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. 1991 Jul;73(3):298–303.

Biochemical and developmental characterization of the murine cluster of differentiation 1 antigen.

D D Mosser 1, J Duchaine 1, L H Martin 1
PMCID: PMC1384546  PMID: 1715316

Abstract

The cluster of differentiation-1 (CD1) antigens are major histocompatibility complex (MHC) class I-like glycoproteins belonging to the immunoglobulin supergene family. Initially described in humans, more recently putative CD1 encoding genes have been identified in several other species, including the mouse where it has been clearly demonstrated that CD1 mRNA is expressed. However, in the mouse both its unusually wide tissue distribution and the prevalence of incompletely spliced RNA have raised the possibility that the mRNA did not encode a functional protein. We have utilized a rabbit polyclonal antiserum raised against an Escherichia coli-expressed recombinant murine CD1 fusion protein to characterize the murine CD1 protein. Here we demonstrate that the antiserum binds specifically to a set of glycoproteins (49,000-55,000 MW) which contain a common core protein with both a size (36,000 MW) and tissue distribution in accordance with those predicted. During thymic ontogeny, this protein is highly expressed by Day 14 of embryonic development and persists into adulthood, while its pattern of expression in other organs changes significantly during development. Thus, the mouse provides an amenable model system for the study of CD1 function.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albertson D. G., Fishpool R., Sherrington P., Nacheva E., Milstein C. Sensitive and high resolution in situ hybridization to human chromosomes using biotin labelled probes: assignment of the human thymocyte CD1 antigen genes to chromosome 1. EMBO J. 1988 Sep;7(9):2801–2805. doi: 10.1002/j.1460-2075.1988.tb03135.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Amiot M., Dastot H., Degos L., Dausset J., Bernard A., Boumsell L. HLA class I molecules are associated with CD1a heavy chains on normal human thymus cells. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4451–4454. doi: 10.1073/pnas.85.12.4451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bleicher P. A., Balk S. P., Hagen S. J., Blumberg R. S., Flotte T. J., Terhorst C. Expression of murine CD1 on gastrointestinal epithelium. Science. 1990 Nov 2;250(4981):679–682. doi: 10.1126/science.1700477. [DOI] [PubMed] [Google Scholar]
  4. Bradbury A., Belt K. T., Neri T. M., Milstein C., Calabi F. Mouse CD1 is distinct from and co-exists with TL in the same thymus. EMBO J. 1988 Oct;7(10):3081–3086. doi: 10.1002/j.1460-2075.1988.tb03173.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bradbury A., Calabi F., Milstein C. Expression of CD1 in the mouse thymus. Eur J Immunol. 1990 Aug;20(8):1831–1836. doi: 10.1002/eji.1830200830. [DOI] [PubMed] [Google Scholar]
  6. Calabi F., Belt K. T., Yu C. Y., Bradbury A., Mandy W. J., Milstein C. The rabbit CD1 and the evolutionary conservation of the CD1 gene family. Immunogenetics. 1989;30(5):370–377. doi: 10.1007/BF02425277. [DOI] [PubMed] [Google Scholar]
  7. Calabi F., Jarvis J. M., Martin L., Milstein C. Two classes of CD1 genes. Eur J Immunol. 1989 Feb;19(2):285–292. doi: 10.1002/eji.1830190211. [DOI] [PubMed] [Google Scholar]
  8. Calabi F., Milstein C. A novel family of human major histocompatibility complex-related genes not mapping to chromosome 6. Nature. 1986 Oct 9;323(6088):540–543. doi: 10.1038/323540a0. [DOI] [PubMed] [Google Scholar]
  9. Ey P. L., Ashman L. K. The use of alkaline phosphatase-conjugated anti-immunoglobulin with immunoblots for determining the specificity of monoclonal antibodies to protein mixtures. Methods Enzymol. 1986;121:497–509. doi: 10.1016/0076-6879(86)21050-2. [DOI] [PubMed] [Google Scholar]
  10. Knowles R. W., Bodmer W. F. A monoclonal antibody recognizing a human thymus leukemia-like antigen associated with beta 2-microglobulin. Eur J Immunol. 1982 Aug;12(8):676–681. doi: 10.1002/eji.1830120810. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Ledbetter J. A., Tsu T. T., Clark E. A. Covalent association between human thymus leukemia-like antigens and CD8(Tp32) molecules. J Immunol. 1985 Jun;134(6):4250–4254. [PubMed] [Google Scholar]
  13. Martin L. H., Calabi F., Lefebvre F. A., Bilsland C. A., Milstein C. Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9189–9193. doi: 10.1073/pnas.84.24.9189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Martin L. H., Calabi F., Milstein C. Isolation of CD1 genes: a family of major histocompatibility complex-related differentiation antigens. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9154–9158. doi: 10.1073/pnas.83.23.9154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McMichael A. J., Pilch J. R., Galfré G., Mason D. Y., Fabre J. W., Milstein C. A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur J Immunol. 1979 Mar;9(3):205–210. doi: 10.1002/eji.1830090307. [DOI] [PubMed] [Google Scholar]
  16. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  17. Porcelli S., Brenner M. B., Greenstein J. L., Balk S. P., Terhorst C., Bleicher P. A. Recognition of cluster of differentiation 1 antigens by human CD4-CD8-cytolytic T lymphocytes. Nature. 1989 Oct 5;341(6241):447–450. doi: 10.1038/341447a0. [DOI] [PubMed] [Google Scholar]
  18. Reinherz E. L., Schlossman S. F. The differentiation and function of human T lymphocytes. Cell. 1980 Apr;19(4):821–827. doi: 10.1016/0092-8674(80)90072-0. [DOI] [PubMed] [Google Scholar]
  19. Snow P. M., Van de Rijn M., Terhorst C. Association between the human thymic differentiation antigens T6 and T8. Eur J Immunol. 1985 May;15(5):529–532. doi: 10.1002/eji.1830150520. [DOI] [PubMed] [Google Scholar]
  20. Theodorou I. D., Boumsell L., Calvo C. F., Gouy H., Beral H. M., Debre P. CD1 stimulation of human T cell lines induces a rapid increase in the intracellular free Ca2+ concentration and the production of IL-2. J Immunol. 1990 Apr 1;144(7):2518–2523. [PubMed] [Google Scholar]
  21. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wang C. R., Chen G. H., Mandy W. J. Identification of a rabbit class I-like thymocyte-specific antigen. J Immunol. 1987 May 15;138(10):3352–3359. [PubMed] [Google Scholar]

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