Abstract
The mechanisms which generate heterogeneity amongst resident tissue macrophages (M phi) are poorly understood. In a previous study we described a novel mouse M phi haemagglutinin, which binds unopsonized sheep erythrocytes. This sheep erythrocyte receptor (SER) is expressed at high levels on stromal M phi from bone marrow and lymph nodes, but at low levels on M phi from serous cavities and broncho-alveolar spaces. In this paper we demonstrate that a species-restricted factor in mouse serum is required in vitro for optimal maintenance of SER on resident bone marrow M phi and for its induction on M phi populations which normally lack this receptor. Using thioglycollate-elicited peritoneal M phi, induction of SER by mouse serum was dose-dependent, reached maximal levels by 3-4 days, required the continuous presence of mouse serum, and was fully reversible. Re-expression following trypsinization was inhibited by cycloheximide, showing that protein synthesis by M phi was necessary. Using a quantitative microtitre plate assay to measure levels of the inducing activity (SER-IA) in different samples, it was found to be heat-labile, non-dialysable, precipitable by polyethylene glycol and inactivated at pH 4 but not at pH 9.6. On gel filtration of mouse serum, a single major peak of activity was obtained with an apparent MW of around 70,000. SER-IA appears to be unrelated to a variety of factors and cytokines which affect M phi function, including colony-stimulating factor-1 (CSF-1). The possible role of SER-IA in regulating the differential expression of SER in vivo is discussed.
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- Crocker P. R., Gordon S. Properties and distribution of a lectin-like hemagglutinin differentially expressed by murine stromal tissue macrophages. J Exp Med. 1986 Dec 1;164(6):1862–1875. doi: 10.1084/jem.164.6.1862. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dijkstra C. D., Döpp E. A., Joling P., Kraal G. The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology. 1985 Mar;54(3):589–599. [PMC free article] [PubMed] [Google Scholar]
- Harmsen A. G., Muggenburg B. A., Snipes M. B., Bice D. E. The role of macrophages in particle translocation from lungs to lymph nodes. Science. 1985 Dec 13;230(4731):1277–1280. doi: 10.1126/science.4071052. [DOI] [PubMed] [Google Scholar]
- Humphrey J. H., Sundaram V. Origin and turnover of follicular dendritic cells and marginal zone macrophages in the mouse spleen. Adv Exp Med Biol. 1985;186:167–170. doi: 10.1007/978-1-4613-2463-8_21. [DOI] [PubMed] [Google Scholar]
- Stanley E. R., Heard P. M. Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells. J Biol Chem. 1977 Jun 25;252(12):4305–4312. [PubMed] [Google Scholar]
- Steinman R. M., Nogueira N., Witmer M. D., Tydings J. D., Mellman I. S. Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages. J Exp Med. 1980 Nov 1;152(5):1248–1261. doi: 10.1084/jem.152.5.1248. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Unkeless J. C., Fleit H., Mellman I. S. Structural Aspects and Heterogeneity of Immunoglobulin Fc Receptors. Adv Immunol. 1981;31:247–270. doi: 10.1016/s0065-2776(08)60922-0. [DOI] [PubMed] [Google Scholar]
- van Furth R., Cohn Z. A. The origin and kinetics of mononuclear phagocytes. J Exp Med. 1968 Sep 1;128(3):415–435. doi: 10.1084/jem.128.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]