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. 1992 Mar;75(3):528–534.

Precipitated immune complexes of IgM as well as of IgG can bind to rabbit polymorphonuclear leucocytes but only the immune complexes of IgG are readily phagocytosed.

R P Furriel 1, Y M Lucisano 1, B Mantovani 1
PMCID: PMC1384750  PMID: 1572698

Abstract

We have shown by in vitro experiments, using immunofluorescence techniques, that precipitated immune complexes of IgM antibodies and ovalbumin (ICIgM) are able to bind to rabbit blood polymorphonuclear leucocytes (PMN), as well as immune complexes of IgG antibodies (ICIgG). This binding capacity for both classes of immune complexes is exhibited by more than 80% of the PMN cell population and is independent of Ca2+ in the medium. For ICIgG the binding to PMN can be completely inhibited by preincubation of the cells with soluble IgG used at physiological concentrations (competition for the Fc gamma receptors) while for ICIgM there is no such inhibition by fluid-phase IgM. After binding to the leucocytes there was a striking difference in the fate of ICIgM and ICIgG: whereas the ICIgG was readily phagocytosed (endocytosed), the ICIgM remained mostly on the cell surface, being only poorly endocytosed after 1 hr incubation at 37 degrees. This was demonstrated by a quantitative fluorimetric method developed to assay phagocytosis of immune complexes, and was confirmed by a qualitative fluorescence quenching technique. These results may have implications for understanding the fate of these classes of immune complexes formed in circulation or deposited in tissues, and the participation of PMN in inflammatory reactions and tissue injury in immune complex diseases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burton D. R. Immunoglobulin G: functional sites. Mol Immunol. 1985 Mar;22(3):161–206. doi: 10.1016/0161-5890(85)90151-8. [DOI] [PubMed] [Google Scholar]
  2. CLARK H. F., SHEPARD C. C. A DIALYSIS TECHNIQUE FOR PREPARING FLUORESCENT ANTIBODY. Virology. 1963 Aug;20:642–644. doi: 10.1016/0042-6822(63)90292-7. [DOI] [PubMed] [Google Scholar]
  3. Epstein E. H., Jr, Scott R. D., Miller E. J., Piez K. A. Isolation and characterization of the peptides derived from soluble human and baboon skin collagen after cyanogen bromide cleavage. J Biol Chem. 1971 Mar 25;246(6):1718–1724. [PubMed] [Google Scholar]
  4. Fantone J. C., Ward P. A. Polymorphonuclear leukocyte-mediated cell and tissue injury: oxygen metabolites and their relations to human disease. Hum Pathol. 1985 Oct;16(10):973–978. doi: 10.1016/s0046-8177(85)80273-2. [DOI] [PubMed] [Google Scholar]
  5. Fantone J. C., Ward P. A. Role of oxygen-derived free radicals and metabolites in leukocyte-dependent inflammatory reactions. Am J Pathol. 1982 Jun;107(3):395–418. [PMC free article] [PubMed] [Google Scholar]
  6. GMRDON M. A., EDWARDS M. R., TOMPKINS V. N. Refinement of fluorescent antibody by gel filtration. Proc Soc Exp Biol Med. 1962 Jan;109:96–99. doi: 10.3181/00379727-109-27114. [DOI] [PubMed] [Google Scholar]
  7. Grewal A. S., Rouse B. T., Babiuk L. A. Characterization of surface receptors on bovine leukocytes. Int Arch Allergy Appl Immunol. 1978;56(4):289–300. doi: 10.1159/000232034. [DOI] [PubMed] [Google Scholar]
  8. Hed J. Methods for distinguishing ingested from adhering particles. Methods Enzymol. 1986;132:198–204. doi: 10.1016/s0076-6879(86)32008-1. [DOI] [PubMed] [Google Scholar]
  9. Henson P. M., Johnson H. B., Spiegelberg H. L. The release of granule enzymes from human neutrophils stimulated by aggregated immunoglobulins of different classes and subclasses. J Immunol. 1972 Dec;109(6):1182–1192. [PubMed] [Google Scholar]
  10. Henson P. M. Pathologic mechanisms in neutrophil-mediated injury. Am J Pathol. 1972 Sep;68(3):593–612. [PMC free article] [PubMed] [Google Scholar]
  11. Henson P. M. The adherence of leucocytes and platelets induced by fixed IgG antibody or complement. Immunology. 1969 Jan;16(1):107–121. [PMC free article] [PubMed] [Google Scholar]
  12. ITZHAKI R. F., GILL D. M. A MICRO-BIURET METHOD FOR ESTIMATING PROTEINS. Anal Biochem. 1964 Dec;9:401–410. doi: 10.1016/0003-2697(64)90200-3. [DOI] [PubMed] [Google Scholar]
  13. Kurlander R. J., Batker J. The binding of human immunoglobulin G1 monomer and small, covalently cross-linked polymers of immunoglobulin G1 to human peripheral blood monocytes and polymorphonuclear leukocytes. J Clin Invest. 1982 Jan;69(1):1–8. doi: 10.1172/JCI110419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lawrence D. A., Weigle W. O., Spiegelberg H. L. Immunoglobulins cytophilic for human lymphocytes, monocytes, and neutrophils. J Clin Invest. 1975 Feb;55(2):368–376. doi: 10.1172/JCI107940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lay W. H., Nussenzweig V. Ca++-dependent binding of antigen-19 S antibody complexes to macrophages. J Immunol. 1969 May;102(5):1172–1178. [PubMed] [Google Scholar]
  16. Lay W. H., Nussenzweig V. Receptors for complement of leukocytes. J Exp Med. 1968 Nov 1;128(5):991–1009. doi: 10.1084/jem.128.5.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lucisano Y. M., Mantovani B. Lysosomal enzyme release from polymorphonuclear leukocytes induced by immune complexes of IgM and of IgG. J Immunol. 1984 Apr;132(4):2015–2020. [PubMed] [Google Scholar]
  18. Mantovani B. Different roles of IgG and complement receptors in phagocytosis by polymorphonuclear leukocytes. J Immunol. 1975 Jul;115(1):15–17. [PubMed] [Google Scholar]
  19. Mantovani B., Rabinovitch M., Nussenzweig V. Phagocytosis of immune complexes by macrophages. Different roles of the macrophage receptor sites for complement (C3) and for immunoglobulin (IgG). J Exp Med. 1972 Apr 1;135(4):780–792. doi: 10.1084/jem.135.4.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ohno T., Kubagawa H., Sanders S. K., Cooper M. D. Biochemical nature of an Fc mu receptor on human B-lineage cells. J Exp Med. 1990 Oct 1;172(4):1165–1175. doi: 10.1084/jem.172.4.1165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shen L., Guyre P. M., Fanger M. W. Polymorphonuclear leukocyte function triggered through the high affinity Fc receptor for monomeric IgG. J Immunol. 1987 Jul 15;139(2):534–538. [PubMed] [Google Scholar]
  22. Tejani A., Nicastri A. D. Mesangial IgM nephropathy. Nephron. 1983;35(1):1–5. doi: 10.1159/000183035. [DOI] [PubMed] [Google Scholar]
  23. Tenner A. J., Cooper N. R. Analysis of receptor-mediated C1q binding to human peripheral blood mononuclear cells. J Immunol. 1980 Oct;125(4):1658–1664. [PubMed] [Google Scholar]
  24. Tenner A. J., Cooper N. R. Stimulation of a human polymorphonuclear leukocyte oxidative response by the C1q subunit of the first complement component. J Immunol. 1982 Jun;128(6):2547–2552. [PubMed] [Google Scholar]
  25. Walsh G. M., Kay A. B. Binding of immunoglobulin classes and subclasses to human neutrophils and eosinophils. Clin Exp Immunol. 1986 Feb;63(2):466–472. [PMC free article] [PubMed] [Google Scholar]
  26. Ward P. A., Zvaifler N. J. Quantitative phagocytosis by neutrophils. I. A new method with immune complexes. J Immunol. 1973 Dec;111(6):1771–1776. [PubMed] [Google Scholar]
  27. Yonemasu K., Stroud R. M. Clq: rapid purification method for preparation of monospecific antisera and for biochemical studies. J Immunol. 1971 Feb;106(2):304–313. [PubMed] [Google Scholar]

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