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. 1991 Nov;74(3):546–551.

Intestinal mucus entrapment of Trichinella spiralis larvae induced by specific antibodies.

M S Carlisle 1, D D McGregor 1, J A Appleton 1
PMCID: PMC1384653  PMID: 1769701

Abstract

Entrapment of muscle larvae (ML) occurred in vitro when antibodies specific for Trichinella spiralis were added directly to intestinal mucus from normal non-immunized rats or when mucus was collected from pups suckling a T. spiralis-infected dam. Normal rat serum immunoglobulins failed to promote mucus entrapment and complement did not appear to play a part in the entrapment process. Differences were not observed in the efficiency of entrapment of ML by mucus harvested from different regions of the small intestine. Employing a panel of monoclonal antibodies (mAb) specific for excretory-secretory antigen (ESA), we attempted to dissociate antibody-mediated protection from mucus entrapment. We assessed mucus entrapment and rapid expulsion by these mAb in vivo, and observed protection in the absence of significant, immediate mucus entrapment in two cases. In addition, we measured mucus entrapment of ML in two in vitro assays. One assay employed intestinal mucus harvested from pups suckling dams that had been injected i.v. with a mAb. Results confirmed those obtained in vivo and indicated that antibodies were present in the intestinal lumina of passively immunized pups. In the second in vitro assay, mAb were added individually to mucus from pups suckling non-immunized dams. Results from these assays suggested that certain antibody isotypes may be processed in vivo in ways that influence, either positively or negatively, their abilities to cause mucus entrapment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleton J. A., McGregor D. D. Characterization of the immune mediator of rapid expulsion of Trichinella spiralis in suckling rats. Immunology. 1987 Nov;62(3):477–484. [PMC free article] [PubMed] [Google Scholar]
  2. Appleton J. A., McGregor D. D. Life-phase specific induction and expression of rapid expulsion in rats suckling Trichinella spiralis-infected dams. Immunology. 1985 Jun;55(2):225–232. [PMC free article] [PubMed] [Google Scholar]
  3. Appleton J. A., Schain L. R., McGregor D. D. Rapid expulsion of Trichinella spiralis in suckling rats: mediation by monoclonal antibodies. Immunology. 1988 Nov;65(3):487–492. [PMC free article] [PubMed] [Google Scholar]
  4. Bell R. G., Adams L. S., Ogden R. W. Intestinal mucus trapping in the rapid expulsion of Trichinella spiralis by rats: induction and expression analyzed by quantitative worm recovery. Infect Immun. 1984 Jul;45(1):267–272. doi: 10.1128/iai.45.1.267-272.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Borthistle B. K., Kubo R. T., Brown W. R., Grey H. M. Studies on receptors for IgG on epithelial cells of the rat intestine. J Immunol. 1977 Aug;119(2):471–476. [PubMed] [Google Scholar]
  6. Carlisle M. S., McGregor D. D., Appleton J. A. The role of mucus in antibody-mediated rapid expulsion of Trichinella spiralis in suckling rats. Immunology. 1990 May;70(1):126–132. [PMC free article] [PubMed] [Google Scholar]
  7. Carlisle M. S., McGregor D. D., Appleton J. A. The role of the antibody Fc region in rapid expulsion of Trichinella spiralis in suckling rats. Immunology. 1991 Nov;74(3):552–558. [PMC free article] [PubMed] [Google Scholar]
  8. Douglass T. G., Speer C. A. Effects of intestinal contents from normal and immunized mice on sporozoites of Eimeria falciformis. J Protozool. 1985 Feb;32(1):156–163. doi: 10.1111/j.1550-7408.1985.tb03031.x. [DOI] [PubMed] [Google Scholar]
  9. Jass J. R. Role of intestinal metaplasia in the histogenesis of gastric carcinoma. J Clin Pathol. 1980 Sep;33(9):801–810. doi: 10.1136/jcp.33.9.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miller H. R., Huntley J. F., Wallace G. R. Immune exclusion and mucus trapping during the rapid expulsion of Nippostrongylus brasiliensis from primed rats. Immunology. 1981 Oct;44(2):419–429. [PMC free article] [PubMed] [Google Scholar]
  11. Miller H. R., Jackson F., Newlands G., Appleyard W. T. Immune exclusion, a mechanism of protection against the ovine nematode Haemonchus contortus. Res Vet Sci. 1983 Nov;35(3):357–363. [PubMed] [Google Scholar]
  12. Munster D. J., Bagshaw P. F., Wilson J. G. Peptic erosion of gastric mucus in the rat. Comp Biochem Physiol A Comp Physiol. 1987;87(2):509–513. doi: 10.1016/0300-9629(87)90159-9. [DOI] [PubMed] [Google Scholar]
  13. Sarosiek J., Slomiany A., Slomiany B. L. Evidence for weakening of gastric mucus integrity by Campylobacter pylori. Scand J Gastroenterol. 1988 Jun;23(5):585–590. doi: 10.3109/00365528809093916. [DOI] [PubMed] [Google Scholar]
  14. Schrank G. D., Verwey W. F. Distribution of cholera organisms in experimental Vibrio cholerae infections: proposed mechanisms of pathogenesis and antibacterial immunity. Infect Immun. 1976 Jan;13(1):195–203. doi: 10.1128/iai.13.1.195-203.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wesley A., Mantle M., Man D., Qureshi R., Forstner G., Forstner J. Neutral and acidic species of human intestinal mucin. Evidence for different core peptides. J Biol Chem. 1985 Jul 5;260(13):7955–7959. [PubMed] [Google Scholar]
  16. Younan F., Pearson J., Allen A., Venables C. Changes in the structure of the mucous gel on the mucosal surface of the stomach in association with peptic ulcer disease. Gastroenterology. 1982 May;82(5 Pt 1):827–831. [PubMed] [Google Scholar]

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