Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1991 Aug;73(4):498–504.

Regulation of Forssman antigen expression during maturation of mouse stromal macrophages in haematopoietic foci.

Y Sadahira 1, T Yasuda 1, T Kimoto 1
PMCID: PMC1384583  PMID: 1916901

Abstract

During a trial to develop a monoclonal antibody (mAb) specific to stromal macrophages (M phi) in haematopoietic foci, we have created a mAb, designated F10, that stains the stromal M phi more selectively than any other mAb reported. As F10 was found to react with Forssman glycosphingolipid (GSL) specifically and to give clearer immunostaining than anti-Forssman GSL IgG, we have studied Forssman antigen expression during maturation of the stromal M phi in splenic haematopoietic foci using F10 and a system of allogenic bone marrow transplantation which allows us to know the turnover of the stromal M phi in vivo. C3H/He mice (H-2k) were lethally irradiated and intravenously infused with the bone marrow cells of BALB/c nu-nu mice (H-2d). Splenic frozen-sections and cytocentrifuge preparations of splenic haematopoietic clusters from the recipient mice were stained with F10 and with mAb against major histocompatibility class I antigens. H-2d-type stromal M phi began to appear in the haematopoietic clusters at Week 5 and they gradually replaced H-2k-type stromal M phi. The percentage of Forssman+ stromal M phi gradually decreased and reached a nadir at Week 6, when most stromal M phi were already of the donor type. At Week 8, however, Forssman+ stromal M phi levels returned to normal. The delayed expression of Forssman antigen on the stromal M phi in haematopoietic foci following genotypic conversion suggests that Forssman antigen is regularly expressed on the subpopulation of stromal M phi, which mature well under specific microenvironmental factors in vivo.

Full text

PDF
498

Images in this article

499Figure 1
on p.499
501Figure 3
on p.501
501Figure 4
on p.501
503Figure 6
on p.503

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austyn J. M., Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur J Immunol. 1981 Oct;11(10):805–815. doi: 10.1002/eji.1830111013. [DOI] [PubMed] [Google Scholar]
  2. Crocker P. R., Gordon S. Isolation and characterization of resident stromal macrophages and hematopoietic cell clusters from mouse bone marrow. J Exp Med. 1985 Sep 1;162(3):993–1014. doi: 10.1084/jem.162.3.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crocker P. R., Gordon S. Mouse macrophage hemagglutinin (sheep erythrocyte receptor) with specificity for sialylated glycoconjugates characterized by a monoclonal antibody. J Exp Med. 1989 Apr 1;169(4):1333–1346. doi: 10.1084/jem.169.4.1333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crocker P. R., Gordon S. Properties and distribution of a lectin-like hemagglutinin differentially expressed by murine stromal tissue macrophages. J Exp Med. 1986 Dec 1;164(6):1862–1875. doi: 10.1084/jem.164.6.1862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crocker P. R., Morris L., Gordon S. Novel cell surface adhesion receptors involved in interactions between stromal macrophages and haematopoietic cells. J Cell Sci Suppl. 1988;9:185–206. doi: 10.1242/jcs.1988.supplement_9.10. [DOI] [PubMed] [Google Scholar]
  6. Crocker P. R., Werb Z., Gordon S., Bainton D. F. Ultrastructural localization of a macrophage-restricted sialic acid binding hemagglutinin, SER, in macrophage-hematopoietic cell clusters. Blood. 1990 Sep 15;76(6):1131–1138. [PubMed] [Google Scholar]
  7. Hakomori S. Bifunctional role of glycosphingolipids. Modulators for transmembrane signaling and mediators for cellular interactions. J Biol Chem. 1990 Nov 5;265(31):18713–18716. [PubMed] [Google Scholar]
  8. Hume D. A., Robinson A. P., MacPherson G. G., Gordon S. The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs. J Exp Med. 1983 Nov 1;158(5):1522–1536. doi: 10.1084/jem.158.5.1522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Humphrey J. H., Sundaram V. Origin and turnover of follicular dendritic cells and marginal zone macrophages in the mouse spleen. Adv Exp Med Biol. 1985;186:167–170. doi: 10.1007/978-1-4613-2463-8_21. [DOI] [PubMed] [Google Scholar]
  10. Ikehara S., Good R. A., Nakamura T., Sekita K., Inoue S., Oo M. M., Muso E., Ogawa K., Hamashima Y. Rationale for bone marrow transplantation in the treatment of autoimmune diseases. Proc Natl Acad Sci U S A. 1985 Apr;82(8):2483–2487. doi: 10.1073/pnas.82.8.2483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kasai M., Iwamori M., Nagai Y., Okumura K., Tada T. A glycolipid on the surface of mouse natural killer cells. Eur J Immunol. 1980 Mar;10(3):175–180. doi: 10.1002/eji.1830100304. [DOI] [PubMed] [Google Scholar]
  12. Kraal G., Rep M., Janse M. Macrophages in T and B cell compartments and other tissue macrophages recognized by monoclonal antibody MOMA-2. An immunohistochemical study. Scand J Immunol. 1987 Dec;26(6):653–661. doi: 10.1111/j.1365-3083.1987.tb02301.x. [DOI] [PubMed] [Google Scholar]
  13. LEDUC E. H., TANAKA N. A study of the cellular distribution of Forssman antigen in various species. J Immunol. 1956 Sep;77(3):198–212. [PubMed] [Google Scholar]
  14. Mori M., Sadahira Y., Kawasaki S., Hayashi T., Awai M. Macrophage heterogeneity in bone marrow culture in vitro. J Cell Sci. 1990 Mar;95(Pt 3):481–485. doi: 10.1242/jcs.95.3.481. [DOI] [PubMed] [Google Scholar]
  15. Phillips M. L., Nudelman E., Gaeta F. C., Perez M., Singhal A. K., Hakomori S., Paulson J. C. ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex. Science. 1990 Nov 23;250(4984):1130–1132. doi: 10.1126/science.1701274. [DOI] [PubMed] [Google Scholar]
  16. Sadahira Y., Mori M., Awai M., Watarai S., Yasuda T. Forssman glycosphingolipid as an immunohistochemical marker for mouse stromal macrophages in hematopoietic foci. Blood. 1988 Jul;72(1):42–48. [PubMed] [Google Scholar]
  17. Sadahira Y., Mori M., Kimoto T. Isolation and short-term culture of mouse splenic erythroblastic islands. Cell Struct Funct. 1990 Feb;15(1):59–65. doi: 10.1247/csf.15.59. [DOI] [PubMed] [Google Scholar]
  18. Sadahira Y., Mori M., Kimoto T. Participation of radioresistant Forssman antigen-bearing macrophages in the formation of stromal elements of erythroid spleen colonies. Br J Haematol. 1989 Apr;71(4):469–474. doi: 10.1111/j.1365-2141.1989.tb06304.x. [DOI] [PubMed] [Google Scholar]
  19. Willison K. R., Stern P. L. Expression of a Forssman antigenic specificity in the preimplantation mouse embryo. Cell. 1978 Aug;14(4):785–793. doi: 10.1016/0092-8674(78)90334-3. [DOI] [PubMed] [Google Scholar]
  20. von Kleist R., Schmitt E., Westermann J., Mühlradt P. F. Modulation of Forssman glycosphingolipid expression by murine macrophages: coinduction with class II MHC antigen by the lymphokines IL4 and IL6. Immunobiology. 1990 Jun;180(4-5):405–418. doi: 10.1016/s0171-2985(11)80302-5. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES