Abstract
Three physiological states of a single bacterial strain, namely, balanced, phosphorus-rich, and nitrogen-rich bacteria, were obtained by culturing a bacterial strain in chemostats under three different nutrient regimens. Each was shown to be distinctly different in elemental composition with respect to C/N/P ratio. These bacteria were fed to four species of heterotrophic nanoflagellates in batch culture grazing experiments, and the percent regeneration efficiencies of bacterium-bound nitrogen and phosphorus by the flagellates were compared. All flagellate species regenerated comparable amounts of nitrogen, which was thought to be due to their similar internal C/N ratios. There was, however, interspecies variation with regard to phosphorus regeneration: the two faster-growing species (Paraphysomonas imperforata and Bodo designis) released significantly more phosphorus than the two slower-growing species (Stephanoeca diplocostata and Jakoba libera). The observed differences were thought to have been influenced by a combination of life cycle strategies and internal C/P ratios.
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Selected References
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- Bloem J., Starink M., Bär-Gilissen M. J., Cappenberg T. E. Protozoan grazing, bacterial activity, and mineralization in two-stage continuous cultures. Appl Environ Microbiol. 1988 Dec;54(12):3113–3121. doi: 10.1128/aem.54.12.3113-3121.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bratbak G. Bacterial biovolume and biomass estimations. Appl Environ Microbiol. 1985 Jun;49(6):1488–1493. doi: 10.1128/aem.49.6.1488-1493.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kulaev I. S., Vagabov V. M. Polyphosphate metabolism in micro-organisms. Adv Microb Physiol. 1983;24:83–171. doi: 10.1016/s0065-2911(08)60385-9. [DOI] [PubMed] [Google Scholar]
- Sherr B. F., Sherr E. B., Berman T. Grazing, growth, and ammonium excretion rates of a heterotrophic microflagellate fed with four species of bacteria. Appl Environ Microbiol. 1983 Apr;45(4):1196–1201. doi: 10.1128/aem.45.4.1196-1201.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]