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. 1988 May;64(1):175–180.

The therapeutic action of monoclonal antibodies against a surface glycoprotein of Giardia muris.

W G Butscher 1, G M Faubert 1
PMCID: PMC1385204  PMID: 2454885

Abstract

Twenty-three monoclonal antibodies (mAbs) against Giardia muris were obtained in two fusions using spleen cells of immunized mice. Similarly, the fusion of mesenteric lymph node (MLN) cells of an infected mouse yielded 15 mAbs. Three mAbs arising from spleen cells and two from MLN (all five were IgM) were able to kill trophozoites in the presence of guinea-pig complement (GPC). They can agglutinate trophozoites and also impair the movement of the flagella in the absence of GPC. I.p. treatment of mice with mAb 45C on Days -1, 1, 3 and 5 of infection significantly reduced the intestinal parasite burden. Indirect immunofluorescence assays on live and fixed trophozoites revealed that the cytotoxic mAbs were directed against antigens located on the periphery of the body, the sucking disc, the flagella and the ventro-lateral flange. In Western blots, our mAbs recognized major 36,200 and 30,300 MW glycoproteins located on the surface of the parasite.

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Selected References

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  1. Anders R. F., Roberts-Thomson I. C., Mitchell G. F. Giardiasis in mice: analysis of humoral and cellular immune responses to Giardia muris. Parasite Immunol. 1982 Jan;4(1):47–57. doi: 10.1111/j.1365-3024.1982.tb00419.x. [DOI] [PubMed] [Google Scholar]
  2. Belosevic M., Faubert G. M. Temporal study of acquired resistance in infections of mice with Giardia muris. Parasitology. 1983 Dec;87(Pt 3):517–524. doi: 10.1017/s0031182000083037. [DOI] [PubMed] [Google Scholar]
  3. Birkett C. R., Foster K. E., Johnson L., Gull K. Use of monoclonal antibodies to analyse the expression of a multi-tubulin family. FEBS Lett. 1985 Aug 5;187(2):211–218. doi: 10.1016/0014-5793(85)81244-8. [DOI] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. Clark J. T., Holberton D. V. Plasma membrane isolated from Giardia lamblia: identification of membrane proteins. Eur J Cell Biol. 1986 Dec;42(2):200–206. [PubMed] [Google Scholar]
  6. Crossley R., Holberton D. Assembly of 2.5 nm filaments from giardin, a protein associated with cytoskeletal microtubules in Giardia. J Cell Sci. 1985 Oct;78:205–231. doi: 10.1242/jcs.78.1.205. [DOI] [PubMed] [Google Scholar]
  7. Crossley R., Marshall J., Clark J. T., Holberton D. V. Immunocytochemical differentiation of microtubules in the cytoskeleton of Giardia lamblia using monoclonal antibodies to alpha-tubulin and polyclonal antibodies to associated low molecular weight proteins. J Cell Sci. 1986 Feb;80:233–252. doi: 10.1242/jcs.80.1.233. [DOI] [PubMed] [Google Scholar]
  8. Erlich J. H., Anders R. F., Roberts-Thomson I. C., Schrader J. W., Mitchell G. F. An examination of differences in serum antibody specificities and hypersensitivity reactions as contributing factors to chronic infection with the intestinal protozoan parasite, Giardia muris, in mice. Aust J Exp Biol Med Sci. 1983 Oct;61(Pt 5):599–615. doi: 10.1038/icb.1983.57. [DOI] [PubMed] [Google Scholar]
  9. Gillin F. D., Diamond L. S. Entamoeba histolytica and Giardia lamblia: effects of cysteine and oxygen tension on trophozoite attachment to glass and survival in culture media. Exp Parasitol. 1981 Aug;52(1):9–17. doi: 10.1016/0014-4894(81)90055-2. [DOI] [PubMed] [Google Scholar]
  10. Gillon J., Al Thamery D., Ferguson A. Features of small intestinal pathology (epithelial cell kinetics, intraepithelial lymphocytes, disaccharidases) in a primary Giardia muris infection. Gut. 1982 Jun;23(6):498–506. doi: 10.1136/gut.23.6.498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heyworth M. F. Antibody response to Giardia muris trophozoites in mouse intestine. Infect Immun. 1986 May;52(2):568–571. doi: 10.1128/iai.52.2.568-571.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hill D. R., Burge J. J., Pearson R. D. Susceptibility of Giardia lamblia trophozoites to the lethal effect of human serum. J Immunol. 1984 Apr;132(4):2046–2052. [PubMed] [Google Scholar]
  13. Holberton D. V. Attachment of Giardia-a hydrodynamic model based on flagellar activity. J Exp Biol. 1974 Feb;60(1):207–221. doi: 10.1242/jeb.60.1.207. [DOI] [PubMed] [Google Scholar]
  14. Holberton D. V., Ward A. P. Isolation of the cytoskeleton from Giardia. Tubulin and a low-molecular-weight protein associated with microribbon structures. J Cell Sci. 1981 Feb;47:139–166. doi: 10.1242/jcs.47.1.139. [DOI] [PubMed] [Google Scholar]
  15. Kaplan B. S., Uni S., Aikawa M., Mahmoud A. A. Effector mechanism of host resistance in murine giardiasis: specific IgG and IgA cell-mediated toxicity. J Immunol. 1985 Mar;134(3):1975–1981. [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Nash T. E., Aggarwal A. Cytotoxicity of monoclonal antibodies to a subset of Giardia isolates. J Immunol. 1986 Apr 1;136(7):2628–2632. [PubMed] [Google Scholar]
  18. Roberts-Thomson I. C., Stevens D. P., Mahmoud A. A., Warren K. S. Acquired resistance to infection in an animal model of giardiasis. J Immunol. 1976 Nov;117(5 PT2):2036–2037. [PubMed] [Google Scholar]
  19. Snider D. P., Gordon J., McDermott M. R., Underdown B. J. Chronic Giardia muris infection in anti-IgM-treated mice. I. Analysis of immunoglobulin and parasite-specific antibody in normal and immunoglobulin-deficient animals. J Immunol. 1985 Jun;134(6):4153–4162. [PubMed] [Google Scholar]
  20. Taylor G. D., Wenman W. M. Human immune response to Giardia lamblia infection. J Infect Dis. 1987 Jan;155(1):137–140. doi: 10.1093/infdis/155.1.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Underdown B. J., Roberts-Thomson I. C., Anders R. F., Mitchell G. F. Giardiasis in mice: studies on the characteristics of chronic infection in C3h/He mice. J Immunol. 1981 Feb;126(2):669–672. [PubMed] [Google Scholar]

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