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. 1988 Dec;65(4):651–653.

Activated adherent large granular lymphocytes/natural killer (LGL/NK) cells change their migratory behaviour.

A Pirelli 1, P Allavena 1, A Mantovani 1
PMCID: PMC1385579  PMID: 3220508

Abstract

Unstimulated large granular lymphocytes/natural killer (LGL/NK) cells, unlike small T lymphocytes, exhibit prompt locomotion into nitrocellulose filters in response to chemo-attactrants, but, unlike monocytes, are unable to migrate as adherent cells across polycarbonate filters. Upon activation with 4-B-phorbol 12,13 dibutyrate (PDBU), LGL/NK cells become adherent and change their migratory behaviour, having the ability to migrate as adherent cells across polycarbonate filters. PDBU-treated high-density T lymphocytes did not show, under the same conditions, locomotory activity. The change in migratory behaviour following activation may represent an important determinant of the ability of activated LGL/NK cells to adhere to vascular linings and localize in tissues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Argov S., Hebdon M., Cuatrecasas P., Koren H. S. Phorbol ester-induced lymphocyte adherence: selective action on NK cells. J Immunol. 1985 Apr;134(4):2215–2222. [PubMed] [Google Scholar]
  2. Bottazzi B., Introna M., Allavena P., Villa A., Mantovani A. In vitro migration of human large granular lymphocytes. J Immunol. 1985 Apr;134(4):2316–2321. [PubMed] [Google Scholar]
  3. Colotta F., Peri G., Villa A., Mantovani A. Rapid killing of actinomycin D-treated tumor cells by human mononuclear cells. I. Effectors belong to the monocyte-macrophage lineage. J Immunol. 1984 Feb;132(2):936–944. [PubMed] [Google Scholar]
  4. Natuk R. J., Welsh R. M. Accumulation and chemotaxis of natural killer/large granular lymphocytes at sites of virus replication. J Immunol. 1987 Feb 1;138(3):877–883. [PubMed] [Google Scholar]
  5. Pohajdak B., Gomez J., Orr F. W., Khalil N., Talgoy M., Greenberg A. H. Chemotaxis of large granular lymphocytes. J Immunol. 1986 Jan;136(1):278–284. [PubMed] [Google Scholar]
  6. Polentarutti N., Bottazzi B., Balotta C., Erroi A., Mantovani A. Modulation of the locomotory capacity of human large granular lymphocytes. Cell Immunol. 1986 Aug;101(1):204–212. doi: 10.1016/0008-8749(86)90198-x. [DOI] [PubMed] [Google Scholar]
  7. Ramos O. F., Masucci M. G., Klein E. Modulation of human blood lymphocyte cytotoxicity by the phorbol ester tumor promoter P(Bu)2: increase of target binding, impairment of effector recycling, and activation of lytic potential which is independent of IL-2. Cell Immunol. 1985 Mar;91(1):178–192. doi: 10.1016/0008-8749(85)90042-5. [DOI] [PubMed] [Google Scholar]
  8. Timonen T., Saksela E. Isolation of human NK cells by density gradient centrifugation. J Immunol Methods. 1980;36(3-4):285–291. doi: 10.1016/0022-1759(80)90133-7. [DOI] [PubMed] [Google Scholar]
  9. Trinchieri G., O'Brien T., Shade M., Perussia B. Phorbol esters enhance spontaneous cytotoxicity of human lymphocytes, abrogate Fc receptor expression, and inhibit antibody-dependent lymphocyte-mediated cytotoxicity. J Immunol. 1984 Oct;133(4):1869–1877. [PubMed] [Google Scholar]
  10. Trinchieri G., Perussia B. Human natural killer cells: biologic and pathologic aspects. Lab Invest. 1984 May;50(5):489–513. [PubMed] [Google Scholar]
  11. Wilkinson P. C., Haston W. S., Shields J. M. Some determinants of the locomotory behaviour of phagocytes and lymphocytes in vitro. Clin Exp Immunol. 1982 Dec;50(3):461–473. [PMC free article] [PubMed] [Google Scholar]

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