Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1991 Oct;74(2):338–341.

Effect of probenecid on phagocytosis and intracellular killing of Staphylococcus aureus and Escherichia coli by human monocytes and granulocytes.

H P Buisman 1, L F Buys 1, J A Langermans 1, P J van den Broek 1, R van Furth 1
PMCID: PMC1384615  PMID: 1748482

Abstract

The present study concerns the effects of probenecid on the phagocytosis and intracellular killing of Staphylococcus aureus and Escherichia coli by human monocytes and granulocytes. In both monocytes and granulocytes the inhibitory effect on phagocytosis was very small. Inhibition of intracellular killing of S. aureus by monocytes and granulocytes by probenecid was concentration dependent, being half-maximal at about 2 mM probenecid, and near-maximal at about 5 mM probenecid. The intracellular killing could also be inhibited when probenecid was added when this process was already started. Probenecid also inhibited the intracellular killing of E. coli by granulocytes, but not by monocytes. In the concentration range used, probenecid had no toxic effect on phagocytes or bacteria during the 2 hr of the experiments.

Full text

PDF
338

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blomstedt J. W., Aronson P. S. pH gradient-stimulated transport of urate and p-aminohippurate in dog renal microvillus membrane vesicles. J Clin Invest. 1980 Apr;65(4):931–934. doi: 10.1172/JCI109748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cunningham R. F., Israili Z. H., Dayton P. G. Clinical pharmacokinetics of probenecid. Clin Pharmacokinet. 1981 Mar-Apr;6(2):135–151. doi: 10.2165/00003088-198106020-00004. [DOI] [PubMed] [Google Scholar]
  3. Di Virgilio F., Steinberg T. H., Swanson J. A., Silverstein S. C. Fura-2 secretion and sequestration in macrophages. A blocker of organic anion transport reveals that these processes occur via a membrane transport system for organic anions. J Immunol. 1988 Feb 1;140(3):915–920. [PubMed] [Google Scholar]
  4. Guggino S. E., Martin G. J., Aronson P. S. Specificity and modes of the anion exchanger in dog renal microvillus membranes. Am J Physiol. 1983 Jun;244(6):F612–F621. doi: 10.1152/ajprenal.1983.244.6.F612. [DOI] [PubMed] [Google Scholar]
  5. Gutman A. B. Uricosuric drugs, with special reference to probenecid and sulfinpyrazone. Adv Pharmacol. 1966;4:91–142. doi: 10.1016/s1054-3589(08)60098-8. [DOI] [PubMed] [Google Scholar]
  6. Kahn A. M., Weinman E. J. Urate transport in the proximal tubule: in vivo and vesicle studies. Am J Physiol. 1985 Dec;249(6 Pt 2):F789–F798. doi: 10.1152/ajprenal.1985.249.6.F789. [DOI] [PubMed] [Google Scholar]
  7. Leijh P. C., van den Barselaar M. T., Dubbeldeman-Rempt I., van Furth R. Kinetics of intracellular killing of Staphylococcus aureus and Escherichia coli by human granulocytes. Eur J Immunol. 1980 Oct;10(10):750–757. doi: 10.1002/eji.1830101005. [DOI] [PubMed] [Google Scholar]
  8. Leijh P. C., van den Barselaar M. T., van Furth R. Kinetics of phagocytosis and intracellular killing of Staphylococcus aureus and Escherichia coli by human monocytes. Scand J Immunol. 1981;13(2):159–174. doi: 10.1111/j.1365-3083.1981.tb00122.x. [DOI] [PubMed] [Google Scholar]
  9. Leijh P. C., van den Barselaar M. T., van Zwet T. L., Daha M. R., van Furth R. Requirement of extracellular complement and immunoglobulin for intracellular killing of micro-organisms by human monocytes. J Clin Invest. 1979 Apr;63(4):772–784. doi: 10.1172/JCI109362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leijh P. C., van den Barselaar M. T., van Zwet T. L., Dubbeldeman-Rempt I., van Furth R. Kinetics of phagocytosis of Staphylococcus aureus and Escherichia coli by human granulocytes. Immunology. 1979 Jun;37(2):453–465. [PMC free article] [PubMed] [Google Scholar]
  11. Root R. K., Metcalf J., Oshino N., Chance B. H2O2 release from human granulocytes during phagocytosis. I. Documentation, quantitation, and some regulating factors. J Clin Invest. 1975 May;55(5):945–955. doi: 10.1172/JCI108024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Steinberg T. H., Newman A. S., Swanson J. A., Silverstein S. C. Macrophages possess probenecid-inhibitable organic anion transporters that remove fluorescent dyes from the cytoplasmic matrix. J Cell Biol. 1987 Dec;105(6 Pt 1):2695–2702. doi: 10.1083/jcb.105.6.2695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Steinberg T. H., Swanson J. A., Silverstein S. C. A prelysosomal compartment sequesters membrane-impermeant fluorescent dyes from the cytoplasmic matrix of J774 macrophages. J Cell Biol. 1988 Sep;107(3):887–896. doi: 10.1083/jcb.107.3.887. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES