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. 1988 May;64(1):113–119.

The role of autoreactive T-cell hybridomas from autoimmune model mice.

S Yanoma 1, I Aoki 1, N Ishii 1, K Tani 1, C S David 1, K Okuda 1
PMCID: PMC1385195  PMID: 3260213

Abstract

Autoreactive IL-2-producing T-cell hybridomas were established from New Zealand Black and White (B/W) F1 and MRL/1 mice. In B/WF1 mice the frequency of IL-2-producing hybridomas increased with age. It is necessary for the cells to recognize autologous MHC molecules in order to release IL-2. Inoculation of hybridoma cells into several mouse strains via the footpad produced significant swelling responses in an H-2-restricted manner. Finally, several autoimmune abnormalities were induced in naive H-2-compatible mice by i.v. inoculation of certain hybridoma cells. These results demonstrate that self-MHC molecule recognition by T cells plays an important role in the development of autoimmunity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altman A., Theofilopoulos A. N., Weiner R., Katz D. H., Dixon F. J. Analysis of T cell function in autoimmune murine strains. Defects in production and responsiveness to interleukin 2. J Exp Med. 1981 Sep 1;154(3):791–808. doi: 10.1084/jem.154.3.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bensussan A., Meuer S. C., Schlossman S. F., Reinherz E. L. Delineation of an immunoregulatory amplifier population recognizing autologous Ia molecules. Analysis with human T cell clones. J Exp Med. 1984 Feb 1;159(2):559–576. doi: 10.1084/jem.159.2.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bottazzo G. F., Pujol-Borrell R., Hanafusa T., Feldmann M. Role of aberrant HLA-DR expression and antigen presentation in induction of endocrine autoimmunity. Lancet. 1983 Nov 12;2(8359):1115–1119. doi: 10.1016/s0140-6736(83)90629-3. [DOI] [PubMed] [Google Scholar]
  4. Dauphinée M. J., Kipper S. B., Wofsy D., Talal N. Interleukin 2 deficiency is a common feature of autoimmune mice. J Immunol. 1981 Dec;127(6):2483–2487. [PubMed] [Google Scholar]
  5. Dialynas D. P., Quan Z. S., Wall K. A., Pierres A., Quintáns J., Loken M. R., Pierres M., Fitch F. W. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445–2451. [PubMed] [Google Scholar]
  6. Gleichmann E., Van Elven E. H., Van der Veen J. P. A systemic lupus erythematosus (SLE)-like disease in mice induced by abnormal T-B cell cooperation. Preferential formation of autoantibodies characteristic of SLE. Eur J Immunol. 1982 Feb;12(2):152–159. doi: 10.1002/eji.1830120210. [DOI] [PubMed] [Google Scholar]
  7. Glimcher L. H., Sharrow S. O., Paul W. E. Serologic and functional characterization of a panel of antigen-presenting cell lines expressing mutant I-A class II molecules. J Exp Med. 1983 Nov 1;158(5):1573–1588. doi: 10.1084/jem.158.5.1573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hausman P. B., Stobo J. D. Specificity and function of a human autologous reactive T cell. J Exp Med. 1979 Jun 1;149(6):1537–1542. doi: 10.1084/jem.149.6.1537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kappler J. W., Skidmore B., White J., Marrack P. Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition. J Exp Med. 1981 May 1;153(5):1198–1214. doi: 10.1084/jem.153.5.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leung D. Y., Parkman R., Feller J., Wood N., Geha R. S. Cell-mediated cytotoxicity against skin fibroblasts in atopic dermatitis. J Immunol. 1982 Apr;128(4):1736–1741. [PubMed] [Google Scholar]
  11. Linker-Israeli M., Bakke A. C., Kitridou R. C., Gendler S., Gillis S., Horwitz D. A. Defective production of interleukin 1 and interleukin 2 in patients with systemic lupus erythematosus (SLE). J Immunol. 1983 Jun;130(6):2651–2655. [PubMed] [Google Scholar]
  12. Minami M., Okuda K., Sunday M. E., Dorf M. E. H-2K-, H-21- and H-2D-restricted hybridoma contact sensitivity effector cells. Nature. 1982 May 20;297(5863):231–233. doi: 10.1038/297231a0. [DOI] [PubMed] [Google Scholar]
  13. Nussenzweig M. C., Steinman R. M. Contribution of dendritic cells to stimulation of the murine syngeneic mixed leukocyte reaction. J Exp Med. 1980 May 1;151(5):1196–1212. doi: 10.1084/jem.151.5.1196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oi V. T., Jones P. P., Goding J. W., Herzenberg L. A., Herzenberg L. A. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978;81:115–120. doi: 10.1007/978-3-642-67448-8_18. [DOI] [PubMed] [Google Scholar]
  15. Okuda K., Minami M., Ju S. T., Dorf M. E. Functional association of idiotypic and I-J determinants on the antigen receptor of suppressor T cells. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4557–4561. doi: 10.1073/pnas.78.7.4557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Opelz G., Kiuchi M., Takasugi M., Terasaki P. I. Autologous stimulation of human lymphocyte subpopulation. J Exp Med. 1975 Nov 1;142(5):1327–1333. doi: 10.1084/jem.142.5.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reinitz E., Hubbard D., Grayzel A. I. Central nervous system systemic lupus erythematosus versus central nervous system infection: low cerebral spinal fluid glucose and pleocytosis in a patient with a prolonged course. Arthritis Rheum. 1982 May;25(5):583–587. doi: 10.1002/art.1780250515. [DOI] [PubMed] [Google Scholar]
  18. Saito K., Tamura A., Narimatsu H., Tadakuma T., Nagashima M. Cloned auto-Ia-reactive T cells elicit lichen planus-like lesion in the skin of syngeneic mice. J Immunol. 1986 Oct 15;137(8):2485–2495. [PubMed] [Google Scholar]
  19. Sakane T., Steinberg A. D., Green I. Failure of autologous mixed lymphocyte reactions between T and non-T cells in patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3464–3468. doi: 10.1073/pnas.75.7.3464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Smith J. B., Pasternak R. D. Syngeneic mixed lymphocyte reaction in mice: strain distribution, kinetics, participating cells, and absence in NZB mice. J Immunol. 1978 Nov;121(5):1889–1892. [PubMed] [Google Scholar]
  21. Takada S., Ueda Y., Suzuki N., Murakawa Y., Hoshino T., Green I., Steinberg A. D., Horwitz D. A., Sakane T. Abnormalities in autologous mixed lymphocyte reaction-activated immunologic processes in systemic lupus erythematosus and their possible correction by interleukin 2. Eur J Immunol. 1985 Mar;15(3):262–267. doi: 10.1002/eji.1830150310. [DOI] [PubMed] [Google Scholar]
  22. Yamashita U., Shevach E. M. The syngeneic mixed leukocyte reaction: the genetic requirements for the recognition of self resemble the requirements for the recognition of antigen in association with self. J Immunol. 1980 Apr;124(4):1773–1778. [PubMed] [Google Scholar]

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