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. 1989 Jun;67(2):205–209.

Oestradiol suppression of delayed-type hypersensitivity in autoimmune (NZB/NZW)F1 mice is a trait inherited from the healthy NZW parental strain.

H Carlsten 1, R Holmdahl 1, A Tarkowski 1, L A Nilsson 1
PMCID: PMC1385258  PMID: 2753520

Abstract

In this paper we report that treatment with 17 beta-oestradiol depresses cutaneous delayed-type hypersensitivity (DTH) to oxazolone (OXA) in female oophorectomized NZB/W mice. Analysis of the parental strains revealed that this oestrogen-induced inhibition of DTH mice is a trait inherited from the healthy NZW and not from the autoimmune NZB strain. The down-regulating effect of oestradiol on DTH in female NZB/W mice was found both in the sensitization and effector phase of the DTH response. Decreased DTH reactivity to OXA was demonstrated in oophorectomized NZB/W and NZW mice after administration of both low, physiological doses (s.c. injections) and high, pharmacological doses (siliac tube implantation) of oestradiol. In addition, s.c. implantation of testosterone containing siliac tubes suppressed DTH reactivity to OXA in castrated male NZW mice. This down-regulating effect was not found in male NZB or NZB/W mice similarly treated. Serological analysis revealed that high doses of oestradiol increased whereas testosterone depressed the IgG anti-OXA antibody responses in female NZB/W mice. Our results indicate that one possible contribution of the NZW parental strain to the lupus disease in NZB/W mice is a trait of oestrogen sensitivity leading to the acceleration of the autoimmune disease, the latter feature being inherited from NZB mice.

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Selected References

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  1. Boilert B., Edqvist L. E., Johansson E. D., Lindberg P., Martinsson K. The influence of conjugated estrogens in radioimmunoassays using different antibodies against estradiol-17beta. Steroids. 1973 Dec;22(6):891–894. doi: 10.1016/0039-128x(73)90060-3. [DOI] [PubMed] [Google Scholar]
  2. Carlsten H., Nilsson L. A., Tarkowski A. Impaired cutaneous delayed-type hypersensitivity in autoimmune MRL lpr/lpr mice. Int Arch Allergy Appl Immunol. 1986;81(4):322–325. doi: 10.1159/000234156. [DOI] [PubMed] [Google Scholar]
  3. Edqvist L. E., Johansson E. D. Radioimmunoassay of oestrone and oestradiol in human and bovine peripheral plasma. Acta Endocrinol (Copenh) 1972 Dec;71(4):716–730. doi: 10.1530/acta.0.0710716. [DOI] [PubMed] [Google Scholar]
  4. Grossman C. J. Interactions between the gonadal steroids and the immune system. Science. 1985 Jan 18;227(4684):257–261. doi: 10.1126/science.3871252. [DOI] [PubMed] [Google Scholar]
  5. Holmdahl R., Jansson L., Meyerson B., Klareskog L. Oestrogen induced suppression of collagen arthritis: I. Long term oestradiol treatment of DBA/1 mice reduces severity and incidence of arthritis and decreases the anti type II collagen immune response. Clin Exp Immunol. 1987 Nov;70(2):372–378. [PMC free article] [PubMed] [Google Scholar]
  6. Howie J. B., Helyer B. J. The immunology and pathology of NZB mice. Adv Immunol. 1968;9:215–266. doi: 10.1016/s0065-2776(08)60444-7. [DOI] [PubMed] [Google Scholar]
  7. Jacob C. O., McDevitt H. O. Tumour necrosis factor-alpha in murine autoimmune 'lupus' nephritis. Nature. 1988 Jan 28;331(6154):356–358. doi: 10.1038/331356a0. [DOI] [PubMed] [Google Scholar]
  8. Kotzin B. L., Palmer E. The contribution of NZW genes to lupus-like disease in (NZB x NZW)F1 mice. J Exp Med. 1987 May 1;165(5):1237–1251. doi: 10.1084/jem.165.5.1237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lahita R. G. Sex steroids and the rheumatic diseases. Arthritis Rheum. 1985 Feb;28(2):121–126. doi: 10.1002/art.1780280202. [DOI] [PubMed] [Google Scholar]
  10. Lycke N., Lindholm L., Holmgren J. Cholera antibody production in vitro by peripheral blood lymphocytes following oral immunization of humans and mice. Clin Exp Immunol. 1985 Oct;62(1):39–47. [PMC free article] [PubMed] [Google Scholar]
  11. Näkelä O., Kaartinen M., Pelkonen J. L., Karjalainen K. Inheritance of antibody specificity V. Anti-2-phenyloxazolone in the mouse. J Exp Med. 1978 Dec 1;148(6):1644–1660. doi: 10.1084/jem.148.6.1644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Roubinian J. R., Talal N., Greenspan J. S., Goodman J. R., Siiteri P. K. Effect of castration and sex hormone treatment on survival, anti-nucleic acid antibodies, and glomerulonephritis in NZB/NZW F1 mice. J Exp Med. 1978 Jun 1;147(6):1568–1583. doi: 10.1084/jem.147.6.1568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sivam G., Cohen M. S., Dodd R. C., Gray T. K. 17 Beta-estradiol inhibits the oxidative metabolism of U937 cells indirectly via lymphocytes. Endocrinology. 1987 Sep;121(3):853–857. doi: 10.1210/endo-121-3-853. [DOI] [PubMed] [Google Scholar]
  14. Steinberg A. D., Melez K. A., Raveche E. S., Reeves J. P., Boegel W. A., Smathers P. A., Taurog J. D., Weinlein L., Duvic M. Approach to the study of the role of sex hormones in autoimmunity. Arthritis Rheum. 1979 Nov;22(11):1170–1176. doi: 10.1002/art.1780221103. [DOI] [PubMed] [Google Scholar]
  15. Theofilopoulos A. N., Dixon F. J. Etiopathogenesis of murine SLE. Immunol Rev. 1981;55:179–216. doi: 10.1111/j.1600-065x.1981.tb00343.x. [DOI] [PubMed] [Google Scholar]
  16. Van Loveren H., Kato K., Ratzlaff R. E., Meade R., Ptak W., Askenase P. W. Use of micrometers and calipers to measure various components of delayed-type hypersensitivity ear swelling reactions in mice. J Immunol Methods. 1984 Mar 16;67(2):311–319. doi: 10.1016/0022-1759(84)90471-x. [DOI] [PubMed] [Google Scholar]
  17. Zinkernagel R. M., Dixon F. J. Comparison of T cell-mediated immune responsiveness of NZB, (NZB x &NZW)F1 hybrid and other murine strains. Clin Exp Immunol. 1977 Jul;29(1):110–121. [PMC free article] [PubMed] [Google Scholar]

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