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. 1988 Jun;64(2):213–218.

The interaction of infant formula with macrophages: effect on phagocytic activity, relationship to expression of class II MHC antigen and survival of orally administered macrophages in the neonatal gut.

A Hughes 1, J H Brock 1, D M Parrott 1, F Cockburn 1
PMCID: PMC1384945  PMID: 3391641

Abstract

The effect of infant formula on human peritoneal and breast milk macrophages has been investigated. The ability of peritoneal macrophages to subsequently ingest and degrade immune complexes was slightly impaired, but breast milk cells were not affected. However, the cells were found to have bound antigenically intact casein and beta-lactoglobulin, although little, if any, alpha-lactalbumin was bound. Furthermore, a positive correlation was found between binding of these proteins and expression of HLA-DR antigen. Labelled macrophages fed to newborn mice survived for at least 4 hr in the gastrointestinal tract and, in some cases, localized in the mucosal tissue. In one case a labelled cell was found in the spleen. These findings indicate that breast milk macrophages may be able to perform immunological functions in the gut, and suggest that binding of cows' milk proteins by macrophages may constitute a first step in the sensitization of the neonate to cow's milk proteins. Human milk macrophages may also play a protective role by acting as antigen-presenting cells in the local immune response of the gut.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Devey M. E., Anderson K. J., Coombs R. R., Henschel M. J., Coates M. E. The modified anaphylaxis hypothesis for cot death. Anaphylactic sensitization in guinea-pigs fed cow's milk. Clin Exp Immunol. 1976 Dec;26(3):542–548. [PMC free article] [PubMed] [Google Scholar]
  2. Golder J. P., Doe W. F. Isolation and preliminary characterization of human intestinal macrophages. Gastroenterology. 1983 Apr;84(4):795–802. [PubMed] [Google Scholar]
  3. Goldstein C. S., Bomalaski J. S., Zurier R. B., Neilson E. G., Douglas S. D. Analysis of peritoneal macrophages in continuous ambulatory peritoneal dialysis patients. Kidney Int. 1984 Nov;26(5):733–740. doi: 10.1038/ki.1984.209. [DOI] [PubMed] [Google Scholar]
  4. Hughes A., Brock J. H., Parrott D. M., Cockburn F. Effect of human colostrum and infant formula on the phagocytic activity of macrophages. I. Resident and stimulated mouse peritoneal macrophages. Clin Exp Immunol. 1985 Jul;61(1):169–175. [PMC free article] [PubMed] [Google Scholar]
  5. Leyva-Cobián F., Clemente J. Phenotypic characterization and functional activity of human milk macrophages. Immunol Lett. 1984;8(5):249–256. doi: 10.1016/0165-2478(84)90004-x. [DOI] [PubMed] [Google Scholar]
  6. Martinez G. A., Nalezienski J. P. 1980 update: the recent trend in breast-feeding. Pediatrics. 1981 Feb;67(2):260–263. [PubMed] [Google Scholar]
  7. Mori M., Hayward A. R. Phenotype and function of human milk monocytes as antigen presenting cells. Clin Immunol Immunopathol. 1982 Apr;23(1):94–99. doi: 10.1016/0090-1229(82)90074-5. [DOI] [PubMed] [Google Scholar]
  8. Nunez G., Ball E. J., Stastny P. Accessory cell function of human endothelial cells. I. A subpopulation of Ia positive cells is required for antigen presentation. J Immunol. 1983 Aug;131(2):666–673. [PubMed] [Google Scholar]
  9. Pitt J., Barlow B., Heird W. C. Protection against experimental necrotizing enterocolitis by maternal milk. I. Role of milk leukocytes. Pediatr Res. 1977 Aug;11(8):906–909. doi: 10.1203/00006450-197708000-00011. [DOI] [PubMed] [Google Scholar]
  10. Seelig L. L., Jr, Billingham R. E. Concerning the natural transplantation of maternal lymphocytes via milk. Transplant Proc. 1981 Mar;13(1 Pt 2):1245–1249. [PubMed] [Google Scholar]
  11. Sheldrake R. F., Husband A. J. Intestinal uptake of intact maternal lymphocytes by neonatal rats and lambs. Res Vet Sci. 1985 Jul;39(1):10–15. [PubMed] [Google Scholar]
  12. Weiler I. J., Hickler W., Sprenger R. Demonstration that milk cells invade the suckling neonatal mouse. Am J Reprod Immunol. 1983 Sep;4(2):95–98. doi: 10.1111/j.1600-0897.1983.tb00261.x. [DOI] [PubMed] [Google Scholar]
  13. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]
  14. Yamashita U., Shevach E. M. The expression of Ia antigens on immunocompetent cells in the guinea pig. II. Ia antigens on macrophages. J Immunol. 1977 Nov;119(5):1584–1588. [PubMed] [Google Scholar]

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