Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1991 Aug;73(4):472–477.

IL-8 causes in vivo neutrophil migration by a cell-dependent mechanism.

R A Ribeiro 1, C A Flores 1, F Q Cunha 1, S H Ferreira 1
PMCID: PMC1384579  PMID: 1916898

Abstract

A dose-dependent neutrophil migration was observed following the injection of recombinant interleukin-8 (rIL-8) into rat peritoneal cavities. This finding contrasts with the inability of rIL-8 to induce neutrophil emigration into subcutaneous air-pouches. Pre-treatment of the animals with dexamethasone (0.5 mg/kg, s.c.) or depletion of the peritoneal resident cell population abolished the neutrophil migration induced by rIL-8 and by recombinant interleukin-1 beta (rIL-1 beta). Different from that which occurs with rIL-1 beta, neutrophil migration induced by rIL-8 was not enhanced by an increase in the peritoneal macrophage population. Transference of homologous total resident peritoneal cells to the air-pouch rendered this cavity responsive to the chemotactic effect of rIL-8 and potentiated the neutrophil migration induced by rIL-1. Our results show that both rIL-8 and rIL-1 beta are able to induce in vivo neutrophil migration by an indirect mechanism, dependent on resident cells. Neither macrophages nor lymphocytes seem to be involved in the rIL-8 chemotactic effect. However, peritoneal resident mast cells may be implicated in this mechanism. These cells, when stimulated in vitro by rIL-8, released a factor that when injected into peritoneal and air-pouch cavities induced neutrophil migration.

Full text

PDF
472

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beutler B., Krochin N., Milsark I. W., Luedke C., Cerami A. Control of cachectin (tumor necrosis factor) synthesis: mechanisms of endotoxin resistance. Science. 1986 May 23;232(4753):977–980. doi: 10.1126/science.3754653. [DOI] [PubMed] [Google Scholar]
  2. Boyle M. D., Lawman M. J., Gee A. P., Young M. Measurement of leukocyte chemotaxis in vivo. Methods Enzymol. 1988;162:101–114. doi: 10.1016/0076-6879(88)62068-4. [DOI] [PubMed] [Google Scholar]
  3. Colditz I., Zwahlen R., Dewald B., Baggiolini M. In vivo inflammatory activity of neutrophil-activating factor, a novel chemotactic peptide derived from human monocytes. Am J Pathol. 1989 Apr;134(4):755–760. [PMC free article] [PubMed] [Google Scholar]
  4. Cunha F. Q., Ferreira S. H. The release of a neutrophil chemotactic factor from peritoneal macrophages by endotoxin: inhibition by glucocorticoids. Eur J Pharmacol. 1986 Sep 23;129(1-2):65–76. doi: 10.1016/0014-2999(86)90337-7. [DOI] [PubMed] [Google Scholar]
  5. Edwards J. C., Sedgwick A. D., Willoughby D. A. The formation of a structure with the features of synovial lining by subcutaneous injection of air: an in vivo tissue culture system. J Pathol. 1981 Jun;134(2):147–156. doi: 10.1002/path.1711340205. [DOI] [PubMed] [Google Scholar]
  6. Enerbäck L., Svensson I. Isolation of rat peritoneal mast cells by centrifugation on density gradients of Percoll. J Immunol Methods. 1980;39(1-2):135–145. doi: 10.1016/0022-1759(80)90302-6. [DOI] [PubMed] [Google Scholar]
  7. Faccioli L. H., Souza G. E., Cunha F. Q., Poole S., Ferreira S. H. Recombinant interleukin-1 and tumor necrosis factor induce neutrophil migration "in vivo" by indirect mechanisms. Agents Actions. 1990 Jun;30(3-4):344–349. doi: 10.1007/BF01966298. [DOI] [PubMed] [Google Scholar]
  8. Fincham N. J., Camp R. D., Gearing A. J., Bird C. R., Cunningham F. M. Neutrophil chemoattractant and IL-1-like activity in samples from psoriatic skin lesions. Further characterization. J Immunol. 1988 Jun 15;140(12):4294–4299. [PubMed] [Google Scholar]
  9. Gimbrone M. A., Jr, Obin M. S., Brock A. F., Luis E. A., Hass P. E., Hébert C. A., Yip Y. K., Leung D. W., Lowe D. G., Kohr W. J. Endothelial interleukin-8: a novel inhibitor of leukocyte-endothelial interactions. Science. 1989 Dec 22;246(4937):1601–1603. doi: 10.1126/science.2688092. [DOI] [PubMed] [Google Scholar]
  10. Gregory H., Young J., Schröder J. M., Mrowietz U., Christophers E. Structure determination of a human lymphocyte derived neutrophil activating peptide (LYNAP). Biochem Biophys Res Commun. 1988 Mar 15;151(2):883–890. doi: 10.1016/s0006-291x(88)80364-4. [DOI] [PubMed] [Google Scholar]
  11. Harmsen A. G., Havell E. A. Roles of tumor necrosis factor and macrophages in lipopolysaccharide-induced accumulation of neutrophils in cutaneous air pouches. Infect Immun. 1990 Feb;58(2):297–302. doi: 10.1128/iai.58.2.297-302.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Katori M., Oda T., Nagai K. A site of action of dexamethasone on leukocyte extravasation in microcirculation. Agents Actions. 1990 Jan;29(1-2):24–26. doi: 10.1007/BF01964709. [DOI] [PubMed] [Google Scholar]
  13. Kowanko I. C., Gordon T. P., Rozenbilds M. A., Brooks P. M., Roberts-Thomson P. J. The subcutaneous air pouch model of synovium and the inflammatory response to heat aggregated gammaglobulin. Agents Actions. 1986 Jun;18(3-4):421–428. doi: 10.1007/BF01965007. [DOI] [PubMed] [Google Scholar]
  14. Leme J. G., Bechara G. H., Dos Santos R. R. A proinflammatory factor in lymphocytes. Its role in the development of acute, non-immunological inflammatory reactions. Br J Exp Pathol. 1976 Aug;57(4):377–386. [PMC free article] [PubMed] [Google Scholar]
  15. Lindley I., Aschauer H., Seifert J. M., Lam C., Brunowsky W., Kownatzki E., Thelen M., Peveri P., Dewald B., von Tscharner V. Synthesis and expression in Escherichia coli of the gene encoding monocyte-derived neutrophil-activating factor: biological equivalence between natural and recombinant neutrophil-activating factor. Proc Natl Acad Sci U S A. 1988 Dec;85(23):9199–9203. doi: 10.1073/pnas.85.23.9199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Matsushima K., Morishita K., Yoshimura T., Lavu S., Kobayashi Y., Lew W., Appella E., Kung H. F., Leonard E. J., Oppenheim J. J. Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor. J Exp Med. 1988 Jun 1;167(6):1883–1893. doi: 10.1084/jem.167.6.1883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rampart M., Van Damme J., Zonnekeyn L., Herman A. G. Granulocyte chemotactic protein/interleukin-8 induces plasma leakage and neutrophil accumulation in rabbit skin. Am J Pathol. 1989 Jul;135(1):21–25. [PMC free article] [PubMed] [Google Scholar]
  19. Rampart M., Williams T. J. Evidence that neutrophil accumulation induced by interleukin-1 requires both local protein biosynthesis and neutrophil CD18 antigen expression in vivo. Br J Pharmacol. 1988 Aug;94(4):1143–1148. doi: 10.1111/j.1476-5381.1988.tb11632.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ribeiro R. A., Cunha F. Q., Ferreira S. H. Recombinant gamma interferon causes neutrophil migration mediated by the release of a macrophage neutrophil chemotactic factor. Int J Exp Pathol. 1990 Oct;71(5):717–725. [PMC free article] [PubMed] [Google Scholar]
  21. Schröder J. M., Mrowietz U., Morita E., Christophers E. Purification and partial biochemical characterization of a human monocyte-derived, neutrophil-activating peptide that lacks interleukin 1 activity. J Immunol. 1987 Nov 15;139(10):3474–3483. [PubMed] [Google Scholar]
  22. Souza G. E., Cunha F. Q., Mello R., Ferreira S. H. Neutrophil migration induced by inflammatory stimuli is reduced by macrophage depletion. Agents Actions. 1988 Jul;24(3-4):377–380. doi: 10.1007/BF02028296. [DOI] [PubMed] [Google Scholar]
  23. Walz A., Peveri P., Aschauer H., Baggiolini M. Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes. Biochem Biophys Res Commun. 1987 Dec 16;149(2):755–761. doi: 10.1016/0006-291x(87)90432-3. [DOI] [PubMed] [Google Scholar]
  24. White M. V., Yoshimura T., Hook W., Kaliner M. A., Leonard E. J. Neutrophil attractant/activation protein-1 (NAP-1) causes human basophil histamine release. Immunol Lett. 1989 Aug;22(2):151–154. doi: 10.1016/0165-2478(89)90182-x. [DOI] [PubMed] [Google Scholar]
  25. Yoshimura T., Matsushima K., Oppenheim J. J., Leonard E. J. Neutrophil chemotactic factor produced by lipopolysaccharide (LPS)-stimulated human blood mononuclear leukocytes: partial characterization and separation from interleukin 1 (IL 1). J Immunol. 1987 Aug 1;139(3):788–793. [PubMed] [Google Scholar]
  26. Yoshimura T., Matsushima K., Tanaka S., Robinson E. A., Appella E., Oppenheim J. J., Leonard E. J. Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defense cytokines. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9233–9237. doi: 10.1073/pnas.84.24.9233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. de Souza G. E., Ferreira S. H. Blockade by antimacrophage serum of the migration of PMN neutrophils into the inflamed peritoneal cavity. Agents Actions. 1985 Oct;17(1):97–103. doi: 10.1007/BF01966691. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES