Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1990 Mar;69(3):490–493.

Patterns of nuclear proto-oncogene expression during induced differentiation and proliferation of human B chronic lymphocytic leukaemia cells.

J J Murphy 1, M Tracz 1, J D Norton 1
PMCID: PMC1385973  PMID: 2312171

Abstract

Phorbol ester-induced differentiation of human B-chronic lymphocytic leukaemic cells was found to be preceded by a rapid transient induction in expression of the c-jun proto-oncogene, which paralleled that of c-fos. Induced expression of c-myc but not of c-fos/c-jun proto-oncogenes was markedly higher in a proliferating variant leukaemic cell population compared with that seen in typical lymphocytic leukaemia cells. These data suggest that the c-fos/c-jun nuclear oncogenes play a role in induced differentiation, whilst c-myc is more important in the proliferative response of B lymphocytes.

Full text

PDF
490

Images in this article

492Figure 2
on p.492

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alitalo K., Schwab M., Lin C. C., Varmus H. E., Bishop J. M. Homogeneously staining chromosomal regions contain amplified copies of an abundantly expressed cellular oncogene (c-myc) in malignant neuroendocrine cells from a human colon carcinoma. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1707–1711. doi: 10.1073/pnas.80.6.1707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Catovsky D., Cherchi M., Brookss D., Bradely J., Zola H. Heterogeneity of B-cell leukemias demonstrated by the monoclonal antibody FMC7. Blood. 1981 Aug;58(2):406–408. [PubMed] [Google Scholar]
  3. Cossman J., Neckers L. M., Braziel R. M., Trepel J. B., Korsmeyer S. J., Bakhshi A. In vitro enhancement of immunoglobulin gene expression in chronic lymphocytic leukemia. J Clin Invest. 1984 Feb;73(2):587–592. doi: 10.1172/JCI111247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Curran T., Franza B. R., Jr Fos and Jun: the AP-1 connection. Cell. 1988 Nov 4;55(3):395–397. doi: 10.1016/0092-8674(88)90024-4. [DOI] [PubMed] [Google Scholar]
  5. Gordon J., Mellstedt H., Aman P., Biberfeld P., Klein G. Phenotypic modulation of chronic lymphocytic leukemia cells by phorbol ester: induction of IgM secretion and changes in the expression of B cell-associated surface antigens. J Immunol. 1984 Jan;132(1):541–547. [PubMed] [Google Scholar]
  6. Heikkila R., Schwab G., Wickstrom E., Loke S. L., Pluznik D. H., Watt R., Neckers L. M. A c-myc antisense oligodeoxynucleotide inhibits entry into S phase but not progress from G0 to G1. 1987 Jul 30-Aug 5Nature. 328(6129):445–449. doi: 10.1038/328445a0. [DOI] [PubMed] [Google Scholar]
  7. Iguchi-Ariga S. M., Itani T., Kiji Y., Ariga H. Possible function of the c-myc product: promotion of cellular DNA replication. EMBO J. 1987 Aug;6(8):2365–2371. doi: 10.1002/j.1460-2075.1987.tb02513.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Larsson L. G., Gray H. E., Tötterman T., Pettersson U., Nilsson K. Drastically increased expression of MYC and FOS protooncogenes during in vitro differentiation of chronic lymphocytic leukemia cells. Proc Natl Acad Sci U S A. 1987 Jan;84(1):223–227. doi: 10.1073/pnas.84.1.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Murphy J. J., Tooze J., Millard R. E., Hudson L. Analysis and isolation of leukaemic and residual normal B lymphocyte populations from patients with chronic lymphocytic leukaemia. Clin Exp Immunol. 1987 Jun;68(3):669–676. [PMC free article] [PubMed] [Google Scholar]
  10. Norton J. D., Leber B., Yaxley J. C. Patterns of gene expression during plasmacytoid differentiation of chronic lymphocytic leukaemia cells. FEBS Lett. 1987 May 4;215(1):127–131. doi: 10.1016/0014-5793(87)80126-6. [DOI] [PubMed] [Google Scholar]
  11. Reed J. C., Alpers J. D., Nowell P. C., Hoover R. G. Sequential expression of protooncogenes during lectin-stimulated mitogenesis of normal human lymphocytes. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3982–3986. doi: 10.1073/pnas.83.11.3982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ryseck R. P., Hirai S. I., Yaniv M., Bravo R. Transcriptional activation of c-jun during the G0/G1 transition in mouse fibroblasts. Nature. 1988 Aug 11;334(6182):535–537. doi: 10.1038/334535a0. [DOI] [PubMed] [Google Scholar]
  13. Studzinski G. P., Brelvi Z. S., Feldman S. C., Watt R. A. Participation of c-myc protein in DNA synthesis of human cells. Science. 1986 Oct 24;234(4775):467–470. doi: 10.1126/science.3532322. [DOI] [PubMed] [Google Scholar]
  14. Tötterman T. H., Nilsson K., Sundström C. Phorbol ester-induced differentiation of chronic lymphocytic leukaemia cells. Nature. 1980 Nov 13;288(5787):176–178. doi: 10.1038/288176a0. [DOI] [PubMed] [Google Scholar]
  15. de Taisne C., Gegonne A., Stehelin D., Bernheim A., Berger R. Chromosomal localization of the human proto-oncogene c-ets. Nature. 1984 Aug 16;310(5978):581–583. doi: 10.1038/310581a0. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES