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. 1991 Nov;74(3):467–472.

Murine immune response to HIV-1 p24 core protein following subcutaneous, intraperitoneal and intravenous immunization.

L Fyfe 1, J Maingay 1, A C Robinson 1, S E Howie 1
PMCID: PMC1384641  PMID: 1769693

Abstract

The murine immune response to baculovirus-produced human immunodeficiency virus type-1 (HIV-1)p24 was examined after injection by three different routes: subcutaneously (s.c.), intraperitoneally (i.p.) and intravenously (i.v.). Both antigen-specific T-cell proliferation and serum antibody were induced by i.p. injection. In contrast, s.c. and i.v. injection of antigen resulted in specific antibody generation alone. Lympho-proliferative responses seen after i.p. injection were confined to splenocytes, and were greater after a low dose of antigen than after a high dose. p24-specific proliferation was not detected in lymph node cells. CD4:CD8 ratios were normal in lymph nodes and spleen at all times, irrespective of the dose or route of administration. p24-specific serum IgG antibodies were detected in all animals after the second injection of antigen. After s.c. and i.v. administration of high doses of antigen, the median antibody titres continued to rise after a third injection, but plateaued in animals injected by the i.p. route. In contrast, low doses of antigen given i.p. increased the median titre during and after the course of four injections. A low antigen dose given s.c. resulted in a plateau of median titre between the third and fourth injections. In i.v.-injected animals the median titre decreased between the third and fourth injections. IgG1 p24-specific antibodies were detected in all immunized mice, whereas IgM antibodies were detectable only following i.p. injection of antigen.

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Selected References

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  1. Amadori A., Chieco-Bianchi L. B-cell activation and HIV-1 infection: deeds and misdeeds. Immunol Today. 1990 Oct;11(10):374–379. doi: 10.1016/0167-5699(90)90144-x. [DOI] [PubMed] [Google Scholar]
  2. Cobbold S. P., Jayasuriya A., Nash A., Prospero T. D., Waldmann H. Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo. Nature. 1984 Dec 6;312(5994):548–551. doi: 10.1038/312548a0. [DOI] [PubMed] [Google Scholar]
  3. Fauci A. S. The human immunodeficiency virus: infectivity and mechanisms of pathogenesis. Science. 1988 Feb 5;239(4840):617–622. doi: 10.1126/science.3277274. [DOI] [PubMed] [Google Scholar]
  4. Iwarson S., Tabor E., Thomas H. C., Snoy P., Gerety R. J. Protection against hepatitis B virus infection by immunization with hepatitis B core antigen. Gastroenterology. 1985 Mar;88(3):763–767. doi: 10.1016/0016-5085(85)90148-9. [DOI] [PubMed] [Google Scholar]
  5. Lange J. M., Paul D. A., Huisman H. G., de Wolf F., van den Berg H., Coutinho R. A., Danner S. A., van der Noordaa J., Goudsmit J. Persistent HIV antigenaemia and decline of HIV core antibodies associated with transition to AIDS. Br Med J (Clin Res Ed) 1986 Dec 6;293(6560):1459–1462. doi: 10.1136/bmj.293.6560.1459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mills H. R., Jones I. M. Expression and purification of p24, the core protein of HIV, using a baculovirus-insect cell expression system. AIDS. 1990 Nov;4(11):1125–1131. doi: 10.1097/00002030-199011000-00011. [DOI] [PubMed] [Google Scholar]
  7. Mitchison N. A. Suppression. Immunol Today. 1989 Dec;10(12):392–393. doi: 10.1016/0167-5699(89)90028-5. [DOI] [PubMed] [Google Scholar]
  8. Murray K., Bruce S. A., Wingfield P., van Eerd P., de Reus A., Schellekens H. Protective immunisation against hepatitis B with an internal antigen of the virus. J Med Virol. 1987 Oct;23(2):101–107. doi: 10.1002/jmv.1890230202. [DOI] [PubMed] [Google Scholar]
  9. Möller G., Bergstedt S., Dai S., Fernandez C., Möller E., Ramos T. The degree of clonal elimination in various types of specific immunological unresponsiveness. Ann N Y Acad Sci. 1982;392:23–34. doi: 10.1111/j.1749-6632.1982.tb36095.x. [DOI] [PubMed] [Google Scholar]
  10. Ptak W., Rózycka D., Rewicka M. Induction of suppressor cells and cells producing antigen-specific suppressor factors by haptens bound to self carriers. Immunobiology. 1980 Jan;156(4-5):400–409. doi: 10.1016/S0171-2985(80)80073-8. [DOI] [PubMed] [Google Scholar]
  11. Russell S. M., Liew F. Y. Cell cooperation in antibody responses to influenza virus. I. priming of helper t cells by internal components of virion. Eur J Immunol. 1980 Oct;10(10):791–796. doi: 10.1002/eji.1830101013. [DOI] [PubMed] [Google Scholar]
  12. Schüpbach J., Haller O., Vogt M., Lüthy R., Joller H., Oelz O., Popovic M., Sarngadharan M. G., Gallo R. C. Antibodies to HTLV-III in Swiss patients with AIDS and pre-AIDS and in groups at risk for AIDS. N Engl J Med. 1985 Jan 31;312(5):265–270. doi: 10.1056/NEJM198501313120502. [DOI] [PubMed] [Google Scholar]
  13. Weber J. N., Clapham P. R., Weiss R. A., Parker D., Roberts C., Duncan J., Weller I., Carne C., Tedder R. S., Pinching A. J. Human immunodeficiency virus infection in two cohorts of homosexual men: neutralising sera and association of anti-gag antibody with prognosis. Lancet. 1987 Jan 17;1(8525):119–122. doi: 10.1016/s0140-6736(87)91964-7. [DOI] [PubMed] [Google Scholar]
  14. Yarchoan R., Mitsuya H., Broder S. Immunologic issues in anti-retroviral therapy. Immunol Today. 1990 Sep;11(9):327–333. doi: 10.1016/0167-5699(90)90128-v. [DOI] [PubMed] [Google Scholar]

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