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. 1988 Jun;64(2):261–266.

Non-specific regulatory mechanism of contact sensitivity: the requirement of intermediate cells for non-specific suppressor factor (NSF) activity.

Y Nakano 1
PMCID: PMC1384952  PMID: 2968951

Abstract

Non-specific suppressor factor (NSF), which inhibits passive transfer of contact sensitivity (CS), is produced spontaneously from macrophage-like suppressor cells which were induced by intravenous (i.v.) administration of oxazolone (Ox)-conjugated spleen cells. NSF is absorbed with normal spleen cells, and NSF-treated spleen cells acquire the ability to suppress the transfer of the effector cell function of CS non-specifically. In the present study, the events involved in the suppression by NSF were investigated. The involvement of intermediate cells between NSF and effector T cells in the suppression by NSF was suggested by the following observations: (i) NSF was absorbed with plastic-adherent and cyclophosphamide (CY)-sensitive non-T cells present in normal spleen cells; (ii) deletion of plastic adherent and CY-sensitive cells but not of adult thymectomy (ATx)-sensitive cells from the effector cell population, rendered the effector cells resistant to the suppressor activity of NSF; (iii) reconstitution of CY-pretreated effector cell population with Thy-1-negative spleen cells restored the ability of NSF to suppress CY-pretreated effector cells function. On the contrary, reconstitution with Ia-negative spleen cells did not restore the ability of NSF to suppress CY-pretreated effector cells function. Thus, NSF may not suppress directly the effector T-cell function, but intermediate cells, which are possibly macrophage-like cells, may exert a suppressive role after absorbing NSF. Species specificity was observed between the interaction of NSF and intermediate cells. The possible role of the intermediate cells in the suppression circuit of CS by NSF is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almawi W. Y., Pope B. L. Induction of suppression by a murine nonspecific suppressor-inducer cell line (M1-A5). II. The role of prostaglandins. J Immunol. 1986 Mar 15;136(6):1982–1987. [PubMed] [Google Scholar]
  2. Asherson G. L., Zembala M. The role of the T acceptor cell in suppressor systems. Antigen-specific T suppressor factor acts via a T acceptor cell; this releases a nonspecific inhibitor of the transfer of contact sensitivity when exposed to antigen in the context of I-J. Ann N Y Acad Sci. 1982;392:71–89. doi: 10.1111/j.1749-6632.1982.tb36099.x. [DOI] [PubMed] [Google Scholar]
  3. Moser G., Cheng G., Abbas A. K. Accessory cells in immune suppression. III. Evidence for two distinct accessory cell-dependent mechanisms of T lymphocyte-mediated suppression. J Immunol. 1986 Nov 15;137(10):3074–3079. [PubMed] [Google Scholar]
  4. Nakano Y. Antigenic competition in the induction of contact sensitivity in mice. Immunology. 1977 Aug;33(2):167–178. [PMC free article] [PubMed] [Google Scholar]
  5. Nakano Y., Nakano K. Different cellular requirements for inducing contact sensitivity and non-specific unresponsiveness with hapten-conjugated lymphoid cells. Immunology. 1985 Feb;54(2):307–316. [PMC free article] [PubMed] [Google Scholar]
  6. Nakano Y., Nakano K. Non-specific regulatory mechanism of contact sensitivity: induction of macrophage-like suppressor cells and their factors with hapten-conjugated lymphoid cells. Immunology. 1985 Feb;54(2):297–305. [PMC free article] [PubMed] [Google Scholar]
  7. Ptak W., Zembala M., Hanczakowski-Rewicka M., Asherson G. L. Nonspecific macrophage suppressor factor: its role in the inhibition of contact sensitivity to picryl chloride by specific T suppressor factor. Eur J Immunol. 1978 Sep;8(9):645–649. doi: 10.1002/eji.1830080908. [DOI] [PubMed] [Google Scholar]
  8. Sy M. S., Miller S. D., Moorhead J. W., Claman H. N. Active suppression of 1-fluoro-2,4-dinitrobenzene-immune T cells. Requirement of an auxiliary T cell induced by antigen. J Exp Med. 1979 May 1;149(5):1197–1207. doi: 10.1084/jem.149.5.1197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sy M. S., Nisonoff A., Germain R. N., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. VIII. Suppression of idiotype-negative, p-azobenzenearsonate-specific T cells results from the interaction of an anti-idiotypic second-order T suppressor cell with a cross-reactive-idiotype-positive, p-azobenzenearsonate-primed T cell target. J Exp Med. 1981 Jun 1;153(6):1415–1425. doi: 10.1084/jem.153.6.1415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Webb D. R., Nowowiejski I. Control of suppressor cell activation via endogenous prostaglandin synthesis: the role of T cells and macrophages. Cell Immunol. 1981 Sep 15;63(2):321–328. doi: 10.1016/0008-8749(81)90011-3. [DOI] [PubMed] [Google Scholar]

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