Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1989 Mar;66(3):339–342.

Antigenic structure of the hexacosapeptide melittin: evidence for three determinants, one with a helical conformation.

R von Grünigen 1, C H Schneider 1
PMCID: PMC1385217  PMID: 2467877

Abstract

ELISA-based epitope analysis was performed using rabbit polyclonal antisera against melittin. Antigenic sites were found at the C-terminus, in the middle section and within the N-terminal helix. Antibodies against the helical segment could discriminate between two faces of the amphiphilic helix. The antigenic sites include the bulk of the melittin hexacosapeptide, which is synonymous with a very high epitope density.

Full text

PDF
339

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benjamin D. C., Berzofsky J. A., East I. J., Gurd F. R., Hannum C., Leach S. J., Margoliash E., Michael J. G., Miller A., Prager E. M. The antigenic structure of proteins: a reappraisal. Annu Rev Immunol. 1984;2:67–101. doi: 10.1146/annurev.iy.02.040184.000435. [DOI] [PubMed] [Google Scholar]
  2. Brown L. R., Lauterwein J., Wüthrich K. High-resolution 1H-NMR studies of self-aggregation of melittin in aqueous solution. Biochim Biophys Acta. 1980 Apr 25;622(2):231–244. doi: 10.1016/0005-2795(80)90034-3. [DOI] [PubMed] [Google Scholar]
  3. Drake A. F., Hider R. C. The structure of melittin in lipid bilayer membranes. Biochim Biophys Acta. 1979 Aug 7;555(2):371–373. doi: 10.1016/0005-2736(79)90178-0. [DOI] [PubMed] [Google Scholar]
  4. FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
  5. Horejsí V., Matousek V. Equilibrium in the protein-immobilized-ligand-soluble-ligand system: estimation of dissociation constants of protein-soluble-ligand complexes from binding-inhibition data. Mol Immunol. 1985 Feb;22(2):125–133. doi: 10.1016/s0161-5890(85)80006-7. [DOI] [PubMed] [Google Scholar]
  6. Kasper M. F., Schneider C. H., Rolli H., Angst B. D., de Weck A. L. Diagnostic reagents in drug allergy: immunochemical specificity in the 1,2-diphenyl-pyrazolidinedione series. Immunobiology. 1986 Oct;173(1):98–109. doi: 10.1016/S0171-2985(86)80093-6. [DOI] [PubMed] [Google Scholar]
  7. König W., Geiger R. Eine neue Methode zur Synthese von Peptiden: Aktivierung der Carboxylgruppe mit Dicyclohexycarbodiimid unter Zusatz von 1-Hydroxy-benzotriazolen. Chem Ber. 1970;103(3):788–798. doi: 10.1002/cber.19701030319. [DOI] [PubMed] [Google Scholar]
  8. Lauterwein J., Brown L. R., Wüthrich K. High-resolution 1H-NMR studies of monomeric melittin in aqueous solution. Biochim Biophys Acta. 1980 Apr 25;622(2):219–230. doi: 10.1016/0005-2795(80)90033-1. [DOI] [PubMed] [Google Scholar]
  9. Toniolo C., Bonora G. M., Lüscher I. F., Schneider C. H. Chain-length dependence for secondary structure formation of homo-oligopeptides from epsilon-tert.-butyloxycarbonyl-L-lysine with a lipophilic C-terminal group. Int J Pept Protein Res. 1984 Jan;23(1):47–54. doi: 10.1111/j.1399-3011.1984.tb02691.x. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES