Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1989 Feb;66(2):233–237.

Characterization of the spontaneous autoimmune (anti-erythrocyte) response in NZB mice using a pathogenic monoclonal autoantibody and its anti-idiotype.

M J Caulfield 1, D Stanko 1, C Calkins 1
PMCID: PMC1385093  PMID: 2925223

Abstract

A monoclonal anti-mouse RBC antibody (G-8) has been prepared that appears to represent a pathogenic autoantibody related to those that arise spontaneously in aging NZB mice and which cause autoimmune haemolytic disease (AIHD). When G-8-producing hybridoma cells were grown as tumours in BALB/c mice, the mice developed AIHD characterized by a decrease in the number of erythrocytes (haematocrit) and the development of Coombs-positivity. An anti-idiotypic antibody (E8) was prepared against G-8 and was found to recognize an idiotypic determinant present on most autoantibody-forming cells derived from old (Coombs-positive) NZB mice. The results suggest that the IgM autoantibody response of NZB mice to self-erythrocytes is restricted to a limited number of clones, most of which express the G-8 idiotype.

Full text

PDF
233

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrzejewski C., Jr, Rauch J., Lafer E., Stollar B. D., Schwartz R. S. Antigen-binding diversity and idiotypic cross-reactions among hybridoma autoantibodies to DNA. J Immunol. 1981 Jan;126(1):226–231. [PubMed] [Google Scholar]
  2. Cianfriglia M., Armellini D., Massone A., Mariani M. Simple immunization protocol for high frequency production of soluble antigen-specific hybridomas. Hybridoma. 1983;2(4):451–457. doi: 10.1089/hyb.1983.2.451. [DOI] [PubMed] [Google Scholar]
  3. Cooke L. A., Staines N. A., Morgan A., Moorhouse C., Harris G. Haemolytic disease in mice induced by transplantation of hybridoma cells secreting monoclonal anti-erythrocyte autoantibodies. Immunology. 1982 Nov;47(3):569–572. [PMC free article] [PubMed] [Google Scholar]
  4. Deheer D. H., Edginton T. S. Cellular events associated with the immunogenesis of anti-erythrocyte autoantibody responses of NZB mice. Transplant Rev. 1976;31:116–155. doi: 10.1111/j.1600-065x.1976.tb01453.x. [DOI] [PubMed] [Google Scholar]
  5. Dwyer D. S., Bradley R. J., Urquhart C. K., Kearney J. F. Naturally occurring anti-idiotypic antibodies in myasthenia gravis patients. Nature. 1983 Feb 17;301(5901):611–614. doi: 10.1038/301611a0. [DOI] [PubMed] [Google Scholar]
  6. Evans M. M., Williamson W. G., Irvine W. J. The appearance of immunological competence at an early age in New Zealand black mice. Clin Exp Immunol. 1968 Jun;3(5):375–383. [PMC free article] [PubMed] [Google Scholar]
  7. Goding J. W. Antibody production by hybridomas. J Immunol Methods. 1980;39(4):285–308. doi: 10.1016/0022-1759(80)90230-6. [DOI] [PubMed] [Google Scholar]
  8. Goding J. W. Use of staphylococcal protein A as an immunological reagent. J Immunol Methods. 1978;20:241–253. doi: 10.1016/0022-1759(78)90259-4. [DOI] [PubMed] [Google Scholar]
  9. Hahn B. H., Ebling F., Freeman S., Clevinger B., Davie J. Production of monoclonal murine antibodies to DNA by somatic cell hybrids. Arthritis Rheum. 1980 Aug;23(8):942–945. doi: 10.1002/art.1780230811. [DOI] [PubMed] [Google Scholar]
  10. Kapp J. A., Pierce C. W., De la Croix F., Benacerraf B. Immunosuppressive factor(s) extracted from lymphoid cells of nonresponder mice primed with L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT). J Immunol. 1976 Feb;116(2):305–309. [PubMed] [Google Scholar]
  11. Kearney J. F., Radbruch A., Liesegang B., Rajewsky K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol. 1979 Oct;123(4):1548–1550. [PubMed] [Google Scholar]
  12. LONG G., HOLMES M. C., BURNET F. M. AUTOANTIBODIES PRODUCED AGAINST MOUSE ERYTHROCYTES IN NZB MICE. Aust J Exp Biol Med Sci. 1963 Aug;41:315–322. doi: 10.1038/icb.1963.31. [DOI] [PubMed] [Google Scholar]
  13. Lafer E. M., Rauch J., Andrzejewski C., Jr, Mudd D., Furie B., Furie B., Schwartz R. S., Stollar B. D. Polyspecific monoclonal lupus autoantibodies reactive with both polynucleotides and phospholipids. J Exp Med. 1981 Apr 1;153(4):897–909. doi: 10.1084/jem.153.4.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miller R. D., Calkins C. E. Active role of T cells in promoting an in vitro autoantibody response to self erythrocytes in NZB mice. Immunology. 1988 Apr;63(4):625–630. [PMC free article] [PubMed] [Google Scholar]
  15. Moore J. S., Calkins C. E. In vitro regulation of the pathogenic autoantibody response of New Zealand black mice. I. Loss with age of suppressive activity in T cell populations. J Immunol. 1985 Jun;134(6):3838–3844. [PubMed] [Google Scholar]
  16. Moore J., Calkins C. Formation of antigen specific foci as a complement independent assay for individual antibody-secreting cells. J Immunol Methods. 1983 Oct 28;63(3):377–384. doi: 10.1016/s0022-1759(83)80011-8. [DOI] [PubMed] [Google Scholar]
  17. Naparstek Y., André-Schwartz J., Manser T., Wysocki L. J., Breitman L., Stollar B. D., Gefter M., Schwartz R. S. A single germline VH gene segment of normal A/J mice encodes autoantibodies characteristic of systemic lupus erythematosus. J Exp Med. 1986 Aug 1;164(2):614–626. doi: 10.1084/jem.164.2.614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pages J. M., Bussard A. E. Establishment and characterization of a permanent murine hybridoma secreting monoclonal autoantibodies. Cell Immunol. 1978 Nov;41(1):188–194. doi: 10.1016/s0008-8749(78)80038-0. [DOI] [PubMed] [Google Scholar]
  19. RUBIN H. Antibody elution from red blood cells. J Clin Pathol. 1963 Jan;16:70–73. doi: 10.1136/jcp.16.1.70. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Roitt I. M., Male D. K., Cooke A., Lydyard P. M. Idiotypes and autoimmunity. Springer Semin Immunopathol. 1983;6(1):51–66. doi: 10.1007/BF01857366. [DOI] [PubMed] [Google Scholar]
  21. Shulman M., Wilde C. D., Köhler G. A better cell line for making hybridomas secreting specific antibodies. Nature. 1978 Nov 16;276(5685):269–270. doi: 10.1038/276269a0. [DOI] [PubMed] [Google Scholar]
  22. Staples P. J., Talal N. Relative inability to induce tolerance in adult NZB and NZB-NZW F1 mice. J Exp Med. 1969 Jan 1;129(1):123–139. doi: 10.1084/jem.129.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES