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. 1992 Mar;75(3):550–552.

Major immunoglobulin capping deficiency in the peripheral blood B cells of patients with Sjögren's syndrome.

R Bataille 1, X G Zhang 1, C Duperray 1, G Rajzbaum 1, J Brochier 1, B Klein 1
PMCID: PMC1384754  PMID: 1572701

Abstract

The capping of surface immunoglobulins (sIg) is a major characteristic of normal B lymphocytes. Thus, we have investigated sIg capping by peripheral blood (PB) B cells of patients with Sjögren's syndrome (SS) and we have found a major deficiency in these patients. In 12 healthy donors (HD), 8 +/- 2.8% of PB mononuclear cells were B cells (i.e. expressing the B-cell antigens CD19, CD20 and CD21 simultaneously) and more than 90% of these PB B cells were able to cap their sIg. In 12 experiments performed using PB lymphocytes from seven patients with SS, a major capping deficiency was noted with only 30% of PB B lymphocytes being able to cap sIg. This defect was not related to an expansion of the B-cell subpopulation expressing the CD5 antigen and was not observed in five patients with rheumatoid arthritis lacking SS. Capping of sIg via antigen binding (i.e. antigenic modulation) constitutes the initial signal for B-cell activation. This process is involved in anti-viral defence and could have a potential pathogenetic role in autoimmune diseases. This impaired B-cell function presently described represents an immune defect which could be important in the pathogenesis of SS.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cohen H. J. Human lymphocyte surface immunoglobulin capping. Normal characteristics and anomalous behavior of chronic lymphocytic leukemic lymphocytes. J Clin Invest. 1975 Jan;55(1):84–93. doi: 10.1172/JCI107921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dauphinée M., Tovar Z., Talal N. B cells expressing CD5 are increased in Sjögren's syndrome. Arthritis Rheum. 1988 May;31(5):642–647. doi: 10.1002/art.1780310509. [DOI] [PubMed] [Google Scholar]
  3. Fox R. I., Robinson C. A., Curd J. G., Kozin F., Howell F. V. Sjögren's syndrome. Proposed criteria for classification. Arthritis Rheum. 1986 May;29(5):577–585. doi: 10.1002/art.1780290501. [DOI] [PubMed] [Google Scholar]
  4. Graziadei L., Riabowol K., Bar-Sagi D. Co-capping of ras proteins with surface immunoglobulins in B lymphocytes. Nature. 1990 Sep 27;347(6291):396–400. doi: 10.1038/347396a0. [DOI] [PubMed] [Google Scholar]
  5. Hardy R. R., Hayakawa K., Shimizu M., Yamasaki K., Kishimoto T. Rheumatoid factor secretion from human Leu-1+ B cells. Science. 1987 Apr 3;236(4797):81–83. doi: 10.1126/science.3105057. [DOI] [PubMed] [Google Scholar]
  6. Kumagai K., Abo T., Sekizawa T., Sasaki M. Studies of surface immunoglobulins on human B lymphocytes. I. Dissociation of cell-bound immunoglobulins with acid pH or at 37 degrees C. J Immunol. 1975 Oct;115(4):982–987. [PubMed] [Google Scholar]
  7. Unanue E. R., Karnovsky M. J. Redistribution and fate of Ig complexes on surface of B lymphocytes: functional implications and mechanisms. Transplant Rev. 1973;14:184–210. doi: 10.1111/j.1600-065x.1973.tb00107.x. [DOI] [PubMed] [Google Scholar]
  8. Youinou P., Mackenzie L., le Masson G., Papadopoulos N. M., Jouquan J., Pennec Y. L., Angelidis P., Katsikis P., Moutsopoulos H. M., Lydyard P. M. CD5-expressing B lymphocytes in the blood and salivary glands of patients with primary Sjögren's syndrome. J Autoimmun. 1988 Apr;1(2):185–194. doi: 10.1016/0896-8411(88)90025-x. [DOI] [PubMed] [Google Scholar]
  9. Zhang X. G., Klein B., Duperray C., Brochier J., Bataille R. Delay and not deficiency in cap formation of peripheral blood B cells in patients with multiple myeloma. J Clin Immunol. 1988 Jul;8(4):244–249. doi: 10.1007/BF00916552. [DOI] [PubMed] [Google Scholar]

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