10(2):155–178. doi: 10.3109/07388559009068265. [DOI] [PubMed] [Google Scholar]
  11. Mauel J., Defendi V. Infection and transformation of mouse peritoneal macrophages by simian virus 40. J Exp Med. 1971 Aug 1;134(2):335–350. doi: 10.1084/jem.134.2.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mayne L. V., Priestley A., James M. R., Burke J. F. Efficient immortalization and morphological transformation of human fibroblasts by transfection with SV40 DNA linked to a dominant marker. Exp Cell Res. 1986 Feb;162(2):530–538. doi: 10.1016/0014-4827(86)90356-3. [DOI] [PubMed] [Google Scholar]
  13. Mole S. E., Gannon J. V., Ford M. J., Lane D. P. Structure and function of SV40 large-T antigen. Philos Trans R Soc Lond B Biol Sci. 1987 Dec 15;317(1187):455–469. doi: 10.1098/rstb.1987.0072. [DOI] [PubMed] [Google Scholar]
  14. Nagata Y., Diamond B., Bloom B. R. The generation of human monocyte/macrophage cell lines. Nature. 1983 Dec 8;306(5943):597–599. doi: 10.1038/306597a0. [DOI] [PubMed] [Google Scholar]
  15. Revoltella R. P., Vigneti E., Ragona G., Rocchi G. Human monocytic cell lines derived from cord leukocytes by co-cultivation with irradiated CM-S cells. Science. 1984 Jun 8;224(4653):1124–1127. doi: 10.1126/science.6719138. [DOI] [PubMed] [Google Scholar]
  16. Schwarzbaum S., Halpern R., Diamond B. The generation of macrophage-like cell lines by transfection with SV40 origin defective DNA. J Immunol. 1984 Mar;132(3):1158–1162. [PubMed] [Google Scholar]
  17. Scott D. M., MacDonald C., Brzeski H., Kinne R. Maintenance of expression of differentiated function of kidney cells following transformation by SV40 early region DNA. Exp Cell Res. 1986 Oct;166(2):391–398. doi: 10.1016/0014-4827(86)90485-4. [DOI] [PubMed] [Google Scholar]
  18. Setoguchi M., Yoshida S., Higuchi Y., Akizuki S., Yamamoto S. Molecular analysis of expression of parental cell properties in hybrids between monocytes and a myeloma cell line. Somat Cell Mol Genet. 1988 Sep;14(5):427–438. doi: 10.1007/BF01534710. [DOI] [PubMed] [Google Scholar]
  19. Small M. B., Gluzman Y., Ozer H. L. Enhanced transformation of human fibroblasts by origin-defective simian virus 40. Nature. 1982 Apr 15;296(5858):671–672. doi: 10.1038/296671a0. [DOI] [PubMed] [Google Scholar]
  20. Stone L. B., Takemoto K. K. Transformation of mouse macrophages by simian virus 40. J Virol. 1970 Nov;6(5):621–627. doi: 10.1128/jvi.6.5.621-627.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Takayama H. Transformation of mouse peritoneal macrophages and bone marrow cells by simian virus 40. Microbiol Immunol. 1980;24(2):155–167. doi: 10.1111/j.1348-0421.1980.tb00573.x. [DOI] [PubMed] [Google Scholar]
  22. Tanigawa T., Suzuki T., Takayama H., Takagi A. Changes in prostaglandin levels in cultures of SV40 -transformed macrophage cell lines in relation to their phenotypic expression. Microbiol Immunol. 1982;26(1):59–66. doi: 10.1111/j.1348-0421.1982.tb00153.x. [DOI] [PubMed] [Google Scholar]
  23. Treves A. J., Fuks Z., Voss R., Tal T., Barak V., Konijn A. M., Kaplan R., Laskov R. Establishment of cell lines from somatic cell hybrids between human monocytes and mouse myeloma cells. J Immunol. 1984 Feb;132(2):690–694. [PubMed] [Google Scholar]
  24. Uchida T., Ju S., Fay A., Liu Y., Dorf M. E. Functional analysis of macrophage hybridomas. I. Production and initial characterization. J Immunol. 1985 Feb;134(2):772–778. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES