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. 1995 Dec;61(12):4343–4347. doi: 10.1128/aem.61.12.4343-4347.1995

Isolation of Multiple Subspecies of Bacillus thuringiensis from a Population of the European Sunflower Moth, Homoeosoma nebulella

C Itoua-Apoyolo, L Drif, J M Vassal, H DeBarjac, J P Bossy, F Leclant, R Frutos
PMCID: PMC1388652  PMID: 16535187

Abstract

Five subspecies of Bacillus thuringiensis were isolated from dead and diseased larvae obtained from a laboratory colony of the European sunflower moth, Homoeosoma nebulella. The subspecies isolated were B. thuringiensis subspp. thuringiensis (H 1a), kurstaki (H 3a3b3c), aizawai (H 7), morrisoni (H 8a8b), and thompsoni (H 12). Most isolates produced typical bipyramidal crystals, but the B. thuringiensis subsp. thuringiensis isolate produced spherical crystals and the B. thuringiensis subsp. thompsoni isolate produced a pyramidal crystal. Analysis of the parasporal crystals by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the crystals from the B. thuringiensis subsp. kurstaki and aizawai isolates contained a protein of 138 kDa whereas those from B. thuringiensis subsp. morrisoni contained a protein of 145 kDa. The crystals from B. thuringiensis subsp. thuringiensis contained proteins of 125, 128, and 138 kDa, whereas those from B. thuringiensis subsp. thompsoni were the most unusual, containing proteins of 37 and 42 kDa. Bioassays of purified crystals conducted against second-instar larvae of H. nebulella showed that the isolates of B. thuringiensis subspp. aizawai, kurstaki, and thuringiensis were the most toxic, with 50% lethal concentrations (LC(inf50)s) of 0.15, 0.17, and 0.26 (mu)g/ml, respectively. The isolates of B. thuringiensis subspp. morrisoni and thompsoni had LC(inf50)s of 2.62 and 37.5 (mu)g/ml, respectively. These results show that a single insect species can simultaneously host and be affected by a variety of subspecies of B. thuringiensis producing different insecticidal proteins.

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Selected References

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  1. Aronson A. I., Beckman W., Dunn P. Bacillus thuringiensis and related insect pathogens. Microbiol Rev. 1986 Mar;50(1):1–24. doi: 10.1128/mr.50.1.1-24.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aronson A. I. The two faces of Bacillus thuringiensis: insecticidal proteins and post-exponential survival. Mol Microbiol. 1993 Feb;7(4):489–496. doi: 10.1111/j.1365-2958.1993.tb01139.x. [DOI] [PubMed] [Google Scholar]
  3. Brown K. L., Whiteley H. R. Molecular characterization of two novel crystal protein genes from Bacillus thuringiensis subsp. thompsoni. J Bacteriol. 1992 Jan;174(2):549–557. doi: 10.1128/jb.174.2.549-557.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burges H. D. Enzootic diseases of insects. Ann N Y Acad Sci. 1973 Jun 22;217:31–49. doi: 10.1111/j.1749-6632.1973.tb32746.x. [DOI] [PubMed] [Google Scholar]
  5. DeLucca A. J., 2nd, Simonson J. G., Larson A. D. Bacillus thuringiensis distribution in soils of the United States. Can J Microbiol. 1981 Sep;27(9):865–870. doi: 10.1139/m81-137. [DOI] [PubMed] [Google Scholar]
  6. Höfte H., Whiteley H. R. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol Rev. 1989 Jun;53(2):242–255. doi: 10.1128/mr.53.2.242-255.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kaelin P., Morel P., Gadani F. Isolation of Bacillus thuringiensis from Stored Tobacco and Lasioderma serricorne (F.). Appl Environ Microbiol. 1994 Jan;60(1):19–25. doi: 10.1128/aem.60.1.19-25.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Martin P. A., Travers R. S. Worldwide Abundance and Distribution of Bacillus thuringiensis Isolates. Appl Environ Microbiol. 1989 Oct;55(10):2437–2442. doi: 10.1128/aem.55.10.2437-2442.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Padua L. E., Federici B. A. Development of mutants of the mosquitocidal bacterium Bacillus thuringiensis subspecies morrisoni (PG-14) toxic to lepidopterous or dipterous insects. FEMS Microbiol Lett. 1990 Jan 1;54(1-3):257–262. doi: 10.1016/0378-1097(90)90293-y. [DOI] [PubMed] [Google Scholar]
  11. Smith R. A., Couche G. A. The Phylloplane as a Source of Bacillus thuringiensis Variants. Appl Environ Microbiol. 1991 Jan;57(1):311–315. doi: 10.1128/aem.57.1.311-315.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tailor R., Tippett J., Gibb G., Pells S., Pike D., Jordan L., Ely S. Identification and characterization of a novel Bacillus thuringiensis delta-endotoxin entomocidal to coleopteran and lepidopteran larvae. Mol Microbiol. 1992 May;6(9):1211–1217. doi: 10.1111/j.1365-2958.1992.tb01560.x. [DOI] [PubMed] [Google Scholar]

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