Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1988 Dec;65(4):629–634.

Effect of Ly 5 allotype on in vitro immune responses.

R E Taffs 1, S J Ewald 1
PMCID: PMC1385575  PMID: 2975635

Abstract

We have tested in vitro immune responses of several kinds to determine if Ly 5 allotype influences reactivity of murine splenocytes in processes thought to involve the T200 glycoprotein. Matings were established among C57BL/6J (B6) (Ly 5.1) and C57BL/6-Ly 5.2 (B6-Ly 5.2) congenic mice (both H-2b) to obtain sibling mice segregating for alloalleles of the Ly 5 system. F2 progeny of the three Ly 5 genotypes were tested for antibody-dependent cell-mediated cytotoxicity (ADCC), proliferation in allogeneic mixed lymphocyte culture (MLC), mitogen responsiveness, natural killer (NK) cell activity, and cytotoxic T-lymphocyte (CTL)-mediated cytotoxic activity. We observed that in Ly 5 segregant mice, higher CTL activity was associated with the Ly 5.2 type. No allotype effect was observed in MLC, mitogen responses, and NK cell-mediated cytolysis. In the parental and F1 animals, mice carrying the Ly 5.2 allele had significantly higher ADCC levels, though this effect was not seen in the segregants. Our results indicate that Ly 5 or some closely linked gene or genes influence CTL activity of murine splenocytes in vitro.

Full text

PDF
629

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Decker J. M., Warr G. W., Marchalonis J. J. A membrane glycoprotein which binds antilymphocyte globulin and concanavalin A is implicated in stimulation of murine T lymphocytes. Biochem Biophys Res Commun. 1977 Feb 21;74(4):1536–1543. doi: 10.1016/0006-291x(77)90617-9. [DOI] [PubMed] [Google Scholar]
  2. Harp J. A., Davis B. S., Ewald S. J. Inhibition of T cell responses to alloantigens and polyclonal mitogens by Ly-5 antisera. J Immunol. 1984 Jul;133(1):10–15. [PubMed] [Google Scholar]
  3. Harp J. A., Ewald S. J. Modulation of in vitro immune responses by monoclonal antibody to T200 antigen. Cell Immunol. 1983 Oct 1;81(1):71–80. doi: 10.1016/0008-8749(83)90212-5. [DOI] [PubMed] [Google Scholar]
  4. Herberman R. B., Nunn M. E., Holden H. T., Staal S., Djeu J. Y. Augmentation of natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic target cells. Int J Cancer. 1977 Apr 15;19(4):555–564. doi: 10.1002/ijc.2910190417. [DOI] [PubMed] [Google Scholar]
  5. Lefrançois L., Bevan M. J. Novel antigenic determinants of the T200 glycoprotein expressed preferentially by activated cytotoxic T lymphocytes. J Immunol. 1985 Jul;135(1):374–383. [PubMed] [Google Scholar]
  6. Martorell J., Vilella R., Borche L., Rojo I., Vives J. A second signal for T cell mitogenesis provided by monoclonal antibodies CD45 (T200). Eur J Immunol. 1987 Oct;17(10):1447–1451. doi: 10.1002/eji.1830171010. [DOI] [PubMed] [Google Scholar]
  7. Morrison R. P., Nishio J., Chesebro B. Influence of the murine MHC (H-2) on Friend leukemia virus-induced immunosuppression. J Exp Med. 1986 Feb 1;163(2):301–314. doi: 10.1084/jem.163.2.301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nakayama E., Shiku H., Stockert E., Oettgen H. F., Old L. J. Cytotoxic T cells: Lyt phenotype and blocking of killing activity by Lyt antisera. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1977–1981. doi: 10.1073/pnas.76.4.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Newman W., Fast L. D., Rose L. M. Blockade of NK cell lysis is a property of monoclonal antibodies that bind to distinct regions of T-200. J Immunol. 1983 Oct;131(4):1742–1747. [PubMed] [Google Scholar]
  10. Omary M. B., Trowbridge I. S., Scheid M. P. T200 cell surface glycoprotein of the mouse. Polymorphism defined by the Ly-5 system of alloantigens. J Exp Med. 1980 May 1;151(5):1311–1316. doi: 10.1084/jem.151.5.1311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ralph S. J., Berridge M. V. Expression of antigens of the 'T200' family of glycoproteins on hemopoietic stem cells: evidence that thymocyte cell lineage antigens are represented on 'T200'. J Immunol. 1984 May;132(5):2510–2514. [PubMed] [Google Scholar]
  12. Saga Y., Tung J. S., Shen F. W., Boyse E. A. Alternative use of 5' exons in the specification of Ly-5 isoforms distinguishing hematopoietic cell lineages. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5364–5368. doi: 10.1073/pnas.84.15.5364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Salisbury J. G., Banga J. P. Lentil lectin binding properties of the leucocyte common antigen from rat dividing and non-dividing thymocytes. Thymus. 1985;7(6):335–344. [PubMed] [Google Scholar]
  14. Schindler L., Engler H., Kirchner H. Activation of natural killer cells and induction of interferon after injection of mouse hepatitis virus type 3 in mice. Infect Immun. 1982 Mar;35(3):869–873. doi: 10.1128/iai.35.3.869-873.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shen F. W., Saga Y., Litman G., Freeman G., Tung J. S., Cantor H., Boyse E. A. Cloning of Ly-5 cDNA. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7360–7363. doi: 10.1073/pnas.82.21.7360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Siadak A. W., Nowinski R. C. Identification of Ly-5 and T200 antigens on identical cell surface proteins. J Immunol. 1980 Sep;125(3):1400–1401. [PubMed] [Google Scholar]
  17. Small R. M., Walden S. M., Ewald S. J. Effects of Ly-5 antibodies on antibody-dependent cell-mediated cytotoxicity (ADCC). Immunology. 1987 Feb;60(2):159–165. [PMC free article] [PubMed] [Google Scholar]
  18. Targan S. R., Newman W. Definition of a "trigger" stage in the NK cytolytic reaction sequence by a monoclonal antibody to the glycoprotein T-200. J Immunol. 1983 Sep;131(3):1149–1153. [PubMed] [Google Scholar]
  19. Thomas M. L., Reynolds P. J., Chain A., Ben-Neriah Y., Trowbridge I. S. B-cell variant of mouse T200 (Ly-5): evidence for alternative mRNA splicing. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5360–5363. doi: 10.1073/pnas.84.15.5360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tung J. S., Scheid M. P., Pierotti M. A., Hämmerling U., Boyse E. A. Structural features and selective expression of three Ly-5+ cell-surface molecules. Immunogenetics. 1981;14(1-2):101–106. doi: 10.1007/BF00344303. [DOI] [PubMed] [Google Scholar]
  21. Yakura H., Shen F. W., Bourcet E., Boyse E. A. On the function of Ly-5 in the regulation of antigen-driven B cell differentiation. Comparison and contrast with Lyb-2. J Exp Med. 1983 Apr 1;157(4):1077–1088. doi: 10.1084/jem.157.4.1077. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES