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. 1989 Oct;68(2):221–226.

Interferon-gamma-increased adherence of lymphocytes to high endothelial venules.

H R Hendriks 1, C Korn 1, R E Mebius 1, G Kraal 1
PMCID: PMC1385421  PMID: 2509328

Abstract

Recirculating lymphocytes specifically adhere to and migrate through endothelium lining high endothelial venules (HEV), using specific and non-specific receptor-ligand systems. Interferons (IFN) profoundly affect the traffic of lymphocytes. Therefore the effects of interferon-gamma (IFN-gamma) on lymphocytes with respect to their interaction with HEV endothelium in lymph nodes were studied using the frozen section assay. In addition the organ-specificity of T- and B-lymphocyte adherence to HEV was investigated. Lymphocytes from spleen and peripheral lymph nodes showed increased binding to HEV of up to 35% compared to control lymphocytes after in vivo administration of IFN-gamma. The specificity of their binding did not change in terms of T:B-cell ratios. Increased adherence was also found by preincubation of lymphocytes with IFN-gamma in The enhanced binding was not due to increased expression of Mel-14 homing receptors and LFA-1 molecules on T and B lymphocytes, as shown by fluorescence-activated cell sorter analysis and antibody-blocking studies in the frozen section assay. Apparently IFN-gamma induces an additional Mel-14-independent and LFA-1-independent adhesion mechanism on lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altieri D. C., Edgington T. S. A monoclonal antibody reacting with distinct adhesion molecules defines a transition in the functional state of the receptor CD11b/CD18 (Mac-1). J Immunol. 1988 Oct 15;141(8):2656–2660. [PubMed] [Google Scholar]
  2. Burmester G. R., Strobel G. F., Göhring P., Kalden J. R. Adhesion of human T lymphocytes to endothelial cells isolated from the umbilical vein: II. Enhanced binding of in vitro activated T lymphocytes. Immunobiology. 1987 Nov;175(5):394–405. doi: 10.1016/S0171-2985(87)80068-2. [DOI] [PubMed] [Google Scholar]
  3. Butcher E. C., Scollay R. G., Weissman I. L. Lymphocyte adherence to high endothelial venules: characterization of a modified in vitro assay, and examination of the binding of syngeneic and allogeneic lymphocyte populations. J Immunol. 1979 Nov;123(5):1996–2003. [PubMed] [Google Scholar]
  4. Butcher E. C. The regulation of lymphocyte traffic. Curr Top Microbiol Immunol. 1986;128:85–122. doi: 10.1007/978-3-642-71272-2_3. [DOI] [PubMed] [Google Scholar]
  5. Chang E. H., Jay F. T., Friedman R. M. Physical, morphological, and biochemical alterations in the membrane of AKR mouse cells after interferon treatment. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1859–1863. doi: 10.1073/pnas.75.4.1859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dustin M. L., Springer T. A. Lymphocyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells. J Cell Biol. 1988 Jul;107(1):321–331. doi: 10.1083/jcb.107.1.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Friedman R. M., Vogel S. N. Interferons with special emphasis on the immune system. Adv Immunol. 1983;34:97–140. doi: 10.1016/s0065-2776(08)60378-8. [DOI] [PubMed] [Google Scholar]
  8. Gresser I., Guy-Grand D., Maury C., Maunoury M. T. Interferon induces peripheral lymphadenopathy in mice. J Immunol. 1981 Oct;127(4):1569–1575. [PubMed] [Google Scholar]
  9. Hamann A., Jablonski-Westrich D., Duijvestijn A., Butcher E. C., Baisch H., Harder R., Thiele H. G. Evidence for an accessory role of LFA-1 in lymphocyte-high endothelium interaction during homing. J Immunol. 1988 Feb 1;140(3):693–699. [PubMed] [Google Scholar]
  10. Hein W. R., Supersaxo A. Effect of interferon-alpha-2a on the output of recirculating lymphocytes from single lymph nodes. Immunology. 1988 Jul;64(3):469–474. [PMC free article] [PubMed] [Google Scholar]
  11. Hendriks H. R., Duijvestijn A. M., Kraal G. Rapid decrease in lymphocyte adherence to high endothelial venules in lymph nodes deprived of afferent lymphatic vessels. Eur J Immunol. 1987 Dec;17(12):1691–1695. doi: 10.1002/eji.1830171203. [DOI] [PubMed] [Google Scholar]
  12. Hendriks H. R., Eestermans I. L. Disappearance and reappearance of high endothelial venules and immigrating lymphocytes in lymph nodes deprived of afferent lymphatic vessels: a possible regulatory role of macrophages in lymphocyte migration. Eur J Immunol. 1983 Aug;13(8):663–669. doi: 10.1002/eji.1830130811. [DOI] [PubMed] [Google Scholar]
  13. Issekutz T. B., Stoltz J. M., vd Meide P. Lymphocyte recruitment in delayed-type hypersensitivity. The role of IFN-gamma. J Immunol. 1988 May 1;140(9):2989–2993. [PubMed] [Google Scholar]
  14. Kimber I., Sparshott S. M., Bell E. B., Ford W. L. The effects of interferon on the recirculation of lymphocytes in the rat. Immunology. 1987 Apr;60(4):585–591. [PMC free article] [PubMed] [Google Scholar]
  15. Knight E., Jr, Korant B. D. A cell surface alteration in mouse L cells induced by interferon. Biochem Biophys Res Commun. 1977 Jan 24;74(2):707–713. doi: 10.1016/0006-291x(77)90360-6. [DOI] [PubMed] [Google Scholar]
  16. Korngold R., Blank K. J., Murasko D. M. Effect of interferon on thoracic duct lymphocyte output: induction with either poly I:poly C or vaccinia virus. J Immunol. 1983 May;130(5):2236–2240. [PubMed] [Google Scholar]
  17. Kraal G., Duijvestijn A. M., Hendriks H. H. The endothelium of the high endothelial venule: a specialized endothelium with unique properties. Exp Cell Biol. 1987;55(1):1–10. doi: 10.1159/000163388. [DOI] [PubMed] [Google Scholar]
  18. Kraal G., Weissman I. L., Butcher E. C. Memory B cells express a phenotype consistent with migratory competence after secondary but not short-term primary immunization. Cell Immunol. 1988 Aug;115(1):78–87. doi: 10.1016/0008-8749(88)90163-3. [DOI] [PubMed] [Google Scholar]
  19. Ledbetter J. A., Herzenberg L. A. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63–90. doi: 10.1111/j.1600-065x.1979.tb00289.x. [DOI] [PubMed] [Google Scholar]
  20. Martz E. LFA-1 and other accessory molecules functioning in adhesions of T and B lymphocytes. Hum Immunol. 1987 Jan;18(1):3–37. doi: 10.1016/0198-8859(87)90110-8. [DOI] [PubMed] [Google Scholar]
  21. Mountford C. E., Wright L. C. Organization of lipids in the plasma membranes of malignant and stimulated cells: a new model. Trends Biochem Sci. 1988 May;13(5):172–177. doi: 10.1016/0968-0004(88)90145-4. [DOI] [PubMed] [Google Scholar]
  22. Pals S. T., den Otter A., Miedema F., Kabel P., Keizer G. D., Scheper R. J., Meijer C. J. Evidence that leukocyte function-associated antigen-1 is involved in recirculation and homing of human lymphocytes via high endothelial venules. J Immunol. 1988 Mar 15;140(6):1851–1853. [PubMed] [Google Scholar]
  23. Pfeffer L. M., Wang E., Tamm I. Interferon effects on microfilament organization, cellular fibronectin distribution, and cell motility in human fibroblasts. J Cell Biol. 1980 Apr;85(1):9–17. doi: 10.1083/jcb.85.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Pierres A., Naquet P., Van Agthoven A., Bekkhoucha F., Denizot F., Mishal Z., Schmitt-Verhulst A. M., Pierres M. A rat anti-mouse T4 monoclonal antibody (H129.19) inhibits the proliferation of Ia-reactive T cell clones and delineates two phenotypically distinct (T4+, Lyt-2,3-, and T4-, Lyt-2,3+) subsets among anti-Ia cytolytic T cell clones. J Immunol. 1984 Jun;132(6):2775–2782. [PubMed] [Google Scholar]
  25. Reichert R. A., Gallatin W. M., Weissman I. L., Butcher E. C. Germinal center B cells lack homing receptors necessary for normal lymphocyte recirculation. J Exp Med. 1983 Mar 1;157(3):813–827. doi: 10.1084/jem.157.3.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Roozemond R. C., Bonavida B. Effect of altered membrane fluidity on NK cell-mediated cytotoxicity. I. Selective inhibition of the recognition or post recognition events in the cytolytic pathway of NK cells. J Immunol. 1985 Apr;134(4):2209–2214. [PubMed] [Google Scholar]
  27. Schattner A., Meshorer A., Wallach D. Involvement of interferon in virus-induced lymphopenia. Cell Immunol. 1983 Jul 1;79(1):11–25. doi: 10.1016/0008-8749(83)90046-1. [DOI] [PubMed] [Google Scholar]
  28. Siegelman M., Bond M. W., Gallatin W. M., St John T., Smith H. T., Fried V. A., Weissman I. L. Cell surface molecule associated with lymphocyte homing is a ubiquitinated branched-chain glycoprotein. Science. 1986 Feb 21;231(4740):823–829. doi: 10.1126/science.3003913. [DOI] [PubMed] [Google Scholar]
  29. Springer T. A., Dustin M. L., Kishimoto T. K., Marlin S. D. The lymphocyte function-associated LFA-1, CD2, and LFA-3 molecules: cell adhesion receptors of the immune system. Annu Rev Immunol. 1987;5:223–252. doi: 10.1146/annurev.iy.05.040187.001255. [DOI] [PubMed] [Google Scholar]
  30. Stamper H. B., Jr, Woodruff J. J. Lymphocyte homing into lymph nodes: in vitro demonstration of the selective affinity of recirculating lymphocytes for high-endothelial venules. J Exp Med. 1976 Sep 1;144(3):828–833. doi: 10.1084/jem.144.3.828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Woodruff J. J., Clarke L. M., Chin Y. H. Specific cell-adhesion mechanisms determining migration pathways of recirculating lymphocytes. Annu Rev Immunol. 1987;5:201–222. doi: 10.1146/annurev.iy.05.040187.001221. [DOI] [PubMed] [Google Scholar]
  32. Yu C. L., Haskard D. O., Cavender D., Johnson A. R., Ziff M. Human gamma interferon increases the binding of T lymphocytes to endothelial cells. Clin Exp Immunol. 1985 Dec;62(3):554–560. [PMC free article] [PubMed] [Google Scholar]
  33. van der Meide P. H., Dubbeld M., Vijverberg K., Kos T., Schellekens H. The purification and characterization of rat gamma interferon by use of two monoclonal antibodies. J Gen Virol. 1986 Jun;67(Pt 6):1059–1071. doi: 10.1099/0022-1317-67-6-1059. [DOI] [PubMed] [Google Scholar]

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