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. 1989 May;67(1):96–102.

Immunoregulatory properties of bone marrow-derived cells in the iris and ciliary body.

J S Williamson 1, D Bradley 1, J W Streilein 1
PMCID: PMC1385295  PMID: 2525521

Abstract

Iris and ciliary body of mouse eyes have been examined for the presence of bone marrow-derived cells possessing the capability of functioning as antigen-presenting cells (APC). We have determined that iris and ciliary body contain significant numbers of cells bearing T200, indicating their bone marrow origin. Most of these express the F4/80 marker typically found on mature macrophages. However, approximately one-third of the cells express Ia and a similar number express Mac-1 markers. Virtually none of the cells express Thy-1 or surface immunoglobulin. Whole preparations of excised iris/ciliary body, or single cell suspensions prepared from these tissues were then assayed for their capacity to induce proliferation among allogeneic lymphocytes. It was discovered that iris/ciliary body tissues or cells did not function as alloantigen-presenting cells, although tissue and cells derived from the corneal limbus were allostimulatory. In addition, iris/ciliary body tissues and cells displayed the ability to suppress mixed lymphocyte reactions to which they had been added as regulatory cells. We conclude that normal iris and ciliary body contain bone marrow-derived cells that fail to function as alloantigen-presenting cells. However, cells were present that have the capacity to inhibit alloimmune lymphocyte proliferation. The strategic location of inhibitory cells in the tissues that line the anterior chamber of the eye raises the possibility that these cells may play a role in the phenomenon of immunological privilege that is characteristic of this site.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austyn J. M., Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur J Immunol. 1981 Oct;11(10):805–815. doi: 10.1002/eji.1830111013. [DOI] [PubMed] [Google Scholar]
  2. Austyn J. M. Lymphoid dendritic cells. Immunology. 1987 Oct;62(2):161–170. [PMC free article] [PubMed] [Google Scholar]
  3. Barker C. F., Billingham R. E. Immunologically privileged sites. Adv Immunol. 1977;25:1–54. [PubMed] [Google Scholar]
  4. Bergstresser P. R., Toews G. B., Gilliam J. N., Streilein J. W. Unusual numbers and distribution of Langerhans cells in skin with unique immunologic properties. J Invest Dermatol. 1980 May;74(5):312–314. doi: 10.1111/1523-1747.ep12543542. [DOI] [PubMed] [Google Scholar]
  5. Caspi R. R., Roberge F. G., Nussenblatt R. B. Organ-resident, nonlymphoid cells suppress proliferation of autoimmune T-helper lymphocytes. Science. 1987 Aug 28;237(4818):1029–1032. doi: 10.1126/science.2956685. [DOI] [PubMed] [Google Scholar]
  6. Claman H. N., Miller S. D., Sy M. S., Moorhead J. W. Suppressive mechanisms involving sensitization and tolerance in contact allergy. Immunol Rev. 1980;50:105–132. doi: 10.1111/j.1600-065x.1980.tb00309.x. [DOI] [PubMed] [Google Scholar]
  7. Ferguson T. A., Waldrep J. C., Kaplan H. J. The immune response and the eye. II. The nature of T suppressor cell induction in anterior chamber-associated immune deviation (ACAID). J Immunol. 1987 Jul 15;139(2):352–357. [PubMed] [Google Scholar]
  8. Geppert T. D., Lipsky P. E. Antigen presentation by interferon-gamma-treated endothelial cells and fibroblasts: differential ability to function as antigen-presenting cells despite comparable Ia expression. J Immunol. 1985 Dec;135(6):3750–3762. [PubMed] [Google Scholar]
  9. Gillette T. E., Chandler J. W. Immunofluorescence and histochemistry of corneal epithelial flat mounts: use of EDTA. Curr Eye Res. 1981;1(4):249–253. doi: 10.3109/02713688109001855. [DOI] [PubMed] [Google Scholar]
  10. Goodwin J. S., DeHoratius R., Israel H., Peake G. T., Messner R. P. Suppressor cell function in sarcoidosis. Ann Intern Med. 1979 Feb;90(2):169–173. doi: 10.7326/0003-4819-90-2-169. [DOI] [PubMed] [Google Scholar]
  11. Hillinger S. M., Herzig G. P. Impaired cell-mediated immunity in Hodgkin's disease mediated by suppressor lymphocytes and monocytes. J Clin Invest. 1978 Jun;61(6):1620–1627. doi: 10.1172/JCI109082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Holt P. G., Degebrodt A., O'Leary C., Krska K., Plozza T. T cell activation by antigen-presenting cells from lung tissue digests: suppression by endogenous macrophages. Clin Exp Immunol. 1985 Dec;62(3):586–593. [PMC free article] [PubMed] [Google Scholar]
  13. Inaba K., Schuler G., Witmer M. D., Valinksy J., Atassi B., Steinman R. M. Immunologic properties of purified epidermal Langerhans cells. Distinct requirements for stimulation of unprimed and sensitized T lymphocytes. J Exp Med. 1986 Aug 1;164(2):605–613. doi: 10.1084/jem.164.2.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ju S. T., Dorf M. E. Functional analysis of cloned macrophage hybridomas. IV. Induction and inhibition of mixed lymphocyte responses. J Immunol. 1985 Jun;134(6):3722–3730. [PubMed] [Google Scholar]
  15. Klareskog L., Forsum U., Tjernlund U. M., Rask L., Peterson P. A. Expression of Ia antigen-like molecules on cells in the corneal epithelium. Invest Ophthalmol Vis Sci. 1979 Mar;18(3):310–313. [PubMed] [Google Scholar]
  16. Le J. M., Vilcek J. Accessory function of human fibroblasts in mitogen-stimulated interferon-gamma production by T lymphocytes. Inhibition by interleukin 1 and tumor necrosis factor. J Immunol. 1987 Nov 15;139(10):3330–3337. [PubMed] [Google Scholar]
  17. Linden J. Enhanced cAMP accumulation after termination of cholinergic action in the heart. FASEB J. 1987 Aug;1(2):119–124. doi: 10.1096/fasebj.1.2.2440752. [DOI] [PubMed] [Google Scholar]
  18. Lynch M. G., Peeler J. S., Brown R. H., Niederkorn J. Y. Expression of HLA class I and II antigens on cells of the human trabecular meshwork. Ophthalmology. 1987 Jul;94(7):851–857. doi: 10.1016/s0161-6420(87)33539-0. [DOI] [PubMed] [Google Scholar]
  19. Markenson J. A., Morgan J. W., Lockshin M. D., Joachim C., Winfield J. B. Responses of fractionated cells from patients with systemic lupus erythematosus and normals to plant mitogen: evidence for a suppressor population of monocytes. Proc Soc Exp Biol Med. 1978 May;158(1):5–9. doi: 10.3181/00379727-158-40127. [DOI] [PubMed] [Google Scholar]
  20. Maurer D. H., Collins W. E., Hanke J. H., Van M., Rich R. R., Pollack M. S. Class II positive human dermal fibroblasts restimulate cloned allospecific T cells but fail to stimulate primary allogeneic lymphoproliferation. Hum Immunol. 1985 Nov;14(3):245–258. doi: 10.1016/0198-8859(85)90232-0. [DOI] [PubMed] [Google Scholar]
  21. Niederkorn J., Streilein J. W., Shadduck J. A. Deviant immune responses to allogeneic tumors injected intracamerally and subcutaneously in mice. Invest Ophthalmol Vis Sci. 1981 Mar;20(3):355–363. [PubMed] [Google Scholar]
  22. Omary M. B., Trowbridge I. S., Scheid M. P. T200 cell surface glycoprotein of the mouse. Polymorphism defined by the Ly-5 system of alloantigens. J Exp Med. 1980 May 1;151(5):1311–1316. doi: 10.1084/jem.151.5.1311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Poulter L. W. Antigen presenting cells in situ: their identification and involvement in immunopathology. Clin Exp Immunol. 1983 Sep;53(3):513–520. [PMC free article] [PubMed] [Google Scholar]
  24. Rodrigues M. M., Rowden G., Hackett J., Bakos I. Langerhans cells in the normal conjunctiva and peripheral cornea of selected species. Invest Ophthalmol Vis Sci. 1981 Nov;21(5):759–765. [PubMed] [Google Scholar]
  25. Rosenthal A. S., Shevach E. M. Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes. J Exp Med. 1973 Nov 1;138(5):1194–1212. doi: 10.1084/jem.138.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Springer T., Galfré G., Secher D. S., Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979 Apr;9(4):301–306. doi: 10.1002/eji.1830090410. [DOI] [PubMed] [Google Scholar]
  27. Steinman R. M., Gutchinov B., Witmer M. D., Nussenzweig M. C. Dendritic cells are the principal stimulators of the primary mixed leukocyte reaction in mice. J Exp Med. 1983 Feb 1;157(2):613–627. doi: 10.1084/jem.157.2.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Treseler P. A., Sanfilippo F. The expression of major histocompatibility complex and leukocyte antigens by cells in the rat cornea. Transplantation. 1986 Feb;41(2):248–252. doi: 10.1097/00007890-198602000-00022. [DOI] [PubMed] [Google Scholar]
  29. Trowbridge I. S. Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200. J Exp Med. 1978 Jul 1;148(1):313–323. doi: 10.1084/jem.148.1.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Tsujisaki M., Igarashi M., Sakaguchi K., Eisinger M., Herlyn M., Ferrone S. Immunochemical and functional analysis of HLA class II antigens induced by recombinant immune interferon on normal epidermal melanocytes. J Immunol. 1987 Feb 15;138(4):1310–1316. [PubMed] [Google Scholar]
  31. Wang H. M., Kaplan H. J., Chan W. C., Johnson M. The distribution and ontogeny of MHC antigens in murine ocular tissue. Invest Ophthalmol Vis Sci. 1987 Aug;28(8):1383–1389. [PubMed] [Google Scholar]

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