Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1989 Jul;67(3):365–369.

Flare-up reaction on murine contact hypersensitivity. I. Description of an experimental model: rechallenge system.

N Yamashita 1, M Natsuaki 1, S Sagami 1
PMCID: PMC1385354  PMID: 2759658

Abstract

In this experiment, a 'rechallenge system' was established in BALB/c mice to study a local immunological reaction of contact hypersensitivity (CH). Briefly, mice were sensitized by a single painting with 25 microliters of 0.5% dinitrofluorobenzene (DNFB) on the shaved back skin on Day 0. On Day 5, they were challenged with 20 microliters of 0.2% DNFB on each left ear, and on Day 33 challenged again with either painting 20 microliters of 0.2% DNFB on their both ears or intravenous administration of 15 mg of dinitrobenzene sulphonic acid (DNBS). As the result, marked ear swelling was observed by the second challenge only on the first challenged site and these responses were clarified to be antigen specific. In in vitro experiments, it was shown that only the cells from the regional lymph node of the skin, which were previously elicited on challenge, were enhanced to proliferate by DNBS, which was added into the culture medium. These results suggest that there is a local immunological mechanism to respond to the specific antigen only on the site in which CH reaction has been elicited previously. The results from this 'rechallenge system' may help to explain some pathological mechanisms of such chronic diseases as fixed drug eruption or chronic contact dermatitis, which recur easily in skin lesions involved previously.

Full text

PDF
365

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asherson G. L., Ptak W. Contact and delayed hypersensitivity in the mouse. I. Active sensitization and passive transfer. Immunology. 1968 Sep;15(3):405–416. [PMC free article] [PubMed] [Google Scholar]
  2. Chase M. W. Developments in delayed-type hypersensitivities: 1950-1975. J Invest Dermatol. 1976 Jul;67(1):136–148. doi: 10.1111/1523-1747.ep12513002. [DOI] [PubMed] [Google Scholar]
  3. Claman H. N., Miller S. D., Conlon P. J., Moorhead J. W. Control of experimental contact sensitivity. Adv Immunol. 1980;30:121–157. doi: 10.1016/s0065-2776(08)60195-9. [DOI] [PubMed] [Google Scholar]
  4. Claman H. N., Miller S. D., Sy M. S., Moorhead J. W. Suppressive mechanisms involving sensitization and tolerance in contact allergy. Immunol Rev. 1980;50:105–132. doi: 10.1111/j.1600-065x.1980.tb00309.x. [DOI] [PubMed] [Google Scholar]
  5. Crowle A. J. Delayed hypersensitivity in the mouse. Adv Immunol. 1975;20:197–264. doi: 10.1016/s0065-2776(08)60209-6. [DOI] [PubMed] [Google Scholar]
  6. Dahlbäck K., Möller H. Flare-up of contract dermatitis to picryl chloride in the mouse. Acta Derm Venereol. 1981;61(4):342–344. [PubMed] [Google Scholar]
  7. Klasen I. S., van der Kwast T. H., Donselaar I. G., Ladestein R. M., Benner R. Flare-up of delayed-type hypersensitivity initially induced by murine cloned helper T cells. Cell Immunol. 1987 Aug;108(1):235–241. doi: 10.1016/0008-8749(87)90208-5. [DOI] [PubMed] [Google Scholar]
  8. Lens J. W., van den Berg W. B., van de Putte L. B., van den Bersselaar L. Flare-up of antigen-induced arthritis in mice after challenge with oral antigen. Clin Exp Immunol. 1984 Nov;58(2):364–371. [PMC free article] [PubMed] [Google Scholar]
  9. Moorhead J. W. Tolerance and contact sensitivity to DNFA in mice. VIII. Identification of distinct T cell subpopulations that mediate in vivo and in vitro manifestations of delayed hypersensitivity. J Immunol. 1978 Jan;120(1):137–144. [PubMed] [Google Scholar]
  10. Scheper R. J., von Blomberg M., Boerrigter G. H., Bruynzeel D., van Dinther A., Vos A. Induction of immunological memory in the skin. Role of local T cell retention. Clin Exp Immunol. 1983 Jan;51(1):141–148. [PMC free article] [PubMed] [Google Scholar]
  11. van Maarsseveen A. C., Bomhof G., Scheper R. J. Demonstration of accelerated and increased migration inhibition factor release in vivo in a PPD retest reaction. Int Arch Allergy Appl Immunol. 1982;67(1):1–6. doi: 10.1159/000232979. [DOI] [PubMed] [Google Scholar]
  12. van de Putte L. B., Lens J. W., van den Berg W. B., Kruijsen M. W. Exacerbation of antigen-induced arthritis after challenge with intravenous antigen. Immunology. 1983 May;49(1):161–167. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES