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. 1992 Jan;75(1):202–205.

Expression of the CD46 antigen, and absence of class I MHC antigen, on the human oocyte and preimplantation blastocyst.

J M Roberts 1, C T Taylor 1, G C Melling 1, C R Kingsland 1, P M Johnson 1
PMCID: PMC1384825  PMID: 1632879

Abstract

Expression of CD46 and class I major histocompatibility complex (MHC) antigens by human oocytes and 6-8-day unhatched expanded preimplantation blastocysts has been studied by immunocytochemistry. The CD46 antigen, a cell surface complement regulatory protein, was expressed by unfertilized oocytes as well as strongly by both the inner cell mass and trophectoderm of preimplantation blastocysts. In contrast, class I MHC antigens were not usually expressed by either oocytes or blastocysts. These data support the concept that gametes and embryonic cells involved in fertilization and early implantation events, respectively, may be protected from immunological recognition or attack both by the lack of class I MHC antigens and by expression of the CD46 complement regulatory protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. J., Bach D. L., Yunis E. J., DeWolf W. C. Major histocompatibility antigens are not expressed on human epididymal sperm. J Immunol. 1982 Aug;129(2):452–454. [PubMed] [Google Scholar]
  2. Anderson D. J., Michaelson J. S., Johnson P. M. Trophoblast/leukocyte-common antigen is expressed by human testicular germ cells and appears on the surface of acrosome-reacted sperm. Biol Reprod. 1989 Aug;41(2):285–293. doi: 10.1095/biolreprod41.2.285. [DOI] [PubMed] [Google Scholar]
  3. Barnstable C. J., Bodmer W. F., Brown G., Galfre G., Milstein C., Williams A. F., Ziegler A. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell. 1978 May;14(1):9–20. doi: 10.1016/0092-8674(78)90296-9. [DOI] [PubMed] [Google Scholar]
  4. Bedford J. M., Witkin S. S. Influence of complement depletion on sperm function in the female rabbit. J Reprod Fertil. 1983 Nov;69(2):523–528. doi: 10.1530/jrf.0.0690523. [DOI] [PubMed] [Google Scholar]
  5. Braude P., Bolton V., Moore S. Human gene expression first occurs between the four- and eight-cell stages of preimplantation development. Nature. 1988 Mar 31;332(6163):459–461. doi: 10.1038/332459a0. [DOI] [PubMed] [Google Scholar]
  6. Cho S. W., Oglesby T. J., Hsi B. L., Adams E. M., Atkinson J. P. Characterization of three monoclonal antibodies to membrane co-factor protein (MCP) of the complement system and quantification of MCP by radioassay. Clin Exp Immunol. 1991 Feb;83(2):257–261. doi: 10.1111/j.1365-2249.1991.tb05624.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Desoye G., Dohr G. A., Motter W., Winter R., Urdl W., Pusch H., Uchanska-Ziegler B., Ziegler A. Lack of HLA class I and class II antigens on human preimplantation embryos. J Immunol. 1988 Jun 15;140(12):4157–4159. [PubMed] [Google Scholar]
  8. Dohr G. HLA and TLX antigen expression on the human oocyte, zona pellucida and granulosa cells. Hum Reprod. 1987 Nov;2(8):657–664. doi: 10.1093/oxfordjournals.humrep.a136611. [DOI] [PubMed] [Google Scholar]
  9. Ellis S. A., Palmer M. S., McMichael A. J. Human trophoblast and the choriocarcinoma cell line BeWo express a truncated HLA Class I molecule. J Immunol. 1990 Jan 15;144(2):731–735. [PubMed] [Google Scholar]
  10. Hourcade D., Holers V. M., Atkinson J. P. The regulators of complement activation (RCA) gene cluster. Adv Immunol. 1989;45:381–416. doi: 10.1016/s0065-2776(08)60697-5. [DOI] [PubMed] [Google Scholar]
  11. Isaacson K. B., Galman M., Coutifaris C., Lyttle C. R. Endometrial synthesis and secretion of complement component-3 by patients with and without endometriosis. Fertil Steril. 1990 May;53(5):836–841. [PubMed] [Google Scholar]
  12. Johnson P. M., Bulmer J. N. Uterine gland epithelium in human pregnancy often lacks detectable maternal MHC antigens but does express fetal trophoblast antigens. J Immunol. 1984 Apr;132(4):1608–1610. [PubMed] [Google Scholar]
  13. Kingsland C. R., Taylor C. T., Aziz N., Bickerton N. Is follicular flushing necessary for oocyte retrieval? A randomized trial. Hum Reprod. 1991 Mar;6(3):382–383. doi: 10.1093/oxfordjournals.humrep.a137344. [DOI] [PubMed] [Google Scholar]
  14. Kovats S., Main E. K., Librach C., Stubblebine M., Fisher S. J., DeMars R. A class I antigen, HLA-G, expressed in human trophoblasts. Science. 1990 Apr 13;248(4952):220–223. doi: 10.1126/science.2326636. [DOI] [PubMed] [Google Scholar]
  15. McLaughlin P. J., Cheng M. H., Slade M. B., Johnson P. M. Expression on cultured human tumour cells of placental trophoblast membrane antigens and placental alkaline phosphatase defined by monoclonal antibodies. Int J Cancer. 1982 Jul 15;30(1):21–26. doi: 10.1002/ijc.2910300105. [DOI] [PubMed] [Google Scholar]
  16. McNearney T., Ballard L., Seya T., Atkinson J. P. Membrane cofactor protein of complement is present on human fibroblast, epithelial, and endothelial cells. J Clin Invest. 1989 Aug;84(2):538–545. doi: 10.1172/JCI114196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Purcell D. F., McKenzie I. F., Lublin D. M., Johnson P. M., Atkinson J. P., Oglesby T. J., Deacon N. J. The human cell-surface glycoproteins HuLy-m5, membrane co-factor protein (MCP) of the complement system, and trophoblast leucocyte-common (TLX) antigen, are CD46. Immunology. 1990 Jun;70(2):155–161. [PMC free article] [PubMed] [Google Scholar]
  18. Risk J. M., Flanagan B. F., Johnson P. M. Polymorphism of the human CD46 gene in normal individuals and in recurrent spontaneous abortion. Hum Immunol. 1991 Mar;30(3):162–167. doi: 10.1016/0198-8859(91)90030-d. [DOI] [PubMed] [Google Scholar]
  19. Risk J. M., Johnson P. M. Northern blot analysis of HLA-G expression by BeWo human choriocarcinoma cells. J Reprod Immunol. 1990 Sep;18(2):199–203. doi: 10.1016/0165-0378(90)90017-z. [DOI] [PubMed] [Google Scholar]
  20. Stern P. L., Beresford N., Thompson S., Johnson P. M., Webb P. D., Hole N. Characterization of the human trophoblast-leukocyte antigenic molecules defined by a monoclonal antibody. J Immunol. 1986 Sep 1;137(5):1604–1609. [PubMed] [Google Scholar]
  21. Suarez S. S., Oliphant G. Interaction of rabbit spermatozoa and serum complement components. Biol Reprod. 1982 Sep;27(2):473–483. doi: 10.1095/biolreprod27.2.473. [DOI] [PubMed] [Google Scholar]
  22. Sundstrom S. A., Komm B. S., Ponce-de-Leon H., Yi Z., Teuscher C., Lyttle C. R. Estrogen regulation of tissue-specific expression of complement C3. J Biol Chem. 1989 Oct 5;264(28):16941–16947. [PubMed] [Google Scholar]
  23. Tesarík J., Kopecný V., Plachot M., Mandelbaum J. Early morphological signs of embryonic genome expression in human preimplantation development as revealed by quantitative electron microscopy. Dev Biol. 1988 Jul;128(1):15–20. doi: 10.1016/0012-1606(88)90261-8. [DOI] [PubMed] [Google Scholar]
  24. Webb P. D., McLaughlin P. J., Risk J. M., Johnson P. M. Isolation of placental-type alkaline phosphatase associated with human syncytiotrophoblast membranes using monoclonal antibodies. Placenta. 1986 Sep-Oct;7(5):405–415. doi: 10.1016/s0143-4004(86)80028-5. [DOI] [PubMed] [Google Scholar]

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