Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1989 Jun;67(2):176–180.

Monoclonal antibodies to ovine SBU-T8 and SBU-T6 bind analogous molecules on bovine lymphocytes.

K L O'Reilly 1, G A Splitter 1
PMCID: PMC1385253  PMID: 2787776

Abstract

Monoclonal antibodies (mAb) to ovine T-lymphocyte molecules SBU-T8 (Maddox, Mackay & Brandon, 1985), the cytotoxic T lymphocyte, human CD8 equivalent, and SBU-T6 (Mackay et al., 1985), the immature T lymphocyte human CD1 equivalent, were reported to cross-react with bovine cells. We have confirmed this cross-reactivity and shown that the percentage of T lymphocytes in thymus, lymph node and peripheral blood is equivalent to that reported in sheep. In addition, we have shown that the molecular weight of the molecules identified by these antibodies (T8, 34,000-38,000; T6, 44,000 and 13,000) is similar to that reported in sheep and humans. The monoclonal antibody to SBU-T8 effectively removes bovine cytotoxic cells in the presence of complement and when used alone blocks killing of allogeneic concanavalin A (Con A) lymphoblasts by mixed lymphocyte-generated cytotoxic T lymphocytes. These results confirm that the anti-SBU-T8 antibody 38-65 binds BoCD8 and anti-SBU-T6 antibody 20-27 binds BoCD1 and that these reagents will be useful in the study of the bovine immune system.

Full text

PDF
176

Images in this article

178Figure 3
on p.178

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baldwin C. L., Machugh N. D., Ellis J. A., Naessens J., Newson J., Morrison W. I. Monoclonal antibodies which react with bovine T-lymphocyte antigens and induce blastogenesis: tissue distribution and functional characteristics of the target antigens. Immunology. 1988 Mar;63(3):439–446. [PMC free article] [PubMed] [Google Scholar]
  2. Baldwin C. L., Teale A. J., Naessens J. G., Goddeeris B. M., MacHugh N. D., Morrison W. I. Characterization of a subset of bovine T lymphocytes that express BoT4 by monoclonal antibodies and function: similarity to lymphocytes defined by human T4 and murine L3T4. J Immunol. 1986 Jun 15;136(12):4385–4391. [PubMed] [Google Scholar]
  3. Bekoff M., Kubo R., Grey H. M. Activation requirements for normal T cells: accessory cell-dependent and -independent stimulation by anti-receptor antibodies. J Immunol. 1986 Sep 1;137(5):1411–1419. [PubMed] [Google Scholar]
  4. Davis W. C., Ellis J. A., MacHugh N. D., Baldwin C. L. Bovine pan T-cell monoclonal antibodies reactive with a molecule similar to CD2. Immunology. 1988 Jan;63(1):165–167. [PMC free article] [PubMed] [Google Scholar]
  5. Davis W. C., Marusic S., Lewin H. A., Splitter G. A., Perryman L. E., McGuire T. C., Gorham J. R. The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species. Vet Immunol Immunopathol. 1987 Jul;15(4):337–376. doi: 10.1016/0165-2427(87)90005-5. [DOI] [PubMed] [Google Scholar]
  6. Ellis J. A., Baldwin C. L., MacHugh N. D., Bensaid A., Teale A. J., Goddeeris B. M., Morrison W. I. Characterization by a monoclonal antibody and functional analysis of a subset of bovine T lymphocytes that express BoT8, a molecule analogous to human CD8. Immunology. 1986 Jul;58(3):351–358. [PMC free article] [PubMed] [Google Scholar]
  7. Fan J., Ahmed A., Bonavida B. Studies on the induction and expression of T cell-mediated immunity. X. Inhibition by Lyt 2,3 antisera of cytotoxic T lymphocyte-mediated antigen-specific and -nonspecific cytotoxicity: evidence for the blocking of the binding between T lymphocytes and target cells and not the post-binding cytolytic steps. J Immunol. 1980 Dec;125(6):2444–2453. [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Ledbetter J. A., Evans R. L., Lipinski M., Cunningham-Rundles C., Good R. A., Herzenberg L. A. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981 Feb 1;153(2):310–323. doi: 10.1084/jem.153.2.310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MacDonald H. R., Glasebrook A. L., Cerottini J. C. Clonal heterogeneity in the functional requirement for Lyt-2/3 molecules on cytolytic T lymphocytes: analysis by antibody blocking and selective trypsinization. J Exp Med. 1982 Dec 1;156(6):1711–1722. doi: 10.1084/jem.156.6.1711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mackay C. R., Maddox J. F., Brandon M. R. Three distinct subpopulations of sheep T lymphocytes. Eur J Immunol. 1986 Jan;16(1):19–25. doi: 10.1002/eji.1830160105. [DOI] [PubMed] [Google Scholar]
  12. Mackay C. R., Maddox J. F., Gogolin-Ewens K. J., Brandon M. R. Characterization of two sheep lymphocyte differentiation antigens, SBU-T1 and SBU-T6. Immunology. 1985 Aug;55(4):729–737. [PMC free article] [PubMed] [Google Scholar]
  13. Maddox J. F., Mackay C. R., Brandon M. R. Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies. Immunology. 1985 Aug;55(4):739–748. [PMC free article] [PubMed] [Google Scholar]
  14. Mason D. W., Arthur R. P., Dallman M. J., Green J. R., Spickett G. P., Thomas M. L. Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies. Immunol Rev. 1983;74:57–82. doi: 10.1111/j.1600-065x.1983.tb01084.x. [DOI] [PubMed] [Google Scholar]
  15. Nielsen K. H., Henning M. D., Duncan J. R. Monoclonal antibodies in veterinary medicine. Biotechnol Genet Eng Rev. 1986;4:311–353. doi: 10.1080/02648725.1986.10647831. [DOI] [PubMed] [Google Scholar]
  16. Pescovitz M. D., Lunney J. K., Sachs D. H. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J Immunol. 1984 Jul;133(1):368–375. [PubMed] [Google Scholar]
  17. Rabinovsky E. D., Yang T. J. Production and characterization of a bovine T cell-specific monoclonal antibody identifying a mature differentiation antigen. J Immunol. 1986 Jan;136(2):609–615. [PubMed] [Google Scholar]
  18. Reinherz E. L., Schlossman S. F. The differentiation and function of human T lymphocytes. Cell. 1980 Apr;19(4):821–827. doi: 10.1016/0092-8674(80)90072-0. [DOI] [PubMed] [Google Scholar]
  19. Sayre P. H., Chang H. C., Hussey R. E., Brown N. R., Richardson N. E., Spagnoli G., Clayton L. K., Reinherz E. L. Molecular cloning and expression of T11 cDNAs reveal a receptor-like structure on human T lymphocytes. Proc Natl Acad Sci U S A. 1987 May;84(9):2941–2945. doi: 10.1073/pnas.84.9.2941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sewell W. A., Brown M. H., Owen M. J., Fink P. J., Kozak C. A., Crumpton M. J. The murine homologue of the T lymphocyte CD2 antigen: molecular cloning, chromosome assignment and cell surface expression. Eur J Immunol. 1987 Jul;17(7):1015–1020. doi: 10.1002/eji.1830170718. [DOI] [PubMed] [Google Scholar]
  21. Stitz L., Althage A., Hengartner H., Zinkernagel R. Natural killer cells vs cytotoxic T cells in the peripheral blood of virus-infected mice. J Immunol. 1985 Jan;134(1):598–602. [PubMed] [Google Scholar]
  22. Williams A. F., Barclay A. N., Clark S. J., Paterson D. J., Willis A. C. Similarities in sequences and cellular expression between rat CD2 and CD4 antigens. J Exp Med. 1987 Feb 1;165(2):368–380. doi: 10.1084/jem.165.2.368. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES