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. 1988 May;64(1):97–99.

Proliferation of thymocytes in relation to T-cell receptor beta-chain expression.

I G Parkin 1, J J Owen 1, E J Jenkinson 1
PMCID: PMC1385192  PMID: 3290094

Abstract

During proliferation and differentiation of maturing thymocytes, T-cell receptor beta-chain products are first expressed in the cytoplasm. Only subsequently are they expressed on the cell surface, presumably as part of the alpha beta/CD3 receptor complex. This study uses double immunofluorescence labelling to identify these cytoplasmic and surface phases separately in relationship to cell-cycle parameters. The use of a mitotic arrest agent and tritiated thymidine autoradiography both show that cells with cytoplasmic beta-chains are in cell cycle, whereas cells with surface beta-chains are cycling slowly, if at all.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aherne W., Camplejohn R. S. On correcting the error due to metaphase degeneration in stathmokinetic studies. Exp Cell Res. 1972 Oct;74(2):496–502. doi: 10.1016/0014-4827(72)90406-5. [DOI] [PubMed] [Google Scholar]
  2. Jenkinson E. J., Kingston R., Owen J. J. Importance of IL-2 receptors in intra-thymic generation of cells expressing T-cell receptors. Nature. 1987 Sep 10;329(6135):160–162. doi: 10.1038/329160a0. [DOI] [PubMed] [Google Scholar]
  3. Osmond D. G., Owen J. J. Pre-B cells in bone marrow: size distribution profile, proliferative capacity and peanut agglutinin binding of cytoplasmic mu chain-bearing cell populations in normal and regenerating bone marrow. Immunology. 1984 Feb;51(2):333–342. [PMC free article] [PubMed] [Google Scholar]
  4. Snodgrass H. R., Kisielow P., Kiefer M., Steinmetz M., von Boehmer H. Ontogeny of the T-cell antigen receptor within the thymus. Nature. 1985 Feb 14;313(6003):592–595. doi: 10.1038/313592a0. [DOI] [PubMed] [Google Scholar]
  5. Staerz U. D., Rammensee H. G., Benedetto J. D., Bevan M. J. Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor. J Immunol. 1985 Jun;134(6):3994–4000. [PubMed] [Google Scholar]

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