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. 1991 Feb;72(2):194–198.

Release of a suppressor cell-inducing factor by monocytes from patients with pulmonary tuberculosis.

H Fujiwara 1, K Ohnishi 1, S Kishimoto 1, J J Ellner 1, I Tsuyuguchi 1
PMCID: PMC1384483  PMID: 1826668

Abstract

We observed that blood monocytes from tuberculous patients secreted a factor which induced suppressor cells. Monocyte-enriched adherent cells were prepared from peripheral blood mononuclear cells (PBMC) of 14 patients with active pulmonary tuberculosis or nine healthy tuberculin skin reactors and were cultured without any stimulus for 5 days. For induction of suppressor cells, PBMC from a healthy tuberculin reactor were cultured with the monocyte culture supernatants (MN SUP). After 2 days, those cells were washed and irradiated, then tested for their ability to suppress blastogenesis in autologous PBMC stimulated with purified protein derivative (PPD). Mean activity of suppressor cell induction in MN SUP of patients with pulmonary tuberculosis was significantly greater than that of healthy controls. In contrast, PPD-induced blastogenic responses in PBMC from tuberculous patients were lower than in controls. When we divided those tuberculous patients into high and low secretor groups on the basis of the magnitude of suppressor cell induction by MN SUP, PBMC from the high secretors had lower direct blastogenic responses to PPD than those of the low secretors. The blastogenic responses of PBMC from the high secretors, but not the low secretors, were significantly lower than those of healthy subjects. MN SUP which contained high suppressor cell-inducing activity had no effect on PPD-induced blastogenesis of PBMC from a healthy donor. These data suggest that monocytes from some tuberculous patients indirectly depress lymphocyte blastogenesis through a suppressor cell-inducing factor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  2. Ellner J. J., Spagnuolo P. J. Suppression of antigen and mitogen induced human T lymphocyte DNA synthesis by bacterial lipopolysaccharide: mediation by monocyte activation and production of prostaglandins. J Immunol. 1979 Dec;123(6):2689–2695. [PubMed] [Google Scholar]
  3. Ellner J. J. Suppressor adherent cells in human tuberculosis. J Immunol. 1978 Dec;121(6):2573–2579. [PubMed] [Google Scholar]
  4. Fujiwara H., Kleinhenz M. E., Wallis R. S., Ellner J. J. Increased interleukin-1 production and monocyte suppressor cell activity associated with human tuberculosis. Am Rev Respir Dis. 1986 Jan;133(1):73–77. doi: 10.1164/arrd.1986.133.1.73. [DOI] [PubMed] [Google Scholar]
  5. Fujiwara H., Okuda Y., Fukukawa T., Tsuyuguchi I. In vitro tuberculin reactivity of lymphocytes from patients with tuberculous pleurisy. Infect Immun. 1982 Feb;35(2):402–409. doi: 10.1128/iai.35.2.402-409.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gordon D., Bray M. A., Morley J. Control of lymphokine secretion by prostaglandins. Nature. 1976 Jul 29;262(5567):401–402. doi: 10.1038/262401a0. [DOI] [PubMed] [Google Scholar]
  7. Metzger Z., Hoffeld J. T., Oppenheim J. J. Macrophage-mediated suppression. I. Evidence for participation of both hdyrogen peroxide and prostaglandins in suppression of murine lymphocyte proliferation. J Immunol. 1980 Feb;124(2):983–988. [PubMed] [Google Scholar]
  8. Salgame P. R., Mahadevan P. R., Antia N. H. Mechanism of immunosuppression in leprosy: presence of suppressor factor(s) from macrophages of lepromatous patients. Infect Immun. 1983 Jun;40(3):1119–1126. doi: 10.1128/iai.40.3.1119-1126.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Stadecker M. J., Calderon J., Karnovsky M. L., Unanue E. R. Synthesis and release of thymidine by macrophages. J Immunol. 1977 Nov;119(5):1738–1743. [PubMed] [Google Scholar]
  10. Stobo J. D. Immunosuppression in man: suppression by macrophages can be mediated by interactions with regulatory T cells. J Immunol. 1977 Sep;119(3):918–924. [PubMed] [Google Scholar]
  11. Toossi Z., Sedor J. R., Lapurga J. P., Ondash R. J., Ellner J. J. Expression of functional interleukin 2 receptors by peripheral blood monocytes from patients with active pulmonary tuberculosis. J Clin Invest. 1990 Jun;85(6):1777–1784. doi: 10.1172/JCI114635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tweardy D. J., Schacter B. Z., Ellner J. J. Association of altered dynamics of monocyte surface expression of human leukocyte antigen DR with immunosuppression in tuberculosis. J Infect Dis. 1984 Jan;149(1):31–37. doi: 10.1093/infdis/149.1.31. [DOI] [PubMed] [Google Scholar]
  13. Valone S. E., Rich E. A., Wallis R. S., Ellner J. J. Expression of tumor necrosis factor in vitro by human mononuclear phagocytes stimulated with whole Mycobacterium bovis BCG and mycobacterial antigens. Infect Immun. 1988 Dec;56(12):3313–3315. doi: 10.1128/iai.56.12.3313-3315.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wadee A. A., Mendelsohn D., Rabson A. R. Characterization of a suppressor cell-activating factor (SCAF) released by adherent cells treated with M. tuberculosis. J Immunol. 1983 May;130(5):2266–2270. [PubMed] [Google Scholar]
  15. Wallis R. S., Fujiwara H., Ellner J. J. Direct stimulation of monocyte release of interleukin 1 by mycobacterial protein antigens. J Immunol. 1986 Jan;136(1):193–196. [PubMed] [Google Scholar]

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