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. 1989 May;67(1):38–43.

Obligatory role of macrophages in dengue virus antigen presentation to B lymphocytes.

N Rizvi 1, U C Chaturvedi 1, A Mathur 1
PMCID: PMC1385285  PMID: 2500392

Abstract

The study was undertaken to investigate the role of dengue type 2 virus (DV)-infected mouse peritoneal macrophages (M phi) in presentation of the DV antigen to B lymphocytes as shown by counting virus-specific IgM antibody plaque-forming cells (PFC). It was observed that heat-killed or glutaraldehyde-fixed M phi did not present the antigen. Pretreatment of M phi with the lysosomotropic compounds ammonium chloride and chloroquine inhibited the antigen presentation. Depletion of M phi from the spleen cell cultures abrogated the immune response to DV. The tryptic-digested DV antigen could stimulate immune responses in B-lymphocyte enriched (depleted of M phi and T cells) spleen cell cultures, and the digested antigen could be presented by glutaraldehyde-fixed M phi. Pretreatment of M phi with a trypsin inhibitor abrogated antigen presentation. The findings thus show that even for presentation to B cells the DV antigen must be processed by M phi by a trypsin-like protease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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