Abstract
Eight strains of the entomopathogen Bacillus popilliae were analyzed by pulsed-field gel electrophoresis, and genomic size estimates of ^sim2,600 to 3,500 kb were obtained. The type strain, ATCC 14706, had a genomic size of 3,395 kb. For the six New Zealand isolates, the degree of similarity in the pulsed-field gel electrophoresis fingerprints may correlate with the geographical closeness of the sites of isolation. The plasmid profiles of the New Zealand isolates were also compared; four of the six strains carry plasmids in the 3.6- to 9.7-kb size range.
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- Amjad M., Castro J. M., Sandoval H., Wu J. J., Yang M., Henner D. J., Piggot P. J. An SfiI restriction map of the Bacillus subtilis 168 genome. Gene. 1991 May 15;101(1):15–21. doi: 10.1016/0378-1119(91)90219-2. [DOI] [PubMed] [Google Scholar]
- Bakhiet N., Stahly D. P. Studies on transfection and transformation of protoplasts of Bacillus larvae, Bacillus subtilis, and Bacillus popilliae. Appl Environ Microbiol. 1985 Mar;49(3):577–581. doi: 10.1128/aem.49.3.577-581.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Canard B., Cole S. T. Genome organization of the anaerobic pathogen Clostridium perfringens. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6676–6680. doi: 10.1073/pnas.86.17.6676. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carlson C. R., Grønstad A., Kolstø A. B. Physical maps of the genomes of three Bacillus cereus strains. J Bacteriol. 1992 Jun;174(11):3750–3756. doi: 10.1128/jb.174.11.3750-3756.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carlson C. R., Kolstø A. B. A small (2.4 Mb) Bacillus cereus chromosome corresponds to a conserved region of a larger (5.3 Mb) Bacillus cereus chromosome. Mol Microbiol. 1994 Jul;13(1):161–169. doi: 10.1111/j.1365-2958.1994.tb00411.x. [DOI] [PubMed] [Google Scholar]
- Chakrabarti S., Brechling K., Moss B. Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques. Mol Cell Biol. 1985 Dec;5(12):3403–3409. doi: 10.1128/mcb.5.12.3403. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dingman D. W. Presence of N6-methyladenine in GATC sequences of Bacillus popilliae and Bacillus lentimorbus KLN2. J Bacteriol. 1990 Oct;172(10):6156–6159. doi: 10.1128/jb.172.10.6156-6159.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
- McClelland M., Jones R., Patel Y., Nelson M. Restriction endonucleases for pulsed field mapping of bacterial genomes. Nucleic Acids Res. 1987 Aug 11;15(15):5985–6005. doi: 10.1093/nar/15.15.5985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rhodes R. A., Roth M. S., Hrubant G. R. Sporulation of bacillus popilliae on solid media. Can J Microbiol. 1965 Oct;11(5):779–783. doi: 10.1139/m65-105. [DOI] [PubMed] [Google Scholar]
- Sharpe E. S., St Julian G., Crowell C. Characteristics of a new strain of Bacillus popilliae sporogenic in vitro. Appl Microbiol. 1970 Apr;19(4):681–688. doi: 10.1128/am.19.4.681-688.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stahly D. P., Takefman D. M., Livasy C. A., Dingman D. W. Selective Medium for Quantitation of Bacillus popilliae in Soil and in Commercial Spore Powders. Appl Environ Microbiol. 1992 Feb;58(2):740–743. doi: 10.1128/aem.58.2.740-743.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Valyasevi R., Kyle M. M., Christie P. J., Steinkraus K. H. Plasmids in Bacillus popilliae. J Invertebr Pathol. 1990 Sep;56(2):286–288. doi: 10.1016/0022-2011(90)90113-k. [DOI] [PubMed] [Google Scholar]
- Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
