Abstract
We used restriction fragment length polymorphisms to examine mitochondrial genome rearrangements in 36 wild strains of the cultivated basidiomycete Agrocybe aegerita, collected from widely distributed locations in Europe. We identified two polymorphic regions within the mitochondrial DNA which varied independently: one carrying the Cox II coding sequence and the other carrying the Cox I, ATP6, and ATP8 coding sequences. Two types of mutations were responsible for the restriction fragment length polymorphisms that we observed and, accordingly, were involved in the A. aegerita mitochondrial genome evolution: (i) point mutations, which resulted in strain-specific mitochondrial markers, and (ii) length mutations due to genome rearrangements, such as deletions, insertions, or duplications. Within each polymorphic region, the length differences defined only two mitochondrial types, suggesting that these length mutations were not randomly generated but resulted from a precise rearrangement mechanism. For each of the two polymorphic regions, the two molecular types were distributed among the 36 strains without obvious correlation with their geographic origin. On the basis of these two polymorphisms, it is possible to define four mitochondrial haplotypes. The four mitochondrial haplotypes could be the result of intermolecular recombination between allelic forms present in the population long enough to reach linkage equilibrium. All of the 36 dikaryotic strains contained only a single mitochondrial type, confirming the previously described mitochondrial sorting out after cytoplasmic mixing in basidiomycetes.
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Selected References
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- Barroso G., Moulinier T., Labarère J. Involvement of a large inverted repeated sequence in a recombinational rearrangement of the mitochondrial genome of the higher fungus Agrocybe aegerita. Curr Genet. 1992 Aug;22(2):155–161. doi: 10.1007/BF00351476. [DOI] [PubMed] [Google Scholar]
- Brown W. M., Prager E. M., Wang A., Wilson A. C. Mitochondrial DNA sequences of primates: tempo and mode of evolution. J Mol Evol. 1982;18(4):225–239. doi: 10.1007/BF01734101. [DOI] [PubMed] [Google Scholar]
- Collins R. A., Lambowitz A. M. Structural variations and optional introns in the mitochondrial DNAs of Neurospora strains isolated from nature. Plasmid. 1983 Jan;9(1):53–70. doi: 10.1016/0147-619x(83)90031-8. [DOI] [PubMed] [Google Scholar]
- Cummings D. J., MacNeil I. A., Domenico J., Matsuura E. T. Excision-amplification of mitochondrial DNA during senescence in Podospora anserina. DNA sequence analysis of three unique "plasmids". J Mol Biol. 1985 Oct 20;185(4):659–680. doi: 10.1016/0022-2836(85)90052-x. [DOI] [PubMed] [Google Scholar]
- Jin T., Sonnenberg A. S., Van Griensven L. J., Horgen P. A. Investigation of Mitochondrial Transmission in Selected Matings between Homokaryons from Commercial and Wild-Collected Isolates of Agaricus bisporus (= Agaricus brunnescens). Appl Environ Microbiol. 1992 Nov;58(11):3553–3560. doi: 10.1128/aem.58.11.3553-3560.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moulinier T., Barroso G., Labarère J. The mitochondrial genome of the basidiomycete Agrocybe aegerita: molecular cloning, physical mapping and gene location. Curr Genet. 1992 May;21(6):499–505. doi: 10.1007/BF00351660. [DOI] [PubMed] [Google Scholar]
- Salvado J. C., Labarère J. Expression of Proteins and Glycoproteins Encoded by the Haploid Nuclei in the Dikaryotic State in the Basidiomycete Agrocybe aegerita. Appl Environ Microbiol. 1991 Sep;57(9):2714–2723. doi: 10.1128/aem.57.9.2714-2723.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Specht C. A., Novotny C. P., Ullrich R. C. Mitochondrial DNA of Schizophyllum commune: restriction map, genetic map, and mode of inheritance. Curr Genet. 1992 Aug;22(2):129–134. doi: 10.1007/BF00351472. [DOI] [PubMed] [Google Scholar]
- Weber C. A., Hudspeth M. E., Moore G. P., Grossman L. I. Analysis of the mitochondrial and nuclear genomes of two basidiomycetes, Coprinus cinereus and Coprinus stercorarius. Curr Genet. 1986;10(7):515–525. doi: 10.1007/BF00447385. [DOI] [PubMed] [Google Scholar]