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. 1990 Nov;71(3):411–416.

Murine epidermal antigen-presenting cells in primary and secondary T-cell proliferative responses to a soluble protein antigen in vitro.

N A Williams 1, T J Hill 1, D C Hooper 1
PMCID: PMC1384441  PMID: 2148541

Abstract

The capacity of epidermal cells (EC) to present antigen to primed and non-immune T cells was investigated using a culture system that supports antigen-specific primary and secondary proliferative responses. Although both naive and bovine serum albumin (BSA)-immune T cells reacted against BSA in the presence of either splenic or epidermal antigen-presenting cells (APC), important differences were noted in the kinetics and the magnitudes of the various responses. Most conspicuous was the relatively poor primary response supported by EC which evidently elicited very few BSA-immune T-helper cells. Despite this, the primed antigen-specific T cells recovered were phenotypically similar to those resulting from the stronger primary responses induced by spleen cells. In contrast to this disparity in the ability to prime, EC and spleen cells stimulated secondary reactions of comparable magnitude. We therefore consider that, in comparison with splenic APC, EC may require some additional stimulus to acquire the capacity to prime.

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Selected References

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  1. Austyn J. M., Steinman R. M., Weinstein D. E., Granelli-Piperno A., Palladino M. A. Dendritic cells initiate a two-stage mechanism for T lymphocyte proliferation. J Exp Med. 1983 Apr 1;157(4):1101–1115. doi: 10.1084/jem.157.4.1101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braathen L. R., Berle E., Mobech-Hanssen U., Thorsby E. Studies on human epidermal Langerhans' cells: II. Activation of human T lymphocytes to herpes simplex virus. Acta Derm Venereol. 1980;60(5):381–387. [PubMed] [Google Scholar]
  3. Braathen L. R. Studies on human epidermal Langerhans cells III. Induction of T lymphocyte response to nickel sulphate in sensitized individuals. Br J Dermatol. 1980 Nov;103(5):517–526. doi: 10.1111/j.1365-2133.1980.tb01666.x. [DOI] [PubMed] [Google Scholar]
  4. Haynes B. F., Telen M. J., Hale L. P., Denning S. M. CD44--a molecule involved in leukocyte adherence and T-cell activation. Immunol Today. 1989 Dec;10(12):423–428. doi: 10.1016/0167-5699(89)90040-6. [DOI] [PubMed] [Google Scholar]
  5. Heufler C., Koch F., Schuler G. Granulocyte/macrophage colony-stimulating factor and interleukin 1 mediate the maturation of murine epidermal Langerhans cells into potent immunostimulatory dendritic cells. J Exp Med. 1988 Feb 1;167(2):700–705. doi: 10.1084/jem.167.2.700. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Inaba K., Schuler G., Witmer M. D., Valinksy J., Atassi B., Steinman R. M. Immunologic properties of purified epidermal Langerhans cells. Distinct requirements for stimulation of unprimed and sensitized T lymphocytes. J Exp Med. 1986 Aug 1;164(2):605–613. doi: 10.1084/jem.164.2.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Klinkert W. E., LaBadie J. H., Bowers W. E. Accessory and stimulating properties of dendritic cells and macrophages isolated from various rat tissues. J Exp Med. 1982 Jul 1;156(1):1–19. doi: 10.1084/jem.156.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lassila O., Vainio O., Matzinger P. Can B cells turn on virgin T cells? Nature. 1988 Jul 21;334(6179):253–255. doi: 10.1038/334253a0. [DOI] [PubMed] [Google Scholar]
  9. Merkenschlager M., Terry L., Edwards R., Beverley P. C. Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. Eur J Immunol. 1988 Nov;18(11):1653–1661. doi: 10.1002/eji.1830181102. [DOI] [PubMed] [Google Scholar]
  10. Powrie F., Mason D. The MRC OX-22- CD4+ T cells that help B cells in secondary immune responses derive from naive precursors with the MRC OX-22+ CD4+ phenotype. J Exp Med. 1989 Mar 1;169(3):653–662. doi: 10.1084/jem.169.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Romani N., Koide S., Crowley M., Witmer-Pack M., Livingstone A. M., Fathman C. G., Inaba K., Steinman R. M. Presentation of exogenous protein antigens by dendritic cells to T cell clones. Intact protein is presented best by immature, epidermal Langerhans cells. J Exp Med. 1989 Mar 1;169(3):1169–1178. doi: 10.1084/jem.169.3.1169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sell S. Development of restrictions in the expression of immunoglobulin specificities by lymphoid cells. Transplant Rev. 1970;5:19–44. doi: 10.1111/j.1600-065x.1970.tb00355.x. [DOI] [PubMed] [Google Scholar]
  13. Silberberg-Sinakin I., Thorbecke G. J., Baer R. L., Rosenthal S. A., Berezowsky V. Antigen-bearing langerhans cells in skin, dermal lymphatics and in lymph nodes. Cell Immunol. 1976 Aug;25(2):137–151. doi: 10.1016/0008-8749(76)90105-2. [DOI] [PubMed] [Google Scholar]
  14. Stingl G., Gazze-Stingl L. A., Aberer W., Wolff K. Antigen presentation by murine epidermal langerhans cells and its alteration by ultraviolet B light. J Immunol. 1981 Oct;127(4):1707–1713. [PubMed] [Google Scholar]
  15. Unanue E. R., Allen P. M. Biochemistry and biology of antigen presentation by macrophages. Cell Immunol. 1986 Apr 15;99(1):3–6. doi: 10.1016/0008-8749(86)90208-x. [DOI] [PubMed] [Google Scholar]
  16. Wallace D. L., Beverley P. C. Phenotypic changes associated with activation of CD45RA+ and CD45RO+ T cells. Immunology. 1990 Mar;69(3):460–467. [PMC free article] [PubMed] [Google Scholar]
  17. Yasumoto S., Okabe N., Mori R. Role of epidermal Langerhans cells in resistance to herpes simplex virus infection. Arch Virol. 1986;90(3-4):261–271. doi: 10.1007/BF01317375. [DOI] [PubMed] [Google Scholar]

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