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. 1991 Dec;74(4):680–684.

Conglutinin exhibits a complement-dependent enhancement of the respiratory burst of phagocytes stimulated by E. coli.

P Friis 1, S E Svehag 1, O Andersen 1, B Gahrn-Hansen 1, R G Leslie 1
PMCID: PMC1384779  PMID: 1783427

Abstract

Conglutinin is a mammalian C-type lectin which shows anti-bacterial activity when tested in vivo and in vitro. This study concerns the effect of conglutinin on the respiratory burst of murine spleen cells, using a chemiluminescence assay for measurement of generated reactive oxygen metabolites. Conglutinin enhances, in a dose-dependent manner, the respiratory burst of spleen cells stimulated with serum-opsonized Escherichia coli. The enhancement was only demonstrable in the presence of a functional complement system. The conglutinin-mediated enhancement of the respiratory burst was inhibited in the presence of a N-acetyl-D-glucosamine, D-mannose and N-acetyl-D-mannosamine, monosaccharides reported to inhibit conglutinin-binding to zymosan and the complement factor iC3b. On the other hand, N-acetyl-D-galactosamine was non-inhibitory.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baatrup G., Thiel S., Isager H., Svehag S. E., Jensenius J. C. Demonstration in human plasma of a lectin activity analogous to that of bovine conglutinin. Scand J Immunol. 1987 Oct;26(4):355–361. doi: 10.1111/j.1365-3083.1987.tb02267.x. [DOI] [PubMed] [Google Scholar]
  2. Bobak D. A., Gaither T. A., Frank M. M., Tenner A. J. Modulation of FcR function by complement: subcomponent C1q enhances the phagocytosis of IgG-opsonized targets by human monocytes and culture-derived macrophages. J Immunol. 1987 Feb 15;138(4):1150–1156. [PubMed] [Google Scholar]
  3. Bobak D. A., Washburn R. G., Frank M. M. C1q enhances the phagocytosis of Cryptococcus neoformans blastospores by human monocytes. J Immunol. 1988 Jul 15;141(2):592–597. [PubMed] [Google Scholar]
  4. Drickamer K. Two distinct classes of carbohydrate-recognition domains in animal lectins. J Biol Chem. 1988 Jul 15;263(20):9557–9560. [PubMed] [Google Scholar]
  5. Friis-Christiansen P., Thiel S., Svehag S. E., Dessau R., Svendsen P., Andersen O., Laursen S. B., Jensenius J. C. In vivo and in vitro antibacterial activity of conglutinin, a mammalian plasma lectin. Scand J Immunol. 1990 Apr;31(4):453–460. doi: 10.1111/j.1365-3083.1990.tb02792.x. [DOI] [PubMed] [Google Scholar]
  6. Hirani S., Lambris J. D., Müller-Eberhard H. J. Localization of the conglutinin binding site on the third component of human complement. J Immunol. 1985 Feb;134(2):1105–1109. [PubMed] [Google Scholar]
  7. Kuhlman M., Joiner K., Ezekowitz R. A. The human mannose-binding protein functions as an opsonin. J Exp Med. 1989 May 1;169(5):1733–1745. doi: 10.1084/jem.169.5.1733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lee Y. M., Leiby K. R., Allar J., Paris K., Lerch B., Okarma T. B. Primary structure of bovine conglutinin, a member of the C-type animal lectin family. J Biol Chem. 1991 Feb 15;266(5):2715–2723. [PubMed] [Google Scholar]
  9. Loveless R. W., Feizi T., Childs R. A., Mizuochi T., Stoll M. S., Oldroyd R. G., Lachmann P. J. Bovine serum conglutinin is a lectin which binds non-reducing terminal N-acetylglucosamine, mannose and fucose residues. Biochem J. 1989 Feb 15;258(1):109–113. doi: 10.1042/bj2580109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Malhotra R., Thiel S., Reid K. B., Sim R. B. Human leukocyte C1q receptor binds other soluble proteins with collagen domains. J Exp Med. 1990 Sep 1;172(3):955–959. doi: 10.1084/jem.172.3.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rasmussen J. M., Teisner B., Brandt J., Brandslund I., Gry H. Metabolism of C3 and factor B in patients with congenital factor I deficiency. J Clin Lab Immunol. 1990 Feb;31(2):59–67. [PubMed] [Google Scholar]
  12. Rest R. F., Farrell C. F., Naids F. L. Mannose inhibits the human neutrophil oxidative burst. J Leukoc Biol. 1988 Feb;43(2):158–164. doi: 10.1002/jlb.43.2.158. [DOI] [PubMed] [Google Scholar]
  13. Teisner B., Elling P., Svehag S. E., Poulsen L., Lamm L. U., Sjöholm A. C3 nephritic factor in a patient with recurrent Neisseria meningitidis infections. Acta Pathol Microbiol Immunol Scand C. 1984 Dec;92(6):341–349. doi: 10.1111/j.1699-0463.1984.tb00098.x. [DOI] [PubMed] [Google Scholar]
  14. Tenner A. J., Cooper N. R. Stimulation of a human polymorphonuclear leukocyte oxidative response by the C1q subunit of the first complement component. J Immunol. 1982 Jun;128(6):2547–2552. [PubMed] [Google Scholar]
  15. van Iwaarden F., Welmers B., Verhoef J., Haagsman H. P., van Golde L. M. Pulmonary surfactant protein A enhances the host-defense mechanism of rat alveolar macrophages. Am J Respir Cell Mol Biol. 1990 Jan;2(1):91–98. doi: 10.1165/ajrcmb/2.1.91. [DOI] [PubMed] [Google Scholar]

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