Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1989 Jul;67(3):394–400.

Confirmation of destruction of salmonellae within murine peritoneal exudate cells by immunocytochemical technique.

F R Lin 1, H S Hsu 1, V R Mumaw 1, C W Moncure 1
PMCID: PMC1385359  PMID: 2668159

Abstract

A procedure was developed with which peritoneal exudate cell (PEC) preparations were fixed in a glutaraldehyde-picric acid mixture, post-fixed with osmium tetroxide, embedded in LR White resin and then stained with immunogold probe. It provided tissue sections showing both well-defined ultrastructures as well as specifically labelled Salmonella O antigens by electron microscopy. Inbred, male C57BL/6 mice were injected intraperitoneally with 2 x 10(7) virulent Salmonella typhimurium. Peritoneal exudate cells were harvested at 16 and 20 hr after infection. Disintegrating intracellular bacteria were identified as salmonellae by the immunogold markers. Deposition of gold particles in the cytoplasm of phagocytes also indicated that intracellular debris contained digested pathogen. This investigation therefore confirms previous findings of the destruction of salmonellae within inflammatory polymorphs and macrophages.

Full text

PDF
394

Images in this article

395Figure 1
on p.395
396Figure 2
on p.396
396Figure 3
on p.396
396Figure 4
on p.396
397Figure 5
on p.397
397Figure 6
on p.397
398Figure 7
on p.398
398Figure 8
on p.398

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Collins F. M. Vaccines and cell-mediated immunity. Bacteriol Rev. 1974 Dec;38(4):371–402. doi: 10.1128/br.38.4.371-402.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Faulk W. P., Taylor G. M. An immunocolloid method for the electron microscope. Immunochemistry. 1971 Nov;8(11):1081–1083. doi: 10.1016/0019-2791(71)90496-4. [DOI] [PubMed] [Google Scholar]
  3. Guo Y. N., Hsu H. S., Mumaw V. R., Nakoneczna I. Electronmicroscopy studies on the bactericidal action of inflammatory leukocytes in murine salmonellosis. J Med Microbiol. 1986 Mar;21(2):151–159. doi: 10.1099/00222615-21-2-151. [DOI] [PubMed] [Google Scholar]
  4. Guo Y. N., Hsu H. S., Mumaw V. R., Nakoneczna I. Electronmicroscopy studies on the opsonic role of antiserum and the subsequent destruction of Salmonellae within murine inflammatory leukocytes. J Med Microbiol. 1986 Dec;22(4):343–349. doi: 10.1099/00222615-22-4-343. [DOI] [PubMed] [Google Scholar]
  5. Hsu H. S., Mayo D. R. Interactions between macrophages of guinea pigs and salmonellae. 3. Bactericidal action and cytophilic antibodies of macrophages of infected guinea pigs. Infect Immun. 1973 Aug;8(2):165–172. doi: 10.1128/iai.8.2.165-172.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hsu H. S., Radcliffe A. S. Interactions between macrophages of guinea pigs and Salmonellae. I. Fate of Salmonella typhimurium within macrophages of normal guinea pigs. J Bacteriol. 1968 Jul;96(1):191–197. doi: 10.1128/jb.96.1.191-197.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lin F. R., Wang X. M., Hsu H. S., Mumaw V. R., Nakoneczna I. Electron microscopic studies on the location of bacterial proliferation in the liver in murine salmonellosis. Br J Exp Pathol. 1987 Aug;68(4):539–550. [PMC free article] [PubMed] [Google Scholar]
  8. Marecki N. M., Hsu H. S., Mayo D. R. Cellular and humoral aspects of host resistance in murine salmonellosis. Br J Exp Pathol. 1975 Jun;56(3):231–243. [PMC free article] [PubMed] [Google Scholar]
  9. Nakoneczna I., Hsu H. S. Histopathological study of protective immunity against murine salmonellosis induced by killed vaccine. Infect Immun. 1983 Jan;39(1):423–430. doi: 10.1128/iai.39.1.423-430.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nakoneczna I., Hsu H. S. The comparative histopathology of primary and secondary lesions in murine salmonellosis. Br J Exp Pathol. 1980 Feb;61(1):76–84. [PMC free article] [PubMed] [Google Scholar]
  11. Newman G. R., Jasani B., Williams E. D. A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope. Histochem J. 1983 Jun;15(6):543–555. doi: 10.1007/BF01954145. [DOI] [PubMed] [Google Scholar]
  12. Rhodes M. W., Hsu H. S. Effect of kanamycin on the fate of Salmonella enteritidis within cultured macrophages of guinea pigs. J Reticuloendothel Soc. 1974 Jan;15(1):1–12. [PubMed] [Google Scholar]
  13. Romano E. L., Stolinski C., Hughes-Jones N. C. An antiglobulin reagent labelled with colloidal gold for use in electron microscopy. Immunochemistry. 1974 Aug;11(8):521–522. doi: 10.1016/0019-2791(74)90162-1. [DOI] [PubMed] [Google Scholar]
  14. Roth J., Bendayan M., Carlemalm E., Villiger W., Garavito M. Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J Histochem Cytochem. 1981 May;29(5):663–671. doi: 10.1177/29.5.6166664. [DOI] [PubMed] [Google Scholar]
  15. SUTER E. Interaction between phagocytes and pathogenic microorganisms. Bacteriol Rev. 1956 Jun;20(2):94–132. doi: 10.1128/br.20.2.94-132.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wang X. M., Lin F. R., Hsu H. S., Mumaw V. R., Nakoneczna I. Electronmicroscopic studies on the location of salmonella proliferation in the murine spleen. J Med Microbiol. 1988 Jan;25(1):41–47. doi: 10.1099/00222615-25-1-41. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES