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. 1990 Mar;69(3):391–395.

Determination of the molecular nature and cellular localization of Thy-1 in human renal tissue

T Miyata, K Isobe, R Dawson, M A Ritter, R Inagi, O Oda, R Taguchi, H Ikezawa, I Inoue, H Seo, M Hasegawa, S Kobayashi, K Maeda, K Yamada, I Nakashima
PMCID: PMC1385957  PMID: 1968887

Abstract

The molecular nature and cellular localization of Thy-1 antigen in human renal tissue were studied. Strong immunohistochemical staining was observed in frozen sections of human kidney using monoclonal anti-human Thy-1 antibody; this reaction was almost completely abolished by pretreating the kidney section with phosphatidyl inositol (PI)-specific phospholipase C (PI-PLC). Immunohistochemical analysis revealed that the Thy-1 antigen is localized on the proximal tubular epithelial cells and the Bowman's capsule of the glomerulus. Northern blot analysis of renal mRNA using a cloned human Thy-1 gene revealed the presence of human Thy-1 mRNA of a similar size to the one in human brain. When a human kidney cDNA library was screened with the same probe, a cDNA of human Thy-1 was isolated. Moreover, human Thy-1 protein with a molecular weight (MW) of 21,000 was detected in renal tissue by gel electrophoresis and Western blot analysis using monoclonal anti-human Thy-1 antibody. These data demonstrate for the first time the production of human Thy-1 as a PI-anchored protein with a unique cellular location in human renal tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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