Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1988 Sep;65(1):37–42.

Stimulation of liposome-induced humoral immune responses by non-ionic block polymer surfactants in Xid mice.

G J Zigterman 1, M Jansze 1, H Snippe 1, J M Willers 1
PMCID: PMC1385016  PMID: 3053425

Abstract

Non-ionic block polymers (NBPs) have proved to be potent adjuvants for the humoral immune response against liposomes haptenated with tripeptide-enlarged dinitrophenyl groups (hapten J). Since both reversed triblocks and normal octablocks displayed adjuvant activity, reversed octablocks, in which structural properties of both groups are combined, were also tested for their adjuvant activity. The latter compounds displayed very strong adjuvant activity for J-haptenated liposomes, not only in normal BALB/c but also in (CBA/N x BALB/c)F1 progeny. To test the applicability of NBPs as adjuvants in semi-synthetic vaccines, the capacity of NBPs to stimulate the immune response against liposomes haptenated with Streptococcus pneumoniae type 3 capsular polysaccharide-derived oligosaccharides was analysed. In these studies, again NBPs proved potent adjuvants, stimulating antibody production to a large extent. In male (CBA/N x BALB/c)F1 mice, which carry a X-chromosome-linked immunodeficiency (Xid), antibody levels were stimulated to the largest extent by a normal octablock. Stimulation of antibody titres, however, did not result in increased protection in these Xid mice.

Full text

PDF
37

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C., Byars N. E. An adjuvant formulation that selectively elicits the formation of antibodies of protective isotypes and of cell-mediated immunity. J Immunol Methods. 1986 Dec 24;95(2):157–168. doi: 10.1016/0022-1759(86)90402-3. [DOI] [PubMed] [Google Scholar]
  2. Austrian R. A reassessment of pneumococcal vaccine. N Engl J Med. 1984 Mar 8;310(10):651–653. doi: 10.1056/NEJM198403083101010. [DOI] [PubMed] [Google Scholar]
  3. Baker P. J., Stashak P. W., Prescott B. Use of erythrocytes sensitized with purified pneumococcal polysaccharides for the assay of antibody and antibody-producing cells. Appl Microbiol. 1969 Mar;17(3):422–426. doi: 10.1128/am.17.3.422-426.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barrett D. J., Lee C. G., Ammann A. J., Ayoub E. M. IgG and IgM pneumococcal polysaccharide antibody responses in infants. Pediatr Res. 1984 Nov;18(11):1067–1071. doi: 10.1203/00006450-198411000-00001. [DOI] [PubMed] [Google Scholar]
  5. Douglas R. M., Miles H. B. Vaccination against Streptococcus pneumoniae in childhood: lack of demonstrable benefit in young Australian children. J Infect Dis. 1984 Jun;149(6):861–869. doi: 10.1093/infdis/149.6.861. [DOI] [PubMed] [Google Scholar]
  6. Granoff D. M., Cates K. L. Haemophilus influenzae type b polysaccharide vaccines. J Pediatr. 1985 Sep;107(3):330–336. doi: 10.1016/s0022-3476(85)80502-3. [DOI] [PubMed] [Google Scholar]
  7. Guckian J. C., Christensen G. D., Fine D. P. The role of opsonins in recovery from experimental pneumococcal pneumonia. J Infect Dis. 1980 Aug;142(2):175–190. doi: 10.1093/infdis/142.2.175. [DOI] [PubMed] [Google Scholar]
  8. Hilgers L. A., Snippe H., Jansze M., Willers J. M. Immunomodulating properties of two synthetic adjuvants: dependence upon type of antigen, dose, and time of administration. Cell Immunol. 1984 Jul;86(2):393–401. doi: 10.1016/0008-8749(84)90394-0. [DOI] [PubMed] [Google Scholar]
  9. Hunter R. L., Bennett B. The adjuvant activity of nonionic block polymer surfactants. II. Antibody formation and inflammation related to the structure of triblock and octablock copolymers. J Immunol. 1984 Dec;133(6):3167–3175. [PubMed] [Google Scholar]
  10. Inman J. K., Merchant B., Claflin L., Tacey S. E. Coupling of large haptens to proteins and cell surfaces: preparation of stable, optimally sensitized erythrocytes for hapten-specific, hemolytic plaque assays. Immunochemistry. 1973 Mar;10(3):165–174. doi: 10.1016/0019-2791(73)90005-0. [DOI] [PubMed] [Google Scholar]
  11. Kinsky S. C. Effects of lipid A on the immunologic properties of liposomal model membranes. Rev Infect Dis. 1984 Jul-Aug;6(4):558–562. doi: 10.1093/clinids/6.4.558. [DOI] [PubMed] [Google Scholar]
  12. Lee C. J., Takaoka Y., Saito T. Maternal immunization and the immune response of neonates to pneumococcal polysaccharides. Rev Infect Dis. 1987 May-Jun;9(3):494–510. doi: 10.1093/clinids/9.3.494. [DOI] [PubMed] [Google Scholar]
  13. Merchant B., Inman J. K. Hapten-specific hemolytic plaque assays usually fail to detect most of the diversity in the anti-hapten response. J Exp Med. 1977 Feb 1;145(2):372–389. doi: 10.1084/jem.145.2.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rijkers G. T., Dollekamp E. G., Zegers B. J. The in vitro B-cell response to pneumococcal polysaccharides in adults and neonates. Scand J Immunol. 1987 May;25(5):447–452. doi: 10.1111/j.1365-3083.1987.tb02215.x. [DOI] [PubMed] [Google Scholar]
  15. Snippe H., De Reuver M. J., Strickland F., Willers J. M., Hunter R. L. Adjuvant effect of nonionic block polymer surfactants in humoral and cellular immunity. Int Arch Allergy Appl Immunol. 1981;65(4):390–398. doi: 10.1159/000232780. [DOI] [PubMed] [Google Scholar]
  16. Snippe H., van Dam J. E., van Houte A. J., Willers J. M., Kamerling J. P., Vliegenthart J. F. Preparation of a semisynthetic vaccine to Streptococcus pneumoniae type 3. Infect Immun. 1983 Nov;42(2):842–844. doi: 10.1128/iai.42.2.842-844.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Snippe H., van Houte A. J., Lizzio E. F., Willers J. M., Merchant B. Characterization of immunogenic properties of haptenated liposomal model membranes in mice. VI. Response in B-cell-defective CBA/N mice. Immunology. 1982 Mar;45(3):545–551. [PMC free article] [PubMed] [Google Scholar]
  18. Snippe H., van Houte A. J., van Dam J. E., De Reuver M. J., Jansze M., Willers J. M. Immunogenic properties in mice of hexasaccharide from the capsular polysaccharide of Streptococcus pneumoniae type 3. Infect Immun. 1983 Jun;40(3):856–861. doi: 10.1128/iai.40.3.856-861.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Svenson S. B., Lindberg A. A. Coupling of acid labile Salmonella specific oligosaccharides to macromolecular carriers. J Immunol Methods. 1979;25(4):323–335. doi: 10.1016/0022-1759(79)90025-5. [DOI] [PubMed] [Google Scholar]
  20. Tadakuma T., Yasuda T., Tamauchi H., Saito K., Tsumita T., Kinsky S. C. Effect of lipid A incorporation on characterization of liposomal model membranes as thymus-independent type 1 or type 2 immunogens. J Immunol. 1982 Jan;128(1):206–210. [PubMed] [Google Scholar]
  21. Wicker L. S., Scher I. X-linked immune deficiency (xid) of CBA/N mice. Curr Top Microbiol Immunol. 1986;124:87–101. doi: 10.1007/978-3-642-70986-9_6. [DOI] [PubMed] [Google Scholar]
  22. Zigterman G. J., Jansze M., Snippe H., Willers J. M. Immunomodulating properties of substances to be used in combination with liposomes. Int Arch Allergy Appl Immunol. 1986;81(3):245–252. doi: 10.1159/000234142. [DOI] [PubMed] [Google Scholar]
  23. Zigterman G. J., Snippe H., Jansze M., Willers J. M. Adjuvant effects of nonionic block polymer surfactants on liposome-induced humoral immune response. J Immunol. 1987 Jan 1;138(1):220–225. [PubMed] [Google Scholar]
  24. Zigterman J. W., van Dam J. E., Snippe H., Rotteveel F. T., Jansze M., Willers J. M., Kamerling J. P., Vliegenthart J. F. Immunogenic properties of octasaccharide-protein conjugates derived from Klebsiella serotype 11 capsular polysaccharide. Infect Immun. 1985 Feb;47(2):421–428. doi: 10.1128/iai.47.2.421-428.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES