Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1988 Jun;64(2):337–340.

Biochemical identification and characterization of the G antigen in the human blood group Rh.

J Lirochon 1, C Doinel 1, P Rouger 1, D Goossens 1, C Salmon 1
PMCID: PMC1384965  PMID: 3134298

Abstract

The human blood group Rh comprises erythrocyte membrane antigens which include the G antigen. In order to carry out this study, the G antigen was specifically immunofixed with a human monoclonal anti-Rh(G) antibody. After extraction and purification of the antigenic complex, a migration in SDS-PAGE showed the G antigen to be associated with a polypeptide of 28,000 MW.

Full text

PDF
337

Images in this article

338Figure 1
on p.338
338Figure 2
on p.338
338Figure 3
on p.338

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloy C., Blanchard D., Lambin P., Goossens D., Rouger P., Salmon C., Cartron J. P. Human monoclonal antibody against Rh(D) antigen: partial characterization of the Rh(D) polypeptide from human erythrocytes. Blood. 1987 May;69(5):1491–1497. [PubMed] [Google Scholar]
  2. DODGE J. T., MITCHELL C., HANAHAN D. J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963 Jan;100:119–130. doi: 10.1016/0003-9861(63)90042-0. [DOI] [PubMed] [Google Scholar]
  3. Gahmberg C. G. Molecular characterization of the human red cell Rho(D) antigen. EMBO J. 1983;2(2):223–227. doi: 10.1002/j.1460-2075.1983.tb01409.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goossens D., Champomier F., Rouger P., Salmon C. Human monoclonal antibodies against blood group antigens. Preparation of a series of stable EBV immortalized B clones producing high levels of antibody of different isotypes and specificities. J Immunol Methods. 1987 Aug 3;101(2):193–200. doi: 10.1016/0022-1759(87)90150-5. [DOI] [PubMed] [Google Scholar]
  5. Krystal G., Graham A. F. A sensitive method for estimating the carbohydrate content of glycoproteins. Anal Biochem. 1976 Feb;70(2):336–345. doi: 10.1016/0003-2697(76)90454-1. [DOI] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Lirochon J., Salmon C., Rouger P., Doinel C. Radioactive labeling techniques for the identification of G antigen in the human Rh blood group system. Biochimie. 1987 Nov-Dec;69(11-12):1191–1197. doi: 10.1016/0300-9084(87)90146-5. [DOI] [PubMed] [Google Scholar]
  8. Steck T. L., Kant J. A. Preparation of impermeable ghosts and inside-out vesicles from human erythrocyte membranes. Methods Enzymol. 1974;31:172–180. doi: 10.1016/0076-6879(74)31019-1. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES