Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1989 Jun;67(2):237–243.

Recombinant IL-4 and IFN-gamma activate locomotor capacity in human B lymphocytes.

P C Wilkinson 1, L N Islam 1
PMCID: PMC1385264  PMID: 2502496

Abstract

Recombinant human interleukin-4 (IL-4) or interferon-gamma (IFN-gamma) were added to cultures of B-enriched human lymphocytes from normal blood, or to the lymphocytes from five patients with chronic lymphocytic leukaemia (CLL). IL-4 and IFN-gamma caused the B lymphocytes to acquire locomotor capacity, as judged by morphological polarization and invasion of collagen gels. This was detectable in normal B cells within a few hours of culture and fully developed by 24-48 hr. It was inhibited by the presence of cyclosporin A. The responding, motile cells also increased in size. These findings suggest that B lymphocytes acquire locomotor capacity early in growth, as the cells move from G0 to G1. IL-4 or IFN-gamma had no direct effect in polarizing lymphocytes in a short-term (30-min) assay, thus they do not behave like chemotactic factors. They slowly increase the proportion of locomotor cells in B-lymphocyte populations, and these motile cells respond by polarization to factors released by the growing cells into the medium.

Full text

PDF
237

Images in this article

238Figure 1
on p.238

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyd A. W., Tedder T. F., Griffin J. D., Freedman A. S., Fisher D. C., Daley J., Nadler L. M. Preexposure of resting B cells to interferon-gamma enhances their proliferative response to subsequent activation signals. Cell Immunol. 1987 May;106(2):355–365. doi: 10.1016/0008-8749(87)90178-x. [DOI] [PubMed] [Google Scholar]
  2. Dunn G. A., Brown A. F. Alignment of fibroblasts on grooved surfaces described by a simple geometric transformation. J Cell Sci. 1986 Jul;83:313–340. doi: 10.1242/jcs.83.1.313. [DOI] [PubMed] [Google Scholar]
  3. Gordon J., Millsum M. J., Guy G. R., Ledbetter J. A. Resting B lymphocytes can be triggered directly through the CDw40 (Bp50) antigen. A comparison with IL-4-mediated signaling. J Immunol. 1988 Mar 1;140(5):1425–1430. [PubMed] [Google Scholar]
  4. Harris P., Ralph P. Human leukemic models of myelomonocytic development: a review of the HL-60 and U937 cell lines. J Leukoc Biol. 1985 Apr;37(4):407–422. doi: 10.1002/jlb.37.4.407. [DOI] [PubMed] [Google Scholar]
  5. Haston W. S. F-actin distribution in polymorphonuclear leucocytes. J Cell Sci. 1987 Nov;88(Pt 4):495–501. doi: 10.1242/jcs.88.4.495. [DOI] [PubMed] [Google Scholar]
  6. Haston W. S., Shields J. M., Wilkinson P. C. Lymphocyte locomotion and attachment on two-dimensional surfaces and in three-dimensional matrices. J Cell Biol. 1982 Mar;92(3):747–752. doi: 10.1083/jcb.92.3.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jarvis S. C., Snyderman R., Cohen H. J. Human lymphocyte motility: normal characteristics and anomalous behavior of chronic lymphocytic leukemia cells. Blood. 1976 Nov;48(5):717–729. [PubMed] [Google Scholar]
  8. Keller H. U., Wilkinson P. C., Abercrombie M., Becker E. L., Hirsch J. G., Miller M. E., Scottramsey W., Zigmond S. H. A proposal for the definition of terms related to locomotion of leucocytes and other cells. Clin Exp Immunol. 1977 Mar;27(3):377–380. [PMC free article] [PubMed] [Google Scholar]
  9. Klaus G. G., Hawrylowicz C. M. Activation and proliferation signals in mouse B cells. II. Evidence for activation (G0 to G1) signals differing in sensitivity to cyclosporine. Eur J Immunol. 1984 Mar;14(3):250–254. doi: 10.1002/eji.1830140309. [DOI] [PubMed] [Google Scholar]
  10. Kornfeld H., Berman J. S., Beer D. J., Center D. M. Induction of human T lymphocyte motility by interleukin 2. J Immunol. 1985 Jun;134(6):3887–3890. [PubMed] [Google Scholar]
  11. Kornfeld H., Cruikshank W. W., Pyle S. W., Berman J. S., Center D. M. Lymphocyte activation by HIV-1 envelope glycoprotein. Nature. 1988 Sep 29;335(6189):445–448. doi: 10.1038/335445a0. [DOI] [PubMed] [Google Scholar]
  12. McGregor D. D., Logie P. S. The mediator of cellular immunity. VII. Localization of sensitized lymphocytes in inflammatory exudates. J Exp Med. 1974 Jun 1;139(6):1415–1430. doi: 10.1084/jem.139.6.1415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Potter J. W., Van Epps D. E. Separation and purification of lymphocyte chemotactic factor (LCF) and interleukin 2 produced by human peripheral blood mononuclear cells. Cell Immunol. 1987 Mar;105(1):9–22. doi: 10.1016/0008-8749(87)90051-7. [DOI] [PubMed] [Google Scholar]
  14. Romagnani S., Giudizi M. G., Biagiotti R., Almerigogna F., Mingari C., Maggi E., Liang C. M., Moretta L. B cell growth factor activity of interferon-gamma. Recombinant human interferon-gamma promotes proliferation of anti-mu-activated human B lymphocytes. J Immunol. 1986 May 15;136(10):3513–3516. [PubMed] [Google Scholar]
  15. Sundqvist K. G., Otteskog P. Anchorage and lymphocyte function: collagen and the maintenance of motile shape in T cells. Immunology. 1986 Jul;58(3):365–369. [PMC free article] [PubMed] [Google Scholar]
  16. Walker L., Guy G., Brown G., Rowe M., Milner A. E., Gordon J. Control of human B-lymphocyte replication. I. Characterization of novel activation states that precede the entry of G0 B cells into cycle. Immunology. 1986 Aug;58(4):583–589. [PMC free article] [PubMed] [Google Scholar]
  17. Wilkinson P. C., Higgins A. Cyclosporin A inhibits mitogen-activated but not phorbol ester-activated locomotion of human lymphocytes. Immunology. 1987 Jul;61(3):311–316. [PMC free article] [PubMed] [Google Scholar]
  18. Wilkinson P. C., Higgins A. OKT3-activated locomotion of human blood lymphocytes: a phenomenon requiring contact of T cells with Fc receptor-bearing cells. Immunology. 1987 Mar;60(3):445–451. [PMC free article] [PubMed] [Google Scholar]
  19. Wilkinson P. C., Islam L. N., Sinclair D., Dagg J. H. The defect of lymphocyte locomotion in chronic lymphocytic leukaemia: studies of polarization and growth-dependent locomotion. Clin Exp Immunol. 1988 Mar;71(3):497–501. [PMC free article] [PubMed] [Google Scholar]
  20. Wilkinson P. C., Lackie J. M., Haston W. S., Islam L. N. Effects of phorbol esters on shape and locomotion of human blood lymphocytes. J Cell Sci. 1988 Aug;90(Pt 4):645–655. doi: 10.1242/jcs.90.4.645. [DOI] [PubMed] [Google Scholar]
  21. Wilkinson P. C. The locomotor capacity of human lymphocytes and its enhancement by cell growth. Immunology. 1986 Feb;57(2):281–289. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES