Abstract
During proliferation and differentiation of maturing thymocytes, T-cell receptor beta-chain products are first expressed in the cytoplasm. Only subsequently are they expressed on the cell surface, presumably as part of the alpha beta/CD3 receptor complex. This study uses double immunofluorescence labelling to identify these cytoplasmic and surface phases separately in relationship to cell-cycle parameters. The use of a mitotic arrest agent and tritiated thymidine autoradiography both show that cells with cytoplasmic beta-chains are in cell cycle, whereas cells with surface beta-chains are cycling slowly, if at all.
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- Aherne W., Camplejohn R. S. On correcting the error due to metaphase degeneration in stathmokinetic studies. Exp Cell Res. 1972 Oct;74(2):496–502. doi: 10.1016/0014-4827(72)90406-5. [DOI] [PubMed] [Google Scholar]
- Jenkinson E. J., Kingston R., Owen J. J. Importance of IL-2 receptors in intra-thymic generation of cells expressing T-cell receptors. Nature. 1987 Sep 10;329(6135):160–162. doi: 10.1038/329160a0. [DOI] [PubMed] [Google Scholar]
- Osmond D. G., Owen J. J. Pre-B cells in bone marrow: size distribution profile, proliferative capacity and peanut agglutinin binding of cytoplasmic mu chain-bearing cell populations in normal and regenerating bone marrow. Immunology. 1984 Feb;51(2):333–342. [PMC free article] [PubMed] [Google Scholar]
- Snodgrass H. R., Kisielow P., Kiefer M., Steinmetz M., von Boehmer H. Ontogeny of the T-cell antigen receptor within the thymus. Nature. 1985 Feb 14;313(6003):592–595. doi: 10.1038/313592a0. [DOI] [PubMed] [Google Scholar]
- Staerz U. D., Rammensee H. G., Benedetto J. D., Bevan M. J. Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor. J Immunol. 1985 Jun;134(6):3994–4000. [PubMed] [Google Scholar]