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. 1991 May;73(1):19–23.

In vivo adjuvant effect of interleukins 5 and 6 on rat tear IgA antibody responses.

A G Pockley 1, P C Montgomery 1
PMCID: PMC1384512  PMID: 2045126

Abstract

Interleukins 5 and 6 have previously been shown to exhibit IgA-enhancing activity in vitro. This study investigated the effect of IL-5 and IL-6 on antigen-specific tear IgA antibody responses in vivo. Rats were given primary and secondary immunizations by the ocular-topical (OT) route with either antigen (DNP-Pn) alone or antigen plus IL-5 and IL-6 (IL-5/6). Antigen was administered for the first 3 days of each stimulation cycle and lymphokines (50 units of each lymphokine/eye/day) or control media for the first 7 days of the primary and secondary immunizations. Co-administration of antigen with IL-5/6 markedly enhanced (approximately three-fold) the secondary tear IgA anti-DNP antibody response in the lymphokine-treated group. Enhanced tear IgA antibody responses were maintained in the lymphokine-treated group following a tertiary immunization in the absence of IL-5/6. There was no apparent effect on the total tear IgA levels in either group of animals. Ocular topical immunization also elicited secondary and tertiary serum IgG antibody responses which were not affected by the administration of IL-5/6. Neither secondary nor tertiary serum IgM antibody responses were detected in either group of animals. These data indicate that IL-5 and IL-6 are able to augment tear IgA antibody responses in vivo. That the enhancement was maintained following a subsequent antigenic challenge in the absence of the lymphokines suggests a potential therapeutic application for lymphokines in augmenting IgA responses at mucosal surfaces.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson A. O., Rubin D. H. Effect of Avridine on enteric antigen uptake and mucosal immunity to reovirus (1/Lang). Adv Exp Med Biol. 1985;186:579–590. doi: 10.1007/978-1-4613-2463-8_71. [DOI] [PubMed] [Google Scholar]
  2. Beagley K. W., Eldridge J. H., Kiyono H., Everson M. P., Koopman W. J., Honjo T., McGhee J. R. Recombinant murine IL-5 induces high rate IgA synthesis in cycling IgA-positive Peyer's patch B cells. J Immunol. 1988 Sep 15;141(6):2035–2042. [PubMed] [Google Scholar]
  3. Beagley K. W., Eldridge J. H., Lee F., Kiyono H., Everson M. P., Koopman W. J., Hirano T., Kishimoto T., McGhee J. R. Interleukins and IgA synthesis. Human and murine interleukin 6 induce high rate IgA secretion in IgA-committed B cells. J Exp Med. 1989 Jun 1;169(6):2133–2148. doi: 10.1084/jem.169.6.2133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burns C. A., Ebersole J. L., Allansmith M. R. Immunoglobulin A antibody levels in human tears, saliva, and serum. Infect Immun. 1982 Jun;36(3):1019–1022. doi: 10.1128/iai.36.3.1019-1022.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coffman R. L., Shrader B., Carty J., Mosmann T. R., Bond M. W. A mouse T cell product that preferentially enhances IgA production. I. Biologic characterization. J Immunol. 1987 Dec 1;139(11):3685–3690. [PubMed] [Google Scholar]
  6. Dutton R. W., Wetzel G. D., Swain S. L. Partial purification and characterization of a BCGFII from EL4 culture supernatants. J Immunol. 1984 May;132(5):2451–2456. [PubMed] [Google Scholar]
  7. EISEN H. N. PREPARATION OF PURIFIED ANTI-2,4-DINITROPHENYL ANTIBODIES. Methods Med Res. 1964;10:94–102. [PubMed] [Google Scholar]
  8. Elson C. O., Ealding W. Cholera toxin feeding did not induce oral tolerance in mice and abrogated oral tolerance to an unrelated protein antigen. J Immunol. 1984 Dec;133(6):2892–2897. [PubMed] [Google Scholar]
  9. Gudmundsson O. G., Sullivan D. A., Bloch K. J., Allansmith M. R. The ocular secretory immune system of the rat. Exp Eye Res. 1985 Feb;40(2):231–238. doi: 10.1016/0014-4835(85)90008-9. [DOI] [PubMed] [Google Scholar]
  10. Harriman G. R., Kunimoto D. Y., Elliott J. F., Paetkau V., Strober W. The role of IL-5 in IgA B cell differentiation. J Immunol. 1988 May 1;140(9):3033–3039. [PubMed] [Google Scholar]
  11. Kawamura H., Rosenberg S. A., Berzofsky J. A. Immunization with antigen and interleukin 2 in vivo overcomes Ir gene low responsiveness. J Exp Med. 1985 Jul 1;162(1):381–386. doi: 10.1084/jem.162.1.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kunimoto D. Y., Nordan R. P., Strober W. IL-6 is a potent cofactor of IL-1 in IgM synthesis and of IL-5 in IgA synthesis. J Immunol. 1989 Oct 1;143(7):2230–2235. [PubMed] [Google Scholar]
  13. McDermott M. R., Bienenstock J. Evidence for a common mucosal immunologic system. I. Migration of B immunoblasts into intestinal, respiratory, and genital tissues. J Immunol. 1979 May;122(5):1892–1898. [PubMed] [Google Scholar]
  14. Miyajima A., Miyatake S., Schreurs J., De Vries J., Arai N., Yokota T., Arai K. Coordinate regulation of immune and inflammatory responses by T cell-derived lymphokines. FASEB J. 1988 Jun;2(9):2462–2473. doi: 10.1096/fasebj.2.9.2836253. [DOI] [PubMed] [Google Scholar]
  15. Montgomery P. C., Ayyildiz A., Lemaitre-Coelho I. M., Vaerman J. P., Rockey J. H. Induction and expression of antibodies in secretions: the ocular immune system. Ann N Y Acad Sci. 1983 Jun 30;409:428–440. doi: 10.1111/j.1749-6632.1983.tb26887.x. [DOI] [PubMed] [Google Scholar]
  16. Montgomery P. C., Pincus J. H. Molecular restriction of anti-DNP antibodies induced by dinitrophenylated type 3 pneumococcus. J Immunol. 1973 Jul;111(1):42–51. [PubMed] [Google Scholar]
  17. Orlans E., Peppard J., Reynolds J., Hall J. Rapid active transport of immunoglobulin A from blood to bile. J Exp Med. 1978 Feb 1;147(2):588–592. doi: 10.1084/jem.147.2.588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Peppard J. V., Mann R. V., Montgomery P. C. Antibody production in rats following ocular-topical or gastrointestinal immunization: kinetics of local and systemic antibody production. Curr Eye Res. 1988 May;7(5):471–481. doi: 10.3109/02713688809031800. [DOI] [PubMed] [Google Scholar]
  19. Peppard J. V., Montgomery P. C. Studies on the origin and composition of IgA in rat tears. Immunology. 1987 Oct;62(2):193–198. [PMC free article] [PubMed] [Google Scholar]
  20. Pierce N. F., Sacci J. B., Jr Enhanced mucosal priming by cholera toxin and procholeragenoid with a lipoidal amine adjuvant (avridine) delivered in liposomes. Infect Immun. 1984 May;44(2):469–473. doi: 10.1128/iai.44.2.469-473.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Roux M. E., McWilliams M., Phillips-Quagliata J. M., Weisz-Carrington P., Lamm M. E. Origin of IgA-secreting plasma cells in the mammary gland. J Exp Med. 1977 Nov 1;146(5):1311–1322. doi: 10.1084/jem.146.5.1311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sullivan D. A., Allansmith M. R. Source of IgA in tears of rats. Immunology. 1984 Dec;53(4):791–799. [PMC free article] [PubMed] [Google Scholar]
  23. Taubman M. A., Ebersole J. L., Smith D. J., Stack W. Adjuvants for secretory immune responses. Ann N Y Acad Sci. 1983 Jun 30;409:637–649. doi: 10.1111/j.1749-6632.1983.tb26905.x. [DOI] [PubMed] [Google Scholar]
  24. Tonkonogy S. L., McKenzie D. T., Swain S. L. Regulation of isotype production by IL-4 and IL-5. Effects of lymphokines on Ig production depend on the state of activation of the responding B cells. J Immunol. 1989 Jun 15;142(12):4351–4360. [PubMed] [Google Scholar]
  25. Weinberg A., Merigan T. C. Recombinant interleukin 2 as an adjuvant for vaccine-induced protection. Immunization of guinea pigs with herpes simplex virus subunit vaccines. J Immunol. 1988 Jan 1;140(1):294–299. [PubMed] [Google Scholar]
  26. Weyand C. M., Goronzy J., Dallman M. J., Fathman C. G. Administration of recombinant interleukin 2 in vivo induces a polyclonal IgM response. J Exp Med. 1986 Jun 1;163(6):1607–1612. doi: 10.1084/jem.163.6.1607. [DOI] [PMC free article] [PubMed] [Google Scholar]

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