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. 1989 Jun;67(2):237–243.

Recombinant IL-4 and IFN-gamma activate locomotor capacity in human B lymphocytes.

P C Wilkinson 1, L N Islam 1
PMCID: PMC1385264  PMID: 2502496

Abstract

Recombinant human interleukin-4 (IL-4) or interferon-gamma (IFN-gamma) were added to cultures of B-enriched human lymphocytes from normal blood, or to the lymphocytes from five patients with chronic lymphocytic leukaemia (CLL). IL-4 and IFN-gamma caused the B lymphocytes to acquire locomotor capacity, as judged by morphological polarization and invasion of collagen gels. This was detectable in normal B cells within a few hours of culture and fully developed by 24-48 hr. It was inhibited by the presence of cyclosporin A. The responding, motile cells also increased in size. These findings suggest that B lymphocytes acquire locomotor capacity early in growth, as the cells move from G0 to G1. IL-4 or IFN-gamma had no direct effect in polarizing lymphocytes in a short-term (30-min) assay, thus they do not behave like chemotactic factors. They slowly increase the proportion of locomotor cells in B-lymphocyte populations, and these motile cells respond by polarization to factors released by the growing cells into the medium.

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Selected References

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  1. Boyd A. W., Tedder T. F., Griffin J. D., Freedman A. S., Fisher D. C., Daley J., Nadler L. M. Preexposure of resting B cells to interferon-gamma enhances their proliferative response to subsequent activation signals. Cell Immunol. 1987 May;106(2):355–365. doi: 10.1016/0008-8749(87)90178-x. [DOI] [PubMed] [Google Scholar]
  2. Dunn G. A., Brown A. F. Alignment of fibroblasts on grooved surfaces described by a simple geometric transformation. J Cell Sci. 1986 Jul;83:313–340. doi: 10.1242/jcs.83.1.313. [DOI] [PubMed] [Google Scholar]
  3. Gordon J., Millsum M. J., Guy G. R., Ledbetter J. A. Resting B lymphocytes can be triggered directly through the CDw40 (Bp50) antigen. A comparison with IL-4-mediated signaling. J Immunol. 1988 Mar 1;140(5):1425–1430. [PubMed] [Google Scholar]
  4. Harris P., Ralph P. Human leukemic models of myelomonocytic development: a review of the HL-60 and U937 cell lines. J Leukoc Biol. 1985 Apr;37(4):407–422. doi: 10.1002/jlb.37.4.407. [DOI] [PubMed] [Google Scholar]
  5. Haston W. S. F-actin distribution in polymorphonuclear leucocytes. J Cell Sci. 1987 Nov;88(Pt 4):495–501. doi: 10.1242/jcs.88.4.495. [DOI] [PubMed] [Google Scholar]
  6. Haston W. S., Shields J. M., Wilkinson P. C. Lymphocyte locomotion and attachment on two-dimensional surfaces and in three-dimensional matrices. J Cell Biol. 1982 Mar;92(3):747–752. doi: 10.1083/jcb.92.3.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jarvis S. C., Snyderman R., Cohen H. J. Human lymphocyte motility: normal characteristics and anomalous behavior of chronic lymphocytic leukemia cells. Blood. 1976 Nov;48(5):717–729. [PubMed] [Google Scholar]
  8. Keller H. U., Wilkinson P. C., Abercrombie M., Becker E. L., Hirsch J. G., Miller M. E., Scottramsey W., Zigmond S. H. A proposal for the definition of terms related to locomotion of leucocytes and other cells. Clin Exp Immunol. 1977 Mar;27(3):377–380. [PMC free article] [PubMed] [Google Scholar]
  9. Klaus G. G., Hawrylowicz C. M. Activation and proliferation signals in mouse B cells. II. Evidence for activation (G0 to G1) signals differing in sensitivity to cyclosporine. Eur J Immunol. 1984 Mar;14(3):250–254. doi: 10.1002/eji.1830140309. [DOI] [PubMed] [Google Scholar]
  10. Kornfeld H., Berman J. S., Beer D. J., Center D. M. Induction of human T lymphocyte motility by interleukin 2. J Immunol. 1985 Jun;134(6):3887–3890. [PubMed] [Google Scholar]
  11. Kornfeld H., Cruikshank W. W., Pyle S. W., Berman J. S., Center D. M. Lymphocyte activation by HIV-1 envelope glycoprotein. Nature. 1988 Sep 29;335(6189):445–448. doi: 10.1038/335445a0. [DOI] [PubMed] [Google Scholar]
  12. McGregor D. D., Logie P. S. The mediator of cellular immunity. VII. Localization of sensitized lymphocytes in inflammatory exudates. J Exp Med. 1974 Jun 1;139(6):1415–1430. doi: 10.1084/jem.139.6.1415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Potter J. W., Van Epps D. E. Separation and purification of lymphocyte chemotactic factor (LCF) and interleukin 2 produced by human peripheral blood mononuclear cells. Cell Immunol. 1987 Mar;105(1):9–22. doi: 10.1016/0008-8749(87)90051-7. [DOI] [PubMed] [Google Scholar]
  14. Romagnani S., Giudizi M. G., Biagiotti R., Almerigogna F., Mingari C., Maggi E., Liang C. M., Moretta L. B cell growth factor activity of interferon-gamma. Recombinant human interferon-gamma promotes proliferation of anti-mu-activated human B lymphocytes. J Immunol. 1986 May 15;136(10):3513–3516. [PubMed] [Google Scholar]
  15. Sundqvist K. G., Otteskog P. Anchorage and lymphocyte function: collagen and the maintenance of motile shape in T cells. Immunology. 1986 Jul;58(3):365–369. [PMC free article] [PubMed] [Google Scholar]
  16. Walker L., Guy G., Brown G., Rowe M., Milner A. E., Gordon J. Control of human B-lymphocyte replication. I. Characterization of novel activation states that precede the entry of G0 B cells into cycle. Immunology. 1986 Aug;58(4):583–589. [PMC free article] [PubMed] [Google Scholar]
  17. Wilkinson P. C., Higgins A. Cyclosporin A inhibits mitogen-activated but not phorbol ester-activated locomotion of human lymphocytes. Immunology. 1987 Jul;61(3):311–316. [PMC free article] [PubMed] [Google Scholar]
  18. Wilkinson P. C., Higgins A. OKT3-activated locomotion of human blood lymphocytes: a phenomenon requiring contact of T cells with Fc receptor-bearing cells. Immunology. 1987 Mar;60(3):445–451. [PMC free article] [PubMed] [Google Scholar]
  19. Wilkinson P. C., Islam L. N., Sinclair D., Dagg J. H. The defect of lymphocyte locomotion in chronic lymphocytic leukaemia: studies of polarization and growth-dependent locomotion. Clin Exp Immunol. 1988 Mar;71(3):497–501. [PMC free article] [PubMed] [Google Scholar]
  20. Wilkinson P. C., Lackie J. M., Haston W. S., Islam L. N. Effects of phorbol esters on shape and locomotion of human blood lymphocytes. J Cell Sci. 1988 Aug;90(Pt 4):645–655. doi: 10.1242/jcs.90.4.645. [DOI] [PubMed] [Google Scholar]
  21. Wilkinson P. C. The locomotor capacity of human lymphocytes and its enhancement by cell growth. Immunology. 1986 Feb;57(2):281–289. [PMC free article] [PubMed] [Google Scholar]

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