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. 1991 Jun;73(2):212–216.

Down-regulation of receptor antigen in leukotriene B4-induced chemotactic deactivation of human polymorphonuclear leucocytes.

J M Boggs 1, C H Koo 1, E J Goetzl 1
PMCID: PMC1384467  PMID: 1649125

Abstract

Pretreatment of suspensions of human polymorphonuclear leucocytes (PMNL) with leukotriene B4 (LTB4) induces chemotactic deactivation, characterized by diminished expression of high-affinity LTB4 receptors and selectively decreased chemotactic responsiveness of the PMNL to LTB4. Rabbit anti-idiotypic antibodies (a-Id) to mouse monoclonal anti-LTB4, which bind to 60,000-80,000 molecular weight (MW) membrane protein of PMNL receptors for LTB4 in Western blots and block binding of [3H]LTB4 to high-affinity receptors of PMNL, detected a reduction in LTB4 receptor antigen during chemotactic deactivation. Loss of high-affinity receptors for LTB4 from the surface of PMNL deactivated by incubation with 10 nM LTB4 was significant after 1 min and after 20 min reached a mean maximum of 82% and 61%, respectively, as assessed by binding of [3H]LTB4 and a-Id. Inhibitors of PMNL proteases did not prevent the deactivation-induced decreases in surface receptors for LTB4. Disruption of deactivated PMNL and solubilization of membrane proteins failed to expose intracellular LTB4 receptors. Incubation of membranes isolated from PMNL with 100 nM LTB4 resulted in a loss of LTB4 receptors similar to that observed in intact PMNL. Changes in LTB4 receptor protein structure or membrane localization, rather than endocytosis or proteolysis, thus appear to explain the rapidly decreased expression of LTB4 receptors, which results from stimulus-specific deactivation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  3. Gifford L. A., Chernov-Rogan T., Harvey J. P., Koo C. H., Goldman D. W., Goetzl E. J. Recognition of human polymorphonuclear leukocyte receptors for leukotriene B4 by rabbit anti-idiotypic antibodies to a mouse monoclonal antileukotriene B4. J Immunol. 1987 Feb 15;138(4):1184–1189. [PubMed] [Google Scholar]
  4. Goetzl E. J., Pickett W. C. Novel structural determinants of the human neutrophil chemotactic activity of leukotriene B. J Exp Med. 1981 Feb 1;153(2):482–487. doi: 10.1084/jem.153.2.482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldman D. W., Goetzl E. J. Heterogeneity of human polymorphonuclear leukocyte receptors for leukotriene B4. Identification of a subset of high affinity receptors that transduce the chemotactic response. J Exp Med. 1984 Apr 1;159(4):1027–1041. doi: 10.1084/jem.159.4.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldman D. W., Goetzl E. J. Specific binding of leukotriene B4 to receptors on human polymorphonuclear leukocytes. J Immunol. 1982 Oct;129(4):1600–1604. [PubMed] [Google Scholar]
  7. Jesaitis A. J., Bokoch G. M., Tolley J. O., Allen R. A. Lateral segregation of neutrophil chemotactic receptors into actin- and fodrin-rich plasma membrane microdomains depleted in guanyl nucleotide regulatory proteins. J Cell Biol. 1988 Sep;107(3):921–928. doi: 10.1083/jcb.107.3.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jesaitis A. J., Naemura J. R., Painter R. G., Sklar L. A., Cochrane C. G. Intracellular localization of N-formyl chemotactic receptor and Mg2+ dependent ATPase in human granulocytes. Biochim Biophys Acta. 1982 Dec 17;719(3):556–568. doi: 10.1016/0304-4165(82)90246-x. [DOI] [PubMed] [Google Scholar]
  9. Jesaitis A. J., Tolley J. O., Bokoch G. M., Allen R. A. Regulation of chemoattractant receptor interaction with transducing proteins by organizational control in the plasma membrane of human neutrophils. J Cell Biol. 1989 Dec;109(6 Pt 1):2783–2790. doi: 10.1083/jcb.109.6.2783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lee J. Y., Chernov T., Goetzl E. J. Characteristics of the epitope of leukotriene B4 recognized by a highly specific mouse monoclonal antibody. Biochem Biophys Res Commun. 1984 Sep 28;123(3):944–950. doi: 10.1016/s0006-291x(84)80225-9. [DOI] [PubMed] [Google Scholar]
  11. McLeish K. R., Gierschik P., Jakobs K. H. Desensitization uncouples the formyl peptide receptor-guanine nucleotide-binding protein interaction in HL60 cells. Mol Pharmacol. 1989 Sep;36(3):384–390. [PubMed] [Google Scholar]
  12. Palmblad J., Gyllenhammar H., Lindgren J. A., Malmsten C. L. Effects of leukotrienes and f-Met-Leu-Phe on oxidative metabolism of neutrophils and eosinophils. J Immunol. 1984 Jun;132(6):3041–3045. [PubMed] [Google Scholar]
  13. Shalit M., Valone F. H., Atkins P. C., Ratnoff W. D., Goetzl E. J., Zweiman B. Late appearance of phospholipid platelet-activating factor and leukotriene B4 in human skin after repeated antigen challenge. J Allergy Clin Immunol. 1989 Mar;83(3):691–696. doi: 10.1016/0091-6749(89)90084-5. [DOI] [PubMed] [Google Scholar]
  14. Sherman J. W., Goetzl E. J., Koo C. H. Selective modulation by guanine nucleotides of the high affinity subset of plasma membrane receptors for leukotriene B4 on human polymorphonuclear leukocytes. J Immunol. 1988 Jun 1;140(11):3900–3904. [PubMed] [Google Scholar]
  15. Särndahl E., Lindroth M., Bengtsson T., Fällman M., Gustavsson J., Stendahl O., Andersson T. Association of ligand-receptor complexes with actin filaments in human neutrophils: a possible regulatory role for a G-protein. J Cell Biol. 1989 Dec;109(6 Pt 1):2791–2799. doi: 10.1083/jcb.109.6.2791. [DOI] [PMC free article] [PubMed] [Google Scholar]

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